Uda Hashim
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Preferred name
Uda Hashim
Official Name
Uda, Hashim
Alternative Name
Hashimb, U.
Hashim, Uda
Hashim, U.
Uda, Hashim
Main Affiliation
Scopus Author ID
22633937800
Researcher ID
CVC-6955-2022

Research Output

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Now showing 1 - 10 of 43
  • Publication
    Conductometric immunosensor for specific Escherichia coli O157:H7 detection on chemically funcationalizaed interdigitated aptasensor
    ( 2024)
    Muhammad Nur Afnan Uda
    ;
    Alaa Kamal Yousif Dafhalla
    ;
    Thikra S. Dhahi
    ;
    Tijjani Adam
    ;
    Subash Chandra Bose Gopinath
    ;
    Asral Bahari ambek
    ;
    Muhammad Nur Aiman Uda
    ;
    Nor Azizah Parmin
    ;
    Nur Hulwani Ibrahim
    ;
    Uda Hashim
    Escherichia coli O157:H7 is a strain of Escherichia coli known for causing foodborne illness through the consumption of contaminated or raw food. To detect this pathogen, a conductometric immunosensor was developed using a conductometric sensing approach. The sensor was con-structed on an interdigitated electrode and modified with a monoclonal anti-Escherichia coli O157: H7 aptamer. A total of 200 electrode pairs were fabricated and modified to bind to the target molecule replica. The binding replica, acting as the bio-recognizer, was linked to the electrode surface using 3-Aminopropyl triethoxysilane. The sensor exhibited excellent performance, detecting Escherichia coli O157:H7 in a short time frame and demonstrating a wide detection range of 1 fM to 1 nM. Concentrations of Escherichia coli O157:H7 were detected within this range, with a minimum detection limit of 1 fM. This innovative sensor offers simplicity, speed, high sensitivity, selectivity, and the potential for rapid sample processing. The potential of this pro-posed biosensor is particularly beneficial in applications such as drug screening, environmental monitoring, and disease diagnosis, where real-time information on biomolecular interactions is crucial for timely decision-making and where cross-reactivity or interference may compromise the accuracy of the analysis.
  • Publication
    Electrical DNA Biosensor Using Aluminium Interdigitated Electrode for Salmonella Detection
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    Nowadays there are many alternative methods that have been discovered and developed for the rapid detection of foodborne pathogens that can cause food poisoning. Unfortunately, majority of them still requires improvement in sensitivity and selectivity issues to be of any practical use daily. In this research, biosensors was prepared from 5 m gap Aluminium interdigitated electrode (Al IDE) to detect Salmonella enterica typhi (S. typhi). The IDE sensors in the biosensor field is extremely interest in these days due to the high number of finger electrodes as comb structure which can gain high sensitivity through electrical measurements. S. typhi is a serious food borne pathogen, makes typhoid disease which causes many deaths annually in worldwide. Functionalization steps of the Al IDE to create biosensor was based on silanization by APTES, immobilization with carboxylic functionalized S. typhi ssDNA probes and blocking agent with tween-20 were the major functionalization steps. The functionalized steps were electrically characterized using current voltage measurements. The selectivity measurement was performed with specific target was identified electrically using complementary, non-complementary and single base mismatch ssDNA target.
  • Publication
    Design Low Noise Voltage Amplifier for Hand-Held Electronic Reader
    ( 2020-07-09)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    There are limited number of electrical based two type electrode electronic readers for biosensors are commercial available because of the noise issues and amplification at nano to pico ampere current range. This research is mainly focused on designing an active low pass filter circuit of electronic reader for biosensors. The entire circuits are comprised of a voltage converter circuit, active low pass filter circuit, voltage amplifier, microcontroller and display unit. The circuit capture, filter and amplify nano and pico ampere current convert it to detectable voltage range as an output signal to the processing circuit. NodeMCU was act as the process and control circuit to read the output voltage from the amplifier circuit. The signal generator will act as a replacement for the biosensor input current and oscilloscope will display the input and output signal. The Design Spark PCB software was used to design the voltage amplifier circuit. Arduino software was used to create a programming code to upload in NodeMCU microcontroller.
  • Publication
    Design 5.0 µm Gap Aluminium Interdigitated Electrode for Sensitive pH Detection
    ( 2020-07-09)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Nor Azizah Parmin
    ;
    Bakar A.H.A.
    ;
    Anuar A.
    ;
    Bakar M.A.A.
    ;
    Sulaiman M.K.
