Asral Bahari Jambek
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Preferred name
Asral Bahari Jambek
Official Name
Asral , Bahari Jambek
Alternative Name
Jambek, Asral Bahari
Bahari Jambek, Asral
Jambek, Asral Bin Bahari
Main Affiliation
Scopus Author ID
57195722408
Researcher ID
AAG-2050-2021

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  • Publication
    Development of Internet of Things (IOT) Based Electronic Reader for Medical Diagnostic System
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Bahrin M.A.F.
    This paper is about an experiment for performing foodborne pathogens electronic reader using wireless sensing Internet of Thing (IoT). There are limited number of electronic readers for biosensors application with wireless internet connection. This research is to overcome the problem of commercial available electronic reader based on biosensor application method that only can be perform in offline or standalone device. This paper shows a complete system on how the data from electronic reader can be collected, easily understand by user and transfer data through the wireless internet connection via platform of IoT. There are three stages that is coding modification, android application development and transmit data to cloud storage. The NodeMCU microcontroller was used as a transfer medium for transfer data to internet. The Android Studio software was used for mobile application development. While, Arduino software was used to create a programming code to upload in NodeMCU microcontroller.
  • Publication
    Nano-micro-mili Current to Mili Voltage Amplifier for Amperometric Electrical Biosensors
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Parmin N.A.
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Shaharuddin S.N.A.
    ;
    Adam H.
    Amplification of nano and mircoampere electrical signal to the detectable range is essential in the biosensor field. This research is mainly focused on design an amplifier circuit to capture and amplify three different range of current as nano, micro and mili ampere and convert it to detectable voltage range as an output signal to the processing circuit. The Proteus 8 Pro software was used to design, simulate and calibrate the amplifier circuit. Firstly, current input as mili, micro and nano current were flown through 0.1 m, 10 and 10 K resistors, respectively to convert different current inputs to the similar range in micro voltage. The MAX 4238 opamp IC was used to amplify micro voltage to mili voltage. LM 358 dual operational amplifier was used to supply virtual ground to MAX 4238 amplifier. The amplified output voltage of three different current inputs as nano, micro and mili were nearly equal to theoretical outputs.
  • Publication
    Development of Voltage Amplifier Electronic Reader for Multiplex Detection of Two Electrode Electrical Biosensors
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    Amperometric electrical biosensors have small current variations at nano to micro range. There are limited number of electrical based two electrode electronic readers for biosensors are commercial available because of the amplification and noise issues at nano to micro ampere current range. The electronic reader focused on design a voltage amplifier circuit to capture and amplify three different range of current as nano, micro and mili ampere and convert it to detectable voltage range as an output voltage signal. Current input as nano, micro and mili current were flown through 10 K , 10 and 10 m resistors, respectively to convert different current inputs to the similar range in micro voltage. Then, MAX 4238 op-amp IC was used to amplify micro voltage to mili voltage. Arduino Uno circuit was act as the process and control circuit to read the output voltage from the amplifier circuit. Arduino Uno circuit will convert analog signal to digital signal and then the output voltage value is display in the LCD screen. The Proteus 8 Pro software was used to design, simulate and calibrate the amplifier circuit and Arduino Uno circuit. While, Arduino software was used to create a programming code and to upload in Arduino Uno circuit. Start your abstract here.
  • Publication
    Evaluation and Optimization of Genomic DNA Extraction from Food Sample for Microfluidic Purpose
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Nor Azizah Parmin
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Shaharuddin S.N.A.
