Adilah Ayoib
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Preferred name
Adilah Ayoib
Official Name
Ayoib, Adilah
Alternative Name
Ayoib, Adilah
Ayoib, A.
Main Affiliation
Scopus Author ID
57186613000
Researcher ID
GWV-4156-2022

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  • Publication
    Automated, high-throughput DNA extraction protocol for disposable label free, microfluidics integrating DNA biosensor for oil palm pathogen, Ganoderma boninense
    ( 2020-05-01)
    Adilah Ayoib
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    Basal Stem Rot and Upper Stem Rot diseases caused by pathogenic fungus Ganoderma boninense continue to be a major plight in the palm oil industry. Despite continuous research in combating the problem, resolution remains stagnant. Here, developed an automated, high-throughput DNA extraction protocol on microfluidics device for a quick, disposable, label-free detection, within 2 h of assessment. Microfluidics was designed using AutoCAD software, fabricated on microscopic glass substrate using negative photoresist (SU-8 2015) and molded with a biopolymer silicone, Polydimethylsiloxane. G. boninense and unknown pathogenic fungus isolated from rotten mushroom were grown and fractions of extracted DNA were pooled and analyzed for comparison along with synthetic ssDNA of G. boninense. Results from LPM and HPM show successful fabrication with ≤0.1 mm variance between the dimensions in the design before and after lithography process. The PDMS microfluidics show no leakage when run with DNA samples. Analyses from I-V measurement, UV–vis, FTIR, and PCR show comparable results between extracted and synthetic ssDNA of G. boninense and a contrast with the unknown pathogenic fungus, indicating a successful DNA extraction protocol via microfluidics for label-free identification of G. boninense. Optimization of DNA extraction can be further devised for applicability on lab-on-a-chip devices.
  • Publication
    Distinct Detection of Ganoderma Boninense On Metal Oxides-Gold Nanoparticle Composite Deposited Interdigitated Electrode DNA sensor
    ( 2021-12-14)
    Thivina V.
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Adilah Ayoib
    ;
    Nordin N.K.S.
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    Oil palms suffer severe losses due to Ganoderma boninense infection that causes Basal Stem Rot (BSR). The available detection measuring the severity of BSR disease have not proved satisfactory output. Due to the influence of oil palm industry in country's economy, effective and efficient means of diagnostic measure is mandatory. Among the available diagnostic tools, biosensors were redeemed to yield the most rapid and selective results. To overcome the current issues, herein Interdigitated Electrode (IDE) electrochemical DNA biosensor to detect Ganoderma boninense was successfully designed and fabricated by thermal deposition. Lift-off photolithography fabrication process was applied followed by the surface chemical functionalization via seed deposition. Zinc Oxide (ZnO) and Titanium Dioxide (TiO2) were overlaid and the functionalized metal oxides IDE surfaces were used to detect DNA sequence complementation from Ganoderma boninense. Furthermore, gold nanoparticles were doped to increase the surface to volume ratio and enhance biocompatibility. Characterizations were made by validating the sensor's topology characteristics and electrical characteristics. From the results recorded, it has been justified that IDE with ZnO doped with gold nanoparticles surface serves as an excellent DNA sensor for the detection of Ganoderma boninense with a remarkable current of 290 nA and 176 nA for immobilization and hybridization respectively.
  • Publication
    Cost-Effective Fabrication of Polydimethylsiloxane (PDMS) Microfluidics for Point-of-Care Application
    ( 2024-06-01)
    Adilah Ayoib
    ;
    Karim N.A.A.A.
    ;
    Uda Hashim
    ;
    Shamsuddin S.A.
    ;
    Abd Rahman S.F.
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Nor Azizah Parmin
    Microfluidics fabrication pertains to the construction of small-scale devices and systems that manipulate and control small volumes of fluids. This process involves precise engineering and manufacturing procedures aimed at designing and producing these devices, which find applications in healthcare, environmental monitoring, and chemical analysis. The present study showcases an inexpensive approach to fabricate microfluidics channels using PDMS biopolymer and soft lithography technique to achieve laminar fluid flow. Initially, a robust and adhesive mold was created by fabricating a master template using several layers of SU-8 5 and SU-8 2015 negative photoresists. Subsequently, PDMS microfluidics channels were replicated and sealed onto a glass substrate through plasma bonding treatment. High-power microscopy images and profilometer analyses demonstrated successful fabrication with minimal deviation from the initial designs and the fabricated devices (less than 0.07 mm, less than 0.6°). Both the SU-8 master template and PDMS replicate displayed average microchannel height values and surface roughness of 100 μm and 0.26 µm or lower, respectively. Additionally, the fluid test confirmed laminar flow without any leakage post plasma oxidation, indicating the completion of an efficient and cost-effective fabrication process.
