Noor Hasyierah Mohd Salleh
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Noor Hasyierah Mohd Salleh
Official Name
Noor Hasyierah, Mohd Salleh
Alternative Name
Salleh, N. H.M.
Salleh, N. H.Mohd
Mohd Salleh, N. H.
Mohd Salleh, Noor Hasyierah
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41762655600

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  • Publication
    Inhibition of Aspergillus flavus by Momordica charantia Ethanolic Leaves Extract for Mushroom Protection
    ( 2023-06-09)
    Noor Hasyierah Mohd Salleh
    ;
    Zulkarnay Zakaria
    ;
    Aziz N.H.
    ;
    Ahmad Anas Nagoor Gunny
    ;
    Alina Rahayu Mohamed
    ;
    Munusamy T.
    Pleurotus ostreatus, better known as oyster mushroom is one of the world’s widely cultivated mushroom. Infection from pathogenic fungi is one of the major reasons causing losses of mushroom during cultivation. The objective of this study is to investigate the antifungal property of Momordica charantia leaves extract against Aspergillus flavus for mushroom protection. The inhibitory effect of M. charantia leaves extract against A. flavus isolated from infected P. ostreatus mushroom bag was investigated using pour plate technique. Inhibition of A. flavus mycelial growth was the highest at extract concentration of 9.64 v/v %, temperature 26℃ and incubation time of 96 hours with inhibitory value of 70.58 ± 0.00 %, 78.4 ± 0.00 % and 63.33 ± 2.89 % respectively. Structural morphology of the mycelia grown on the treated plate also showed morphological alteration compared to the control plate. Hence, Momordica charantia leaves extract may serves as a promising fungicides alternative to chemical use for controlling invasion of A. flavus on oyster mushroom.
  • Publication
    CHARACTERISATION OF POMACEA CANALICULATA EGGS TREATED WITH PROTEASE
    ( 2024-01-01)
    Noor Hasyierah Mohd Salleh
    ;
    Nurhadijah Zainalabidin
    ;
    Siti Noor Hajjar Md Latip
    Pomacea canaliculata is a type of freshwater snail that has become a major pest in paddy fields, as it feeds on young paddy leaves and stems, thus, posing a serious threat to paddy production. It was named one of the world’s top 100 worst invasive species, with serious consequences for the environment, human health, and the social economy. Their hatchability rate is high, which explains their global distribution worldwide. Therefore, it is vital to manage their hatchability to prevent their population from expanding further by understanding the protection that permits the eggs to survive. P. canaliculata eggs are covered with a thin layer of cuticle that is rich in protein to protect the embryo during the hatching process. The biological treatment with protease enzyme successfully hydrolysed the protein cuticle layer, lowering the percentage of hatchability. Disruption of the protein cuticle may have an impact on conductivity, water loss, hatching time, protein content, and other factors. However, documentation of the protease effect on the protein cuticle is scarce. Therefore, the goal of this study is to evaluate the protease treatment on the protein cuticle of P. canaliculata eggs physically (conductivity, water loss, and morphological analysis) and chemically (cuticle protein content, protein breakdown, and amino acid profile). Physical characterisation revealed that protease-treated eggs have higher conductivity and water loss than the control egg. Images taken with a light microscope (LM) and a scanning electron microscope (SEM) revealed changes in cuticle structure, which explained the protease-induced cuticle hydrolysis. Chemical characterisation revealed a decrease in cuticle protein content, hydrolysis of protein to a small size, and changes in amino acid composition. The physical and chemical analyses strongly suggested that protease can damage the cuticle protein, thus, preventing the eggs from hatching.
  • Publication
    Thermal pyrolysis of empty fruit bunch (EFB) in a vertical fixed-bed reactor
    (IOP Publishing, 2020)
    Alina Rahayu Mohamed
    ;
    A N Awang
    ;
    Noor Hasyierah Mohd Salleh
    ;
    R Badlishah Ahmad
    In the production of crude palm oil, huge quantities of solid biomass waste is generated such as empty fruit bunch (EFB), palm fronds and palm–pressed fibre (PPF). This inevitably resulted in the environmental pollution due to its natural conversion to methane. In this study, thermal pyrolysis of EFB was conducted using a fixed bed reactor. The factors such as nitrogen gas flowrate and holding time that affect the distribution of pyrolysis product yields were being investigated by evaluating the percentage product yields. It was determined that at nitrogen gas flowrate of 300 cm3/min and 30 mins holding time, the maximum bio-oil yield of 46.20 % was attained with gas and char yield of 21.79 % and 32.01 % respectively. The existence of functional groups in bio-oil was analyzed by Fourier Transfrom Infra-Red (FTIR) spectroscopy. It was identified that the bio-oil has several functional groups such as hydroxyl (OH), ketone/aldehyde (C=O) and ester (O-C=O) groups. The dynamic viscoscity of bio-oil was analyzed by Brookfield viscometer. It showed that the viscosity of bio-oil decreased with increasing temperature from 25-50 °C.
