Noor Hasyierah Mohd Salleh
Thumbnail Image
Preferred name
Noor Hasyierah Mohd Salleh
Official Name
Noor Hasyierah, Mohd Salleh
Alternative Name
Salleh, N. H.M.
Salleh, N. H.Mohd
Mohd Salleh, N. H.
Mohd Salleh, Noor Hasyierah
Main Affiliation
Scopus Author ID
41762655600

Research Output

Filters

12
Reset filters

Settings
Now showing 1 - 10 of 12
Thumbnail Image
Publication

Inhibition of Aspergillus flavus by Momordica charantia Ethanolic Leaves Extract for Mushroom Protection

2023-06-09 , Noor Hasyierah Mohd Salleh , Zulkarnay Zakaria , Aziz N.H. , Ahmad Anas Nagoor Gunny , Alina Rahayu Mohamed , Munusamy T.

Pleurotus ostreatus, better known as oyster mushroom is one of the world’s widely cultivated mushroom. Infection from pathogenic fungi is one of the major reasons causing losses of mushroom during cultivation. The objective of this study is to investigate the antifungal property of Momordica charantia leaves extract against Aspergillus flavus for mushroom protection. The inhibitory effect of M. charantia leaves extract against A. flavus isolated from infected P. ostreatus mushroom bag was investigated using pour plate technique. Inhibition of A. flavus mycelial growth was the highest at extract concentration of 9.64 v/v %, temperature 26℃ and incubation time of 96 hours with inhibitory value of 70.58 ± 0.00 %, 78.4 ± 0.00 % and 63.33 ± 2.89 % respectively. Structural morphology of the mycelia grown on the treated plate also showed morphological alteration compared to the control plate. Hence, Momordica charantia leaves extract may serves as a promising fungicides alternative to chemical use for controlling invasion of A. flavus on oyster mushroom.

View
No Thumbnail Available
Publication

Optimization of microwave sol–gel synthesis of N-Ce-AC/TiO₂ for adsorption/photodegradation of tetracycline

2023 , Nur Athirah Awatif Abdul Rahman , Azduwin Khasri , Sabah Ansar , Noor Hasyierah Mohd Salleh , Mohd Ridzuan Mohd Jamir , Raj Boopathy , Achmad Syafiuddin

Nitrogen (N) and cerium (Ce) co-doped titanium dioxide (TiO₂) supported activated carbon (AC) (N-Ce-AC/TiO₂) were synthesized to remove antibiotic tetracycline from aqueous solution via adsorption and photodegradation. The sol–gel technique, aided by microwave radiation, was used to synthesize N-Ce-AC/TiO₂. Central composite design under response surface methodology was used to optimize the variables comprising urea (N source) (A: 0.02–0.20 g), cerium(III) nitrate hexahydrate (Ce source) (B: 0.02–0.20 g), activated carbon (C: 0.10–0.50 g), and microwave power (D: 600–800 W), where the degradation of tetracycline was the response. Characterization of the produced catalyst was carried out by means of X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and the Brunauer–Emmett–Teller method for determining surface-texture parameters. N-Ce-AC/TiO₂ prepared with 0.50 g activated carbon, doped with 0.02 g urea and 0.20 g cerium, and activated at microwave power 600 W for 15 min exhibited 91.08% tetracycline removal when subjected to 7 W of UV irradiation, according to the results of optimal variable preparation.

View
Thumbnail Image
Publication

Applicability of protease and urea as a molluscicide for Pomacea canaliculata (Siput Gondang Emas)