    Contamination of various food samples became one of the critical issues in food pathogen infection. Food pathogen can be detected by using digital polymerase chain reaction (PCR) and sequencing. These methods were reliable but consuming and take a longer time for detection. The present work describes the innovation to develop a technology to extract double-stranded deoxyribonucleic acid (dsDNA) from food samples and then denatured dsDNA into and single-strand DNA (ssDNA) for further use on the chip using microfluidic device. Microfluidic device is a lab-on-chip device that consist of microfluidic channels that provide paths for biomolecules to flow to individual point of care. DNA extraction is the process by which DNA is separated from proteins, membranes, and other cellular material contained in the cell from which it is recovered. Lysis solution is used in the process of extraction the DNA to break up the cells containing DNA from protein and other cellular materials. This extraction firstly be done in the most labour-intensive in obtaining the DNA biomolecules. Extraction methods may require an overnight incubation, may be a protocol that can be completed in minutes or a couple of hours by using a commercial kit. The disadvantages of the laboratory and commercial kit is due to time-consuming, poor cost-effectiveness, the need to use big laboratory and a complicated process which need an expertise to conduct the experiment and interpret the data. This research is proposed to design and fabricate a microfluidic device that has DNA extraction capabilities. In this research DNA extraction using a commercial kit will be used as a comparison for the quality of the result. The microfluidic device can be used in health care delivery system and will help the doctors in diagnostic process to identify disease of a patient rapidly. Other than that, the output extracted from microfluidic device will be used for DNA probe target interaction for diagnostic kit. The major advantage of microfluidic device is that it consumes less time compared to the conventional chemical methods.
  • Publication
    Aluminium interdigitated electrode with 5.0 μm gap for electrolytic scooting
    ( 2024-06)
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Tijjani Adam
    ;
    Asral Bahari Jambek
    ;
    Ismail Saad
    ;
    Nor Azizah Parmin
    ;
    Shahidah Arina Shamsuddin
    ;
    Nursakinah Abdul Karim
    ;
    G. Yashni
    ;
    Nur Hulwani Ibrahim
    ;
    N. Parimon
    ;
    M. F. H. Rani
    The goal of the research project is to design, fabricate, and characterize an extremely sensitive biosensor for use in healthcare. Using AutoCAD software, a novel IDE pattern with a 5 μm finger gap was created. Conventional photolithography and regular CMOS technology were used in the fabrication process. A 3D nano profiler, scanning electron microscopy (SEM), high-power microscopy (HPM), and low-power microscopy (LPM) were used to physically characterize the manufactured IDE. Chemical testing was done using several pH buffer solutions, and electrical validation was performed using I-V measurements. The Al IDE was produced, with a tolerance of 0.1 μm between the fabricated IDEs and the design mask. Electrical measurements verified the flawless fabrication of the IDE, and the device's repeatability was validated by the outcomes of comparable IDE samples. For each pH buffer solution, a modest additional volume of 2 μl was used to quantitatively detect slight current fluctuations in the microampere range. Through pH calibration for advanced applications in the realm of chemical sensors using an amperometric method, this research study has verified the chemical behavior of the IDE.
  • Publication
    Design 5.0 µm Gap Aluminium Interdigitated Electrode for Sensitive pH Detection
    ( 2020-07-09)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Nor Azizah Parmin
    ;
    Bakar A.H.A.
    ;
    Anuar A.
    ;
    Bakar M.A.A.
    ;
    Sulaiman M.K.
    The aim of the research study to design high sensitive biosensor for medical applications. IDE pattern was designed using AutoCAD software with 5 µm ginger gap. The fabrication process was done using a conventional photolithography process and standard CMOS process. The fabricated electrode was physically characterized using a low power microscope (LPM) and a high power microscope (HPM). The electrically validated through I-V measurements and chemically tested with different pH buffer solutions. Al IDE was well fabricated with 0.1 µm tolerance between the design mask and fabricated IDEs. Electrical measurements confirmed that IDE was well fabricated without any shortage and results of similar IDE samples were confirmed that the repeatability of the device. The extremely small current variations in nano ampere range were quantitatively detected using an extra small volume of 2 µl for different pH buffer solutions. It is confirmed that IDEs are sensitive in both alkali and hydroxyl ions medium.
      1  4
  • Publication
    Aluminium Interdigitated Electrode Based Biosensor for Specific ssDNA Target Listeria Detection
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    ;
    Bahrin M.A.F.