    The aim of the research study to design high sensitive biosensor for medical applications. IDE pattern was designed using AutoCAD software with 5 µm ginger gap. The fabrication process was done using a conventional photolithography process and standard CMOS process. The fabricated electrode was physically characterized using a low power microscope (LPM) and a high power microscope (HPM). The electrically validated through I-V measurements and chemically tested with different pH buffer solutions. Al IDE was well fabricated with 0.1 µm tolerance between the design mask and fabricated IDEs. Electrical measurements confirmed that IDE was well fabricated without any shortage and results of similar IDE samples were confirmed that the repeatability of the device. The extremely small current variations in nano ampere range were quantitatively detected using an extra small volume of 2 µl for different pH buffer solutions. It is confirmed that IDEs are sensitive in both alkali and hydroxyl ions medium.
  • Publication
    Aluminium Interdigitated Electrode Based Biosensor for Specific ssDNA Target Listeria Detection
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Bahrin M.A.F.
    Nowadays interdigitated electrode (IDE) based sensor have stimulated increasing interest in the application of biosensor filed. A large number of finger electrodes as comb structure gain high sensitivity through electrical measurements. In this paper, we have investigated Listeria bacteria detection through the electrical based IDE. Listeria monocytogenes is a food borne pathogen-based bacterium that can cause dangerous disease to human, some infection may result in death. The AutoCAD software was used to design the chrome mask of IDE sensor and the fabrication process was done using conventional photolithography method. The fabricated Al IDE morphologically analyzed using a low power microscope (LPM), a high-power microscope (HPM) and 3D profiler. Functionalization step of the Al IDE, silanization process was done using (3-Aminopropyl) triethoxysilane (APTES), immobilization process was done using carboxylic probe Listeria and Tween-20 as a blocking agent for nonspecific binding on the non-immobilized area of the biosensor surface. The biosensor was validated with complementary, non-complementary and single base mismatch ssDNA targets. Different concentration of complementary ssDNA target from 1 fM to 1 M was done for the sensitivity detection.
  • Publication
    Determination of Silicon Electrical Properties Using First Principles Approach
    ( 2021-12-14)
    Uda Hashim
    ;
    Tijjani Adam
    ;
    Muhammad Nur Afnan Uda
    ;
    Muhammad Nur Aiman Uda
    Silicon nanowires have attracted attention as basis for reconfigurable electronics. However, as the size decreases, the electronic properties of the nanowires vary as a result of confinement, strain and crystal topology effects. Thus, at the thin diameter regime the band gap of Silicon nanowires can no longer be derived from a simple extrapolation of the isotropic bulk behaviour. This study compares band gap parameters in sub 10nm nanowires obtained from first-principles density-functional band structure calculations with extrapolations using continuum theory in order to rationalize the changes of the overall conductance, resistance and band gap. The device consists of silicon nanowire of size between 1 nm to 6nm. The results indicate an increase of, both the energy gap and the resistance along with reduced conductivity for the thinnest wires and a dependence on the crystal orientation with gaps reaching up to 4.3 eV along <111>, 4.0 eV along <110>, and 3.7 along <100>.
  • Publication
    Production and characterization of silica nanoparticles from fly ash: conversion of agro-waste into resource
    ( 2021-01-01)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Uda Hashim
    ;
    Halim N.H.
    ;
    Parmin N.A.
    ;
    Muhammad Nur Afnan Uda
    ;
    Anbu P.
    A chemical method to synthesize amorphous silica nanoparticles from the incinerated paddy straw has been introduced. The synthesis was conducted through the hydrolysis by alkaline-acidic treatments. As a result, silica particles produced with the sizes were ranging at 60–90 nm, determined by high-resolution microscopy. The crystallinity was confirmed by surface area electron diffraction. Apart from that, chemical and diffraction analyses for both rice straw ash and synthesized silica nanoparticles were conducted by X-ray diffraction and Fourier-transform infrared spectroscopy. The percentage of silica from the incinerated straw was calculated to be 28.3. The prominent surface chemical bonding on the generated silica nanoparticles was with Si–O–Si, stretch of Si–O and symmetric Si–O bonds at peaks of 1090, 471, and 780 cm−1, respectively. To confirm the impurities of the elements in the produced silica, were analyzed using X-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. The stability of silica nanoparticles was investigated using thermogravimetric analysis and zeta potential. The measured size from zeta potential analysis was 411.3–493 nm and the stability of mass reduction was located at 200 °C with final amount of mass reduced ∼88% and an average polydispersity Index was 0.195–0.224.