2015 , Noor Hasyierah Mohd Salleh

Pomacea canaliculata is regarded as one of the most destructive molluscs as it causes severe damage to paddy fields. This mollusc is commonly combated by using chemical molluscicide like metaldehyde, which is known as toxic chemicals, therefore causes negative impact to the farmers and the environment. Hence, there is a great demand for a user and environmental-friendly molluscicide to reduce P. canaliculata population. The present study investigates the applicability of protease and urea as an alternative molluscicide for combating both flesh and eggs of P. canaliculata. Studies on the effect of each agent on snail eggs cover chemical and physical analyses; namely unhatchability, cuticle protein profile, water loss, conductivity, gas exchange and morphological changes. For snail flesh, it covers biological, chemical and physical studies, namely, mortality, respiration, food intake, responsiveness, movement, protein, ammonia and peroxidase level of the flesh. The studies were extended by producing cell free extract protease (CFE protease) using locally available raw material like cassava, and subsequently applied as a biomolluscicide. Likewise, studies regarding urea were also extended by using coated urea with rice husk ash and starch before subsequently applied to the paddy demonstration plot. For the flesh of P. canaliculata studies, it was found that the mortality were 31% and 90% after exposure to 3.9U/mL CFE protease and 0.02M urea respectively for 20hrs. It was also observed that the respiration rate increased proportionally with the increase of urea concentration. Additionally, the exposure to CFE protease and urea also reduced the snail appetite, responsiveness and movement as compared to the control snail. Extension of the work using coated urea for paddy plot studies saved 93% of paddy from snail attack. As for the P. canaliculata eggs studies, 71%, 52% and 35% hatchability have been suppressed after treating to 0.75U/mL commercial protease, 0.19U/mL CFE protease and 0.02M urea respectively. Besides that, the rate of conductivity, water loss and respiration increased proportionally with the increase of either commercial protease activity or urea concentration. The findings from these studies show the applicability of protease and urea as a molluscicide for both the flesh and eggs of P. canaliculata.

View
Thumbnail Image
Publication

Growth Optimisation of Bacillus subtilis in medium supplemented with prebiotic gum Arabic

2020-12-18 , Norhidayah Abd Aziz , Noor Hasyierah Mohd Salleh , Abu Hassan A.F. , Amira Farzana Samat , Adilah Anuar , Siti Aminah Mohd Hassan , Lian Ou Li

Bacillus subtilis is a type of gastrointestinal bacteria that build up a healthy gut microbiota. The bacterial species has been well documented to involve in maintaining a healthy gut homeostasis in which disruption in it's microbial composition or "dysbiosis"has recently been linked to obesity. Probiotic in vivo restoration of dysbiosed microbiota have been proved successful in obese CD-1 mice model. However, prebiotic study using Gum Arabic (GA) assessing to optimise an in vitro lumen system specifically targeting to enhance the growth of B. subtilis are still lacking since this bacteria are depleted in obese individuals. Thus, this study aimed to establish the optimal growth conditions in simulated in vitro lumen system and to the best of our knowledge, this is the first in vitro study attempted to optimize the growth of B. subtilis in medium supplemented with prebiotic GA. Growth screening analysis suggested an optimal dosage of 1.0% and 0.5% glucose and GA, respectively. The highest growth rate was recorded at 0.7995 hours-1 with doubling time of 52.02 minutes with extended period of stationary phase. The optimal GA concentration and fermentation conditions were determined at 0.67%, pH 7.4 and temperature of 37 C. The validated suggested model indicates that the supplementation of GA into an optimal fermentation systems is promising to enhance the growth of gut microflora Bacillus subtilis, for restoration of a dysbiosed gut microbiota in vitro.

View
Thumbnail Image
Publication

CHARACTERISATION OF POMACEA CANALICULATA EGGS TREATED WITH PROTEASE

2024-01-01 , Noor Hasyierah Mohd Salleh , Nurhadijah Zainalabidin , Siti Noor Hajjar Md Latip

Pomacea canaliculata is a type of freshwater snail that has become a major pest in paddy fields, as it feeds on young paddy leaves and stems, thus, posing a serious threat to paddy production. It was named one of the world’s top 100 worst invasive species, with serious consequences for the environment, human health, and the social economy. Their hatchability rate is high, which explains their global distribution worldwide. Therefore, it is vital to manage their hatchability to prevent their population from expanding further by understanding the protection that permits the eggs to survive. P. canaliculata eggs are covered with a thin layer of cuticle that is rich in protein to protect the embryo during the hatching process. The biological treatment with protease enzyme successfully hydrolysed the protein cuticle layer, lowering the percentage of hatchability. Disruption of the protein cuticle may have an impact on conductivity, water loss, hatching time, protein content, and other factors. However, documentation of the protease effect on the protein cuticle is scarce. Therefore, the goal of this study is to evaluate the protease treatment on the protein cuticle of P. canaliculata eggs physically (conductivity, water loss, and morphological analysis) and chemically (cuticle protein content, protein breakdown, and amino acid profile). Physical characterisation revealed that protease-treated eggs have higher conductivity and water loss than the control egg. Images taken with a light microscope (LM) and a scanning electron microscope (SEM) revealed changes in cuticle structure, which explained the protease-induced cuticle hydrolysis. Chemical characterisation revealed a decrease in cuticle protein content, hydrolysis of protein to a small size, and changes in amino acid composition. The physical and chemical analyses strongly suggested that protease can damage the cuticle protein, thus, preventing the eggs from hatching.

