Uda Hashim
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Uda Hashim
Official Name
Uda, Hashim
Alternative Name
Hashimb, U.
Hashim, Uda
Hashim, U.
Uda, Hashim
Main Affiliation
Scopus Author ID
22633937800
Researcher ID
CVC-6955-2022

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  • Publication
    Preliminary studies on antimicrobial activity of extracts from aloe vera leaf, citrus hystrix leaf, zingiber officinale and Sabah snake grass against bacillus subtilis
    ( 2018)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Nur Hulwani Ibrahim
    ;
    Mohd Khairul Rabani Hashim
    ;
    Nuradibah Mohd Amer
    ;
    Midhat Nabil Ahmad Salimi
    ;
    Tan Ewe Shen
    ;
    Ong Yee Fen
    ;
    Maisara Azad Mat Akhir
    ;
    Uda Hashim
    Herbal plants have several potential antimicrobial activities either as antifungal or antibacterial to fight against the disease and pathogen that attack the plants. The extractions of the Aloe vera leaf, Citrus hystrix leaf, Zingiber officinale rhizome and Sabah snake grass were selected in this study to fight against Bacillus subtilis. B. subtilis is a Gram-positive bacterium, rodshaped and catalase-positive that lives on decayed organic material. It is known as Gram-positive bacteria because of its thick peptidoglycan and would appear purple when subjected to Gram test. This species is commonly found in the upper layers of the soil, in meat or vegetables, in pastry, cooked meat, in bread or poultry products. The extracts of Sabah Snake Grass found to be most effective than A.vera leaf, Z. officinale, and C. hystrix against the B. subtilis.
  • Publication
    Facile electrical DNA genosensor for human papillomavirus (HPV 58) for early detection of cervical cancer
    ( 2023-07)
    F. Nadhirah Jaapar
    ;
    Nor Azizah Parmin
    ;
    Nur Hamidah Abdul Halim
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Ruslinda A. Rahim
    ;
    Sh. Nadzirah
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Wei Chern Ang
    ;
    Iffah Izzati Zakaria
    ;
    Zulida Rejali
    ;
    Amilia Afzan
    ;
    Azrul Azlan Hamzah
    ;
    Chang Fu Dee
    ;
    F. Syakirah Halim
    For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10μM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.
  • Publication
    Production and characterization of silica nanoparticles from fly ash: conversion of agro-waste into resource
    ( 2021-01-01)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Uda Hashim
    ;
    Halim N.H.
    ;
    Parmin N.A.
    ;
    Muhammad Nur Afnan Uda
    ;
    Anbu P.
    A chemical method to synthesize amorphous silica nanoparticles from the incinerated paddy straw has been introduced. The synthesis was conducted through the hydrolysis by alkaline-acidic treatments. As a result, silica particles produced with the sizes were ranging at 60–90 nm, determined by high-resolution microscopy. The crystallinity was confirmed by surface area electron diffraction. Apart from that, chemical and diffraction analyses for both rice straw ash and synthesized silica nanoparticles were conducted by X-ray diffraction and Fourier-transform infrared spectroscopy. The percentage of silica from the incinerated straw was calculated to be 28.3. The prominent surface chemical bonding on the generated silica nanoparticles was with Si–O–Si, stretch of Si–O and symmetric Si–O bonds at peaks of 1090, 471, and 780 cm−1, respectively. To confirm the impurities of the elements in the produced silica, were analyzed using X-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. The stability of silica nanoparticles was investigated using thermogravimetric analysis and zeta potential. The measured size from zeta potential analysis was 411.3–493 nm and the stability of mass reduction was located at 200 °C with final amount of mass reduced ∼88% and an average polydispersity Index was 0.195–0.224.
  • Publication
    Distinct Detection of Ganoderma Boninense On Metal Oxides-Gold Nanoparticle Composite Deposited Interdigitated Electrode DNA sensor
    ( 2021-12-14)
    Thivina V.
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Adilah Ayoib
    ;
    Nordin N.K.S.
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    Oil palms suffer severe losses due to Ganoderma boninense infection that causes Basal Stem Rot (BSR). The available detection measuring the severity