  • Publication
    Optimization of microwave sol–gel synthesis of N-Ce-AC/TiO₂ for adsorption/photodegradation of tetracycline
    (Elsevier, 2023)
    Nur Athirah Awatif Abdul Rahman
    ;
    Azduwin Khasri
    ;
    Sabah Ansar
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Mohd Ridzuan Mohd Jamir
    ;
    Raj Boopathy
    ;
    Achmad Syafiuddin
    Nitrogen (N) and cerium (Ce) co-doped titanium dioxide (TiO₂) supported activated carbon (AC) (N-Ce-AC/TiO₂) were synthesized to remove antibiotic tetracycline from aqueous solution via adsorption and photodegradation. The sol–gel technique, aided by microwave radiation, was used to synthesize N-Ce-AC/TiO₂. Central composite design under response surface methodology was used to optimize the variables comprising urea (N source) (A: 0.02–0.20 g), cerium(III) nitrate hexahydrate (Ce source) (B: 0.02–0.20 g), activated carbon (C: 0.10–0.50 g), and microwave power (D: 600–800 W), where the degradation of tetracycline was the response. Characterization of the produced catalyst was carried out by means of X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and the Brunauer–Emmett–Teller method for determining surface-texture parameters. N-Ce-AC/TiO₂ prepared with 0.50 g activated carbon, doped with 0.02 g urea and 0.20 g cerium, and activated at microwave power 600 W for 15 min exhibited 91.08% tetracycline removal when subjected to 7 W of UV irradiation, according to the results of optimal variable preparation.
  • Publication
    Protein extraction of Momordica Charantia seed assisted by ultrasound extraction
    (Springer, 2023)
    Muhamad Ikmal Sirozi
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Mohd Amin Zainal Abidin
    ;
    Norhidayah Abd Aziz
    ;
    Siti Aminah Mohd Hassan
    ;
    Zarina Zakaria
    The Momordica charantia is a tropical and subtropical plant with high nutritional value that has been implemented in traditional folk medicine as an antifungal, antimutagenic, Anthelmintic, anticancer, antifertility, and antidiabetic agent. The seeds contain rich source of antifungal protein that can be explored to be used against various fungus disease. In this study, the antifungal protein was extracted by using ultrasound assisted extraction (UAE) via one-factor-at-a-time (OFAT) technique with following the parameter sodium chloride, NaCl concentration (0.5, 1.0, 1.5, 2.0 and 2.5 M), solute ratio (0.1, 0.2, 0.3, 0.4 and 0.5 g/mL) and extraction time (4, 6, 8, 10 and 12 min). After extraction, the extract was further centrifuged and assayed by standard Bradford method before analysing the protein content at 595 nm. The result shows the optimum conditions for protein content is 6.445 mg/mL achieved after treatment with 1.5 M NaCl, 1w;10v (g/mL) in 10 min with p-value < 0.01 from each of parameter. Results showed that Momordica charantia seed had desired protein source with optimal parameter and promising antifungal bioresources with prospective applications in food and other industries.
  • Publication
    Optimization of an ultrasound-assisted extraction method for phenolic content in Momordica Charantia seeds and its antifungal activity against Pleurotus Ostreatus Green Mold Pathogen
    (Springer, 2023-09)
    Norhidayah Abd Aziz
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Nur Umi Masjida Ahmad Fauzi
    ;
    Azlina Harun Kamaruddin
    ;
    Muhamad Ikmal Sirozi
    ;
    Zarina Zakaria
    ;
    Subash Chandra Bose Gopinath
    ;
    Amira Farzana Samat
    Phenolic compounds from fruits and plant parts showed several biological properties such as antifungal activity. This study reports the optimized conditions for total phenolic content (TPC) extraction from Momordica charantia seeds using ultrasound-assisted extraction and its antifungal activity against Pleurotus pulmonarius green mold (GM) pathogens. Using water as solvent, a response surface methodology (RSM) was employed involving three independent factors including liquid to solid ratio (LSR) (10:1–60:1), pH (3.0–9.0), and extraction time (2–8 min). ANOVA suggested only parameters of pH and LSR were significant to produce TPC yield of 166.7 mg GAE/g, at optimized conditions of pH9.0 and LSR 10:1. The antifungal activity of optimized momordica seed extract (MSE) was tested against P. pulmonarius GM pathogens; Trichoderma sp., Aspergillus flavus and Penicillium citrinum. MSE presented antifungal activities against all tested strains, in a dose and strain-dependent manner. The highest growth inhibition was achieved when using 2.5 ml of extract for both Trichoderma sp. and P. citrinum with 63.1% and 89.2% inhibition, respectively. Meanwhile, 3.5 ml of extract was needed to achieve 79% inhibition on A. flavus. Conclusively, the use of non-toxic green solvent such as water, assisted with ultrasound power, is useful to produce MSE with antifungal activities as an alternative approach to chemical fungicides for the protection of grey oyster mushroom from GM pathogen.