View
Thumbnail Image
Publication

Valorization of Momordica charantia seeds into phytogenically synthesized silver nanoparticles for the protection of oyster mushrooms against Trichoderma pleuroticola

2023-12-01 , Norhidayah Abd Aziz , Noor Hasyierah Mohd Salleh , Subash Chandra Bose Gopinath , Kamaruddin A.H. , Muhammad Izzat Ridzuan , Ahmad N.A.

Green mold caused by Trichoderma pleuroticola is the most invasive disease of oyster mushrooms, causing massive losses worldwide. Herein, we valorized food waste, the Momordica seed extract (MSE), into silver nanoparticles (AgNPs-MSE). The synthesis parameters were optimized, and the biogenic AgNPs showed a remarkable antifungal activity against T. pleuroticola. Quantitative and qualitative analyses showed that the MSE was rich in bioactive compounds. The highly stable AgNPs-MSE was synthesized under the following conditions: MSE–AgNO3 ratio, 5:95; synthesis time, 9 min; temperature, 60 °C; and pH, 8.0. The UV absorption spectra showed surface plasma resonance peak at 428 nm, confirming the presence of spherical nanoparticles. FTIR spectra ascertained the involvement of phytoconstituents in the MSE during synthesis, and X-ray diffraction analysis revealed a face-centered cubic (fcc) crystalline structure with an average particle size of 11.34 nm. HR-TEM analysis corroborated the spherical shape, with a nanoparticle size of 8.0 nm. EDX analysis revealed the presence of silver, carbon, oxygen, nitrogen, sulfur, and chlorine in the AgNPs-MSE, which was further substantiated by XPS analysis. A zeta potential of − 29.7 mV and PDI of 0.144 revealed the good stability and uniformity of the synthesized AgNPs-MSE. The optimized AgNPs-MSE exhibited a potent antifungal activity against T. pleuroticola at 300 ppm in vitro. SEM and TEM analyses of AgNPs-MSE-treated T. pleuroticola showed hyphal system breakage and leakage of cytoplasmic contents that leads to cell death. Therefore, the use of the AgNPs-MSE as a nanoparticle-based fungicide has the potential to effectively control T. pleuroticola for oyster mushroom protection.

View
Thumbnail Image
Publication

Durability Studies of Solid Pellets from Torrefied Leucaena Leucocephala

2020-12-18 , Kee Wei Chin , Alina Rahayu Mohamed , Zin A.M. , Noor Hasyierah Mohd Salleh , Aziz A.H.A. , Mohd Qalani Che Kasim

Torrefaction of Leucaena Leucocephala was performed in a fixed bed reactor at temperature of 300 C and 20 minutes holding time under inert environment. The torrefied Leucaena Leucocephala was pelletized with starch as the binder. Two parameters were investigated such as different ratio of starch addition to torrefied Leucaena Leucocephala pellets and the thickness of torrefied Leucaena Leucocephala pellets towards its durability. The ratios of starch addition to torrefied Leucaena Leucocephala pellets studied were 0, 5,10,15,20 and 25 wt%. Meanwhile, the studied thickness of torrefied Leucaena Leucocephala pellets were 0.3, 0.4, 0.5, 0.6 and 0.7 cm. It was observed that when the ratio of starch addition to torrefied Leucaena Leucocephala pellets was varied from 0-25 wt%, the durability increased steadily and the highest durability of 56.06% was achieved at 20 wt%. When the thickness of torrefied Leucaena Leucocephala pellets was increased from 0.3-0.7 cm, the highest durability of 93.18 % was achieved at 0.5 cm thickness. Scanning Electron Microscope (SEM) analysis was performed on the torrefied Leucaena Leucocephala pellets of 0 and 20 wt% ratio of starch addition. SEM images show the existence of some minor pores on the surface of torrefied Leucaena Leucocephala pellet at 250X and 1000X magnifications.