    Nowadays interdigitated electrode (IDE) based sensor have stimulated increasing interest in the application of biosensor filed. A large number of finger electrodes as comb structure gain high sensitivity through electrical measurements. In this paper, we have investigated Listeria bacteria detection through the electrical based IDE. Listeria monocytogenes is a food borne pathogen-based bacterium that can cause dangerous disease to human, some infection may result in death. The AutoCAD software was used to design the chrome mask of IDE sensor and the fabrication process was done using conventional photolithography method. The fabricated Al IDE morphologically analyzed using a low power microscope (LPM), a high-power microscope (HPM) and 3D profiler. Functionalization step of the Al IDE, silanization process was done using (3-Aminopropyl) triethoxysilane (APTES), immobilization process was done using carboxylic probe Listeria and Tween-20 as a blocking agent for nonspecific binding on the non-immobilized area of the biosensor surface. The biosensor was validated with complementary, non-complementary and single base mismatch ssDNA targets. Different concentration of complementary ssDNA target from 1 fM to 1 M was done for the sensitivity detection.
      2
  • Publication
    Design Low Noise Voltage Amplifier for Hand-Held Electronic Reader
    ( 2020-07-09)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    There are limited number of electrical based two type electrode electronic readers for biosensors are commercial available because of the noise issues and amplification at nano to pico ampere current range. This research is mainly focused on designing an active low pass filter circuit of electronic reader for biosensors. The entire circuits are comprised of a voltage converter circuit, active low pass filter circuit, voltage amplifier, microcontroller and display unit. The circuit capture, filter and amplify nano and pico ampere current convert it to detectable voltage range as an output signal to the processing circuit. NodeMCU was act as the process and control circuit to read the output voltage from the amplifier circuit. The signal generator will act as a replacement for the biosensor input current and oscilloscope will display the input and output signal. The Design Spark PCB software was used to design the voltage amplifier circuit. Arduino software was used to create a programming code to upload in NodeMCU microcontroller.
      2
  • Publication
    Electrical DNA Biosensor Using Aluminium Interdigitated Electrode for Salmonella Detection
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    Nowadays there are many alternative methods that have been discovered and developed for the rapid detection of foodborne pathogens that can cause food poisoning. Unfortunately, majority of them still requires improvement in sensitivity and selectivity issues to be of any practical use daily. In this research, biosensors was prepared from 5 m gap Aluminium interdigitated electrode (Al IDE) to detect Salmonella enterica typhi (S. typhi). The IDE sensors in the biosensor field is extremely interest in these days due to the high number of finger electrodes as comb structure which can gain high sensitivity through electrical measurements. S. typhi is a serious food borne pathogen, makes typhoid disease which causes many deaths annually in worldwide. Functionalization steps of the Al IDE to create biosensor was based on silanization by APTES, immobilization with carboxylic functionalized S. typhi ssDNA probes and blocking agent with tween-20 were the major functionalization steps. The functionalized steps were electrically characterized using current voltage measurements. The selectivity measurement was performed with specific target was identified electrically using complementary, non-complementary and single base mismatch ssDNA target.
      2
  • Publication
    Aluminium Interdigitated Electrode with 5.0 µm Gap for Electrolytic Scooting
    ( 2024-06-01)
    Afnan Uda M.N.
    ;
    Uda Hashim
    ;
    Asral Bahari Jambek
    ;
    Tijjani Adam
    ;
    Saad I.
    ;
    Muhammad Nur Aiman Uda
    ;
    Nor Azizah Parmin
    ;
    Shahidah Arina Shamsuddin
    ;
    Norizah Abd Karim
    ;
    Yashni G.
    ;
    Ibrahim N.H.
    ;
    Parimon N.
    ;
    Rani M.F.H.
    The goal of the research project is to design, fabricate, and characterize an extremely sensitive biosensor for use in healthcare. Using AutoCAD software, a novel IDE pattern with a 5 µm finger gap was created. Conventional photolithography and regular CMOS technology were used in the fabrication process. A 3D nano profiler, scanning electron microscopy (SEM), high-power microscopy (HPM), and low-power microscopy (LPM) were used to physically characterize the manufactured IDE. Chemical testing was done using several pH buffer solutions, and electrical validation was performed using I-V measurements. The Al IDE was produced, with a tolerance of 0.1 µm between the fabricated IDEs and the design mask. Electrical measurements verified the flawless fabrication of the IDE, and the device's repeatability was validated by the outcomes of comparable IDE samples. For each pH buffer solution, a modest additional volume of 2 µl was used to quantitatively detect slight current fluctuations in the microampere range. Through pH calibration for advanced applications in the realm of chemical sensors using an amperometric method, this research study has verified the chemical behavior of the IDE.
      1  14