  • Publication
    Investigating the impact of hydrophobic deep eutectic oil-in-water nanoemulsion on cell membrane degradation and inhibition of C.gloeosporioides in postharvest technology
    (Springer, 2025)
    Ahmad Anas Nagoor Gunny
    ;
    Subash Chandra Bose Gopinath
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Sunil Salleh
    ;
    Kunasundari Pareek
    This study examines the effects of a hydrophobic deep eutectic oil-in-water nanoemulsion (HyDEN) on the cell membrane degradation and inhibition of Colletotrichum gloeosporioides. By analyzing post-treatment cell membrane morphology, we gain insights into the efficacy and mechanisms of action of antifungal agents. Significant changes such as disruption, collapse, wrinkling, and lysis were observed in the external morphology of C. gloeosporioides treated with HyDEN. When comparing control and treatment groups, HyDEN demonstrated more pronounced disruption and greater mycelial growth inhibition than Globus 5.5. HyDEN also effectively inhibited spore germination compared to Globus 5.5. Tests on intracellular ion leakage showed that HyDEN caused higher conductivity, indicating significant membrane disruption. Additionally, HyDEN led to a greater release of soluble sugars due to membrane damage compared to Globus 5.5. These findings suggest that HyDEN is a promising antifungal strategy, effectively disrupting cell wall and membrane functionality, and inhibiting fungal growth.
  • Publication
    Growth Optimisation of Bacillus subtilis in medium supplemented with prebiotic gum Arabic
    ( 2020-12-18)
    Norhidayah Abd Aziz
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Abu Hassan A.F.
    ;
    Amira Farzana Samat
    ;
    Adilah Anuar
    ;
    Siti Aminah Mohd Hassan
    ;
    Lian Ou Li
    Bacillus subtilis is a type of gastrointestinal bacteria that build up a healthy gut microbiota. The bacterial species has been well documented to involve in maintaining a healthy gut homeostasis in which disruption in it's microbial composition or "dysbiosis"has recently been linked to obesity. Probiotic in vivo restoration of dysbiosed microbiota have been proved successful in obese CD-1 mice model. However, prebiotic study using Gum Arabic (GA) assessing to optimise an in vitro lumen system specifically targeting to enhance the growth of B. subtilis are still lacking since this bacteria are depleted in obese individuals. Thus, this study aimed to establish the optimal growth conditions in simulated in vitro lumen system and to the best of our knowledge, this is the first in vitro study attempted to optimize the growth of B. subtilis in medium supplemented with prebiotic GA. Growth screening analysis suggested an optimal dosage of 1.0% and 0.5% glucose and GA, respectively. The highest growth rate was recorded at 0.7995 hours-1 with doubling time of 52.02 minutes with extended period of stationary phase. The optimal GA concentration and fermentation conditions were determined at 0.67%, pH 7.4 and temperature of 37 C. The validated suggested model indicates that the supplementation of GA into an optimal fermentation systems is promising to enhance the growth of gut microflora Bacillus subtilis, for restoration of a dysbiosed gut microbiota in vitro.
      4  57
  • Publication
    Botanical insecticide of chili and ginger extract on Nilaparvata lugens, brown planthopper
    (IOP Publishing Ltd., 2020)
    Noor Hasyierah Mohd Salleh
    ;
    Norhidayah Abd Aziz
    ;
    Alina Rahayu Mohamed
    ;
    Adilah Anuar
    ;
    I Nur Humaira
    Invasion of Nilaparvata lugens or brown planthopper (BPH) in paddy fields has led to the use of excessive chemical insecticides due to its effectiveness. However, excessive applications has contributed several negative effects on the environment, farmers as well as non-targeting organisms. As an alternative, a mixture of a botanical insecticide containing mixture of chili and ginger extracts have been studied. Three parameters were studied namely the extract concentration, time of exposure and temperature of exposure. The results showed that 90% of BPH mortality was found at 40% extract concentration with 72 hr duration time of exposure at optimal temperature 30 C. HPLC analysis proved the presence of capsaicin and gingerol at peaks of 4.502 min and 11.046 min respectively. From repellency analysis, the BPH showed selective repel action against the treated paddy. This is due to the pungent odor contributed from compound of gingerol, shogaol and capsaicin presented in the mixture. Meanwhile, contact toxicity studies have successfully give 100% of mortality of BPH. Microscopic analysis have shown BPH deformities was increased by the extract concentration.
      4  28