Nur Hamidah Abdul Halim
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Preferred name
Nur Hamidah Abdul Halim
Official Name
Nur Hamidah, Abdul Halim
Alternative Name
Halim, Nur Hamidah Abdul
Halim, N. H.A.
Hamidah A Halim, N.
Halim, N. H.
Halim, N. Hamidah A.
Abdul Halim, Nur Hamidah
Main Affiliation
Scopus Author ID
55493444600

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  • Publication
    Immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor for stable cortisol quantification in serum
    ( 2020-12-01)
    Nur Nasyifa M.M.
    ;
    Ruslinda A. Rahim
    ;
    Nur Hamidah Abdul Halim
    ;
    Zainol Abidin A.S.
    ;
    Mohd Faudzi F.N.
    ;
    Ahmad N.A.
    ;
    Lockman Z.
    ;
    Rezek B.
    ;
    Kromka A.
    ;
    Subash Chandra Bose Gopinath
    Physiological and emotional stress affects the regulation of cortisol secretion, a routine process in circadian rhythm. Regular monitoring of cortisol level as a biomarker in the blood stream becomes vital to determine cortisol-related diseases. This study reports immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor (EGFET) biosensor for cortisol determination in human serum. Solution-processed graphene nanoplatelets were evidenced on the surface by Raman spectroscopy analysis and utilized as the transducing element on the field-effect transistor. Further, confirmed the binding events of the antibody on graphene nanoplatelets using X-Ray Photoelectron Spectroscopy and characterized the electrostatic gating effect of cortisol and intermediate functionalization on graphene nanoplatelets-EGFET. The biosensor exhibited good sensitivity of 72.30 µA.(g/mL)−1 in a linear range between 1.00 pg/mL to 10.00 ng/mL, with a limit of detection (LOD) of 0.85 pg/mL. Confirmation with binding events on the biosensor was done using the relevant molecules, progesterone, cortisone, and corticosterone, and found to be selective towards cortisol. Cortisol was also successfully detected with interference by the human serum, suggesting the capability of graphene nanoplatelets-EGFET sensor for determining cortisol in a complex matrix.
      5  24
  • Publication
    Fabrication and simulation of silicon nanogaps pH sensor as preliminary study for Retinol Binding Protein 4 (RBP4) detection
    (Universiti Malaysia Perlis (UniMAP), 2025-01)
    M. I. Hashim
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Mohamad Faris Mohamad Fathil
    ;
    M. Shaifullah A.S
    ;
    C. Y. Chean
    ;
    Nur Hamidah Abdul Halim
    ;
    Zarimawaty Zailan
    ;
    Mohd Khairuddin Md Arshad
    ;
    Muzamir Isa
    ;
    Ayu Wazira Azhari
    ;
    M. Syamsul
    ;
    Rozaimah A.T.
    In this research, a silicon nanogap biosensor has the potential to play a significant role in the field of biosensors for detecting Retinol Binding Protein 4 (RBP4) molecules due to its unique nanostructure morphology, biocompatibility features, and electrical capabilities. Additionally, as preliminary research for RBP4, a silicon nanogap biosensor with unique molecular gate control for pH measurement was developed. Firstly, using conventional lithography followed by the Reactive-ion etching (RIE) technique, a nanofabrication approach was utilized to produce silicon nanogaps from silicon-on-insulator (SOI) wafers. The critical aspects contributing to the process and size reduction procedures were highlighted to achieve nanometer-scale size. The resulting silicon nanogaps, ranging from 100 nm to 200 nm, were fabricated precisely on the device. Secondly, pH level detection was performed using several types of standard aqueous pH buffer solutions (pH 6, pH 7, pH 12) to test the electrical response of the device. The sensitivity of the silicon nanogap pH sensor was 7.66 pS/pH (R² = 0.97), indicating that the device has a wide range of pH detecting capacity. This also includes the silicon nanogap biosensor validated by simulation, with the sensitivity obtained being 3.24 μA/e.cm² (R² = 0.98). The simulation of the sensitivity is based on the interface charge (Qf) that represents the concentration of RBP4. The results reveal that the silicon nanogap biosensor has excellent characteristics for detecting pH levels and RBP4 with outstanding sensitivity performance. In conclusion, this silicon nanogap biosensor can be used as a new electrical RBP4 biosensor for biomedical diagnostic applications in the future.
