Publication:
Micro-interdigitated electrodes genosensor based on Au-deposited nanoparticles for early detection of cervical cancer
Micro-interdigitated electrodes genosensor based on Au-deposited nanoparticles for early detection of cervical cancer
Date
2023
Authors
Jaapar F. Nadhirah
Nor Azizah Parmin
Nur Hamidah Abdul Halim
Uda Hashim
Subash Chandra Bose Gopinath
Fatin Syakirah Halim
Muhammad Nur Aiman Uda
Amilia Afzan
Noorhashimah Mohamad Nor
Khairunisak Abdul Razak
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Research Projects
Organizational Units
Journal Issue
Abstract
Genosensor-based electrodes mediated with nanoparticles (NPs) have tremendously developed in medical diagnosis. Herein, we report a facile, rapid, low cost and highly sensitive biosensing strategy for early detection of HPV 18 using gold-nanoparticles (AuNPs) deposited on micro-IDEs. This study represents surface charge transduction of micro-interdigitated electrodes (micro-IDE) alumina insulated with silica, independent and mini genosensor modified with colloidal gold NPs (AuNPs), and determination of gene hybridization for early detection of cervical cancer. The surface of AuNPs deposited micro-IDE functionalized with optimized 3-aminopropyl-triethoxysilane (APTES) followed by hybridization with deoxyribonucleic acid (DNA) virus to develop DNA genosensor. The results of ssDNA hybridization with the ssDNA target of human papillomavirus (HPV) 18 have affirmed that micro-IDE functionalized with colloidal AuNPs resulted in the lowest detection at 0.529 aM. Based on coefficient regression, micro-IDE functionalized with AuNPs produces better results in the sensitivity test (R2 = 0.99793) than unfunctionalized micro-IDE.
Description
Keywords
Cervical cancer,
Genosensor,
Gold nanoparticles (AuNPs),
Human papillomavirus (HPV),
Interdigitated electrodes (IDEs)