  • Publication
    Distinct Detection of Ganoderma Boninense On Metal Oxides-Gold Nanoparticle Composite Deposited Interdigitated Electrode DNA sensor
    ( 2021-12-14)
    Thivina V.
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Adilah Ayoib
    ;
    Nordin N.K.S.
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    Oil palms suffer severe losses due to Ganoderma boninense infection that causes Basal Stem Rot (BSR). The available detection measuring the severity of BSR disease have not proved satisfactory output. Due to the influence of oil palm industry in country's economy, effective and efficient means of diagnostic measure is mandatory. Among the available diagnostic tools, biosensors were redeemed to yield the most rapid and selective results. To overcome the current issues, herein Interdigitated Electrode (IDE) electrochemical DNA biosensor to detect Ganoderma boninense was successfully designed and fabricated by thermal deposition. Lift-off photolithography fabrication process was applied followed by the surface chemical functionalization via seed deposition. Zinc Oxide (ZnO) and Titanium Dioxide (TiO2) were overlaid and the functionalized metal oxides IDE surfaces were used to detect DNA sequence complementation from Ganoderma boninense. Furthermore, gold nanoparticles were doped to increase the surface to volume ratio and enhance biocompatibility. Characterizations were made by validating the sensor's topology characteristics and electrical characteristics. From the results recorded, it has been justified that IDE with ZnO doped with gold nanoparticles surface serves as an excellent DNA sensor for the detection of Ganoderma boninense with a remarkable current of 290 nA and 176 nA for immobilization and hybridization respectively.
  • Publication
    Harumanis Mango: Perspectives in Disease Management and Advancement using Interdigitated Electrodes (IDE) Nano-Biosensor
    ( 2020-07-09)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Uda Hashim
    ;
    Hakimi A.
    ;
    Muhammad Nur Afnan Uda
    ;
    Anuar A.
    ;
    Bakar M.A.A.
    ;
    Sulaiman M.K.
    ;
    Parmin N.A.
    The agriculture sector plays an important role in economic development in providing rural employment, uplifting rural incomes and ensuring national food security. Harumanis mango has been predominantly produced in the Perlis state of Malaysia, and it is the seasonal once-a-year. However, plant diseases became a serious issue that significantly affects both the quality and quantity of Harumanis mango production. The primary problem with Harumanis mango has experienced by farmers is a disease and the farmers are failed to complete the process of post-harvest for Harumanis mango. Further, in the case of Harumanis mango disease, the symptoms are often detected at the later stage, which makes it difficult to take any further action. Usually, the diseases are controlled by fungicides and the use of synthetic chemicals to eliminate the postharvest diseases and deterioration has their limit due to carcinogenicity, teratogenicity, environmental pollution, effects on food and other side-effects on humans. Up to date, the control of Harumanis mango disease is still challenging, although several studies have been conducted to eradicate and prevent its outbreak in the field. This overview is about Harumanis mango and the prospect in preventing a serious outbreak with Harumanis mango production using interdigitated electrodes (IDE) nano-biosensor.
  • Publication
    Potential of Syntesized Silica Nanoparticles (Si-NPs) using Corn Cob for Arsenic Heavy Metal Removal
    ( 2020-07-09)
    Balasubramaniam T.
    ;
    Bakar A.H.A.
    ;
    Muhammad Nur Aiman Uda
    ;
    Uda Hashim
    ;
    Parmin N.A.
    ;
    Anuar A.
    ;
    Bakar M.A.A.
    ;
    Muhammad Nur Afnan Uda
    ;
    Sulaiman M.K.
    According to recent studies, millions of people are suffering from Arsenic (As) toxicity contamination. Arsenic is one of the most toxic and carcinogenic chemical elements and its contamination in paddy field has become a chronic problem. In the agricultural sector, arsenic-contaminated water irrigation has a higher level of arsenic contamination on the top soil, which can affect the quality of crop production such as rice plants. Rice plant requires a considerable amount of water to complete its lifecycle. In Asean countries like Malaysia, Indonesia and Thailand, Arsenic intake from rice is significantly higher, as rice plants have a special ability to take up arsenic from the soil and water used for irrigation. The transfer of arsenic from soil to plant systems is a serious issue that leads to considerable human exposure. Nanotechnology application plays vital role for any field work and research. In this research, the implementation of nanotechnology approach such as silica nanoparticles are able to remove the Arsenic heavy metal contamination. This paper focused on arsenic heavy metal removal using silica nanoparticles analaysed using spectrophotometric method.