      3  21
  • Publication
    Silicon nanowire biosensors for diabetes mellitus monitoring
    ( 2024-10)
    M. Shaifullah A. S
    ;
    J. Jumat
    ;
    Mohammad Nuzaihan Md Nor
    ;
    J. N. Ismail
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Nur Hamidah Abdul Halim
    ;
    Zarimawaty Zailan
    ;
    Mohd Khairuddin Md Arshad
    ;
    M. Syamsul
    ;
    Rozaimah A. T
    The main goal of this research is the development of a label-free biosensor for the detection of diabetes mellitus (DM) using the target molecule retinol-binding protein 4 (RBP4). The enzyme-linked immunosorbent assay (ELISA) approach, currently used to detect DM, is time-consuming and difficult. As a result, label-free biosensors are being considered as an alternative. In this research, silicon nanowires (SiNWs) were selected as the transducer for this biosensor due to their low cost, real-time analysis capability, high sensitivity, and low detection limit. The SiNWs were created using conventional lithography, reactive ion etching (RIE), and physical vapor deposition (PVD), and then dripped with a gold nanoparticle solution to create gold-decorated SiNWs. The surface of the gold-decorated SiNWs was functionalized using 3-aminothiophenol and glutaraldehyde solutions before being immobilized with DM RBP4 antibodies and targets. The electrical characterization of the gold nanoparticle decorated SiNWs biosensor revealed good performance in DM detection. The pH tests confirmed that the SiNWs acted as a transducer, with current proportional to the DM RBP4 concentration. The estimated limit of detection (LOD) and sensitivity for detecting DM RBP4 binding were 0.076 fg/mL and 8.92 nA(g/mL)-1, respectively. This gold nanoparticle decorated SiNWs biosensor performed better than other methods and enabled efficient, accurate, and direct detection of DM. The SiNWs could be used as a distinctive electrical protein biosensor for biological diagnostic purposes. In conclusion, gold nanoparticle deposition offers effective label-free, direct, and high-accuracy DM detection, outperforming previous approaches. Thus, these SiNWs serve as novel electrical protein biosensors for future biological diagnostic applications.
      26  3
  • Publication
    Micro-interdigitated electrodes genosensor based on Au-deposited nanoparticles for early detection of cervical cancer
    (Elsevier, 2023-12-31)
    Jaapar F. Nadhirah
    ;
    Nor Azizah Parmin
    ;
    Nur Hamidah Abdul Halim
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Fatin Syakirah Halim
    ;
    Muhammad Nur Aiman Uda
    ;
    Amilia Afzan
    ;
    Noorhashimah Mohamad Nor
    ;
    Khairunisak Abdul Razak
    Genosensor-based electrodes mediated with nanoparticles (NPs) have tremendously developed in medical diagnosis. Herein, we report a facile, rapid, low cost and highly sensitive biosensing strategy for early detection of HPV 18 using gold-nanoparticles (AuNPs) deposited on micro-IDEs. This study represents surface charge transduction of micro-interdigitated electrodes (micro-IDE) alumina insulated with silica, independent and mini genosensor modified with colloidal gold NPs (AuNPs), and determination of gene hybridization for early detection of cervical cancer. The surface of AuNPs deposited micro-IDE functionalized with optimized 3-aminopropyl-triethoxysilane (APTES) followed by hybridization with deoxyribonucleic acid (DNA) virus to develop DNA genosensor. The results of ssDNA hybridization with the ssDNA target of human papillomavirus (HPV) 18 have affirmed that micro-IDE functionalized with colloidal AuNPs resulted in the lowest detection at 0.529 aM. Based on coefficient regression, micro-IDE functionalized with AuNPs produces better results in the sensitivity test (R2 = 0.99793) than unfunctionalized micro-IDE.
      7  27