  • Publication
    Cost-effective fabrication of polydimethylsiloxane (PDMS) microfluidics for point-of-care application
    ( 2024-06)
    Adilah Ayoib
    ;
    Noor Amalina Aini Abdul Karim
    ;
    Uda Hashim
    ;
    Shahidah Arina Shamsuddin
    ;
    Siti Fatimah Abd Rahman
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Nor Azizah Parmin
    Microfluidics fabrication pertains to the construction of small-scale devices and systems that manipulate and control small volumes of fluids. This process involves precise engineering and manufacturing procedures aimed at designing and producing these devices, which find applications in healthcare, environmental monitoring, and chemical analysis. The present study showcases an inexpensive approach to fabricate microfluidics channels using PDMS biopolymer and soft lithography technique to achieve laminar fluid flow. Initially, a robust and adhesive mold was created by fabricating a master template using several layers of SU-8 5 and SU-8 2015 negative photoresists. Subsequently, PDMS microfluidics channels were replicated and sealed onto a glass substrate through plasma bonding treatment. High-power microscopy images and profilometer analyses demonstrated successful fabrication with minimal deviation from the initial designs and the fabricated devices (less than 0.07 mm, less than 0.6°). Both the SU-8 master template and PDMS replicate displayed average microchannel height values and surface roughness of 100 μm and 0.26 μm or lower, respectively. Additionally, the fluid test confirmed laminar flow without any leakage post plasma oxidation, indicating the completion of an efficient and cost-effective fabrication process.
  • Publication
    High efficiency carry save adder using modified–gate diffusion input technique
    ( 2024-06)
    Teoh Yong Keong
    ;
    Siti Fatimah Abd Rahman
    ;
    Mohamed Fauzi Packeer Mohamed
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Adilah Ayoib
    ;
    Thikra S. Dhahi
    Addition is a fundamental function in the design of a digital system, necessary for applications such as signal processing, arithmetic operations, multiplexers, and control systems. Hence, the digital system’s performance is considerably reliant on the efficiency of the adders. Therefore, designing a 4-bit carry save adder (CSA) that consumes less power, occupies a smaller area, and operates at a higher speed is proposed using the modified–gate diffusion input (MOD–GDI) technique. The primary focus is to reduce the area occupied by decreasing the transistor count as compared with other logic styles (i.e., conventional, and Boolean simplification) for CSA through Cadence Virtuoso simulation based on SilTerra 180 nm technology. Notably, the number of transistors is reduced from 42 in the conventional full adder to 11 in the proposed MOD–GDI design. As a result, the proposed 4-bit CSA with MOD–GDI technique is efficient in improving the speed of addition by reducing the area and power consumption.
  • Publication
    Automated, high-throughput DNA extraction protocol for disposable label free, microfluidics integrating DNA biosensor for oil palm pathogen, Ganoderma boninense
    ( 2020-05-01)
    Adilah Ayoib
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    Basal Stem Rot and Upper Stem Rot diseases caused by pathogenic fungus Ganoderma boninense continue to be a major plight in the palm oil industry. Despite continuous research in combating the problem, resolution remains stagnant. Here, developed an automated, high-throughput DNA extraction protocol on microfluidics device for a quick, disposable, label-free detection, within 2 h of assessment. Microfluidics was designed using AutoCAD software, fabricated on microscopic glass substrate using negative photoresist (SU-8 2015) and molded with a biopolymer silicone, Polydimethylsiloxane. G. boninense and unknown pathogenic fungus isolated from rotten mushroom were grown and fractions of extracted DNA were pooled and analyzed for comparison along with synthetic ssDNA of G. boninense. Results from LPM and HPM show successful fabrication with ≤0.1 mm variance between the dimensions in the design before and after lithography process. The PDMS microfluidics show no leakage when run with DNA samples. Analyses from I-V measurement, UV–vis, FTIR, and PCR show comparable results between extracted and synthetic ssDNA of G. boninense and a contrast with the unknown pathogenic fungus, indicating a successful DNA extraction protocol via microfluidics for label-free identification of G. boninense. Optimization of DNA extraction can be further devised for applicability on lab-on-a-chip devices.
  • Publication
    Impact of Nanogap Thickness on Dielectric-Modulated Field-effect Transistor Biosensor Performance for Uncharged Biomolecules Detection
    ( 2023-01-01)
    Jasmi M.S.
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Mohd Khairuddin Md Arshad
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Halim N.H.A.
    ;
    Rahman S.F.A.
    ;
    Adilah Ayoib
    ;
    Shaifullah. A. S M.
    ;
    Ibrahim M.M.
    Uncharged biomolecules sensing performance of dielectric-modulated field-effect transistor (DMFET) biosensor at various nanogap thickness via semiconductor device simulation tool was assessed in this work. The device structures with 10 nm-, 15 nm-, and 20 nm-thick nanogap were constructed for this investigation. Each device structure was applied with dielectric constant ranging from 2 to 7 at the nanogap representing the presence of various biomolecules. These device structures were electrically simulated by supplying gate voltage from 0 V to 2 V and biased with drain voltage of 0.05 V for linear region of operation. Based on the extracted drain current, the reduction of nanogap thickness increase capacitance at the nanogap region. In additional, increase in nanogap's dielectric constant causing an increase of its capacitance, and translated into higher output drain current. Sensitivity calculation and analysis shows DMFET biosensor with 10 nm-thick nanogap demonstrated the highest sensitivity with 6.896 μA/dec, which possibly permit enhanced sensing of uncharged biomolecule.