View
Thumbnail Image
Publication

Thermal pyrolysis of empty fruit bunch (EFB) in a vertical fixed-bed reactor

2020 , Alina Rahayu Mohamed , A N Awang , Noor Hasyierah Mohd Salleh , R Badlishah Ahmad

In the production of crude palm oil, huge quantities of solid biomass waste is generated such as empty fruit bunch (EFB), palm fronds and palm–pressed fibre (PPF). This inevitably resulted in the environmental pollution due to its natural conversion to methane. In this study, thermal pyrolysis of EFB was conducted using a fixed bed reactor. The factors such as nitrogen gas flowrate and holding time that affect the distribution of pyrolysis product yields were being investigated by evaluating the percentage product yields. It was determined that at nitrogen gas flowrate of 300 cm3/min and 30 mins holding time, the maximum bio-oil yield of 46.20 % was attained with gas and char yield of 21.79 % and 32.01 % respectively. The existence of functional groups in bio-oil was analyzed by Fourier Transfrom Infra-Red (FTIR) spectroscopy. It was identified that the bio-oil has several functional groups such as hydroxyl (OH), ketone/aldehyde (C=O) and ester (O-C=O) groups. The dynamic viscoscity of bio-oil was analyzed by Brookfield viscometer. It showed that the viscosity of bio-oil decreased with increasing temperature from 25-50 °C.

View
Thumbnail Image
Publication

Soil Macrofauna abundance in the intercropping of Mangifera Indica with aromatic plants

2023-12 , Iqbal Usamah , Lee Yit Leng , Noor Hasyierah Mohd Salleh , Norawanis Abdul Razak , Zarina Zakaria , Ras Izzati Ismail , Nur Lailina Makhtar

Soil macrofauna such as earthworms, beetles, ants, and centipedes are an important aspect of soil health as they help in the breakdown of plant residue to provide natural resources such as carbon, nitrogen, and phosphorous into the soil. Monocropping practice uses heavy machinery, pesticides, and herbicides to maintain farm productivity, which negatively impacts soil macrofauna abundance. Thus, intercropping is a sustainable practice for farmers to maintain soil health with the minimal use of external input, which can do more harm to the soil ecosystem over a long period of time. The objective of the study is to evaluate the effect of intercropping mango with pandan and lemongrass on soil macrofauna density compared to mango monocrop. The field experiment consisted of mango, mango-pandan intercrops, and mango-lemongrass intercrops. Soil macrofauna was assessed using the tropical soil biology and fertility (TSBF) monolith method. The result showed that intercropping of mango-lemongrass and mango-pandan had higher soil macrofauna density compared with mango monocrop due to the presence of intercrops providing more natural resources and a better soil environment for soil macrofauna to grow and reproduce. However, further long-term research is needed to reinforce these findings.

View
Thumbnail Image
Publication

Botanical insecticide of chili and ginger extract on Nilaparvata lugens, brown planthopper

2020 , Noor Hasyierah Mohd Salleh , Norhidayah Abd Aziz , Alina Rahayu Mohamed , Adilah Anuar , I Nur Humaira

Invasion of Nilaparvata lugens or brown planthopper (BPH) in paddy fields has led to the use of excessive chemical insecticides due to its effectiveness. However, excessive applications has contributed several negative effects on the environment, farmers as well as non-targeting organisms. As an alternative, a mixture of a botanical insecticide containing mixture of chili and ginger extracts have been studied. Three parameters were studied namely the extract concentration, time of exposure and temperature of exposure. The results showed that 90% of BPH mortality was found at 40% extract concentration with 72 hr duration time of exposure at optimal temperature 30 C. HPLC analysis proved the presence of capsaicin and gingerol at peaks of 4.502 min and 11.046 min respectively. From repellency analysis, the BPH showed selective repel action against the treated paddy. This is due to the pungent odor contributed from compound of gingerol, shogaol and capsaicin presented in the mixture. Meanwhile, contact toxicity studies have successfully give 100% of mortality of BPH. Microscopic analysis have shown BPH deformities was increased by the extract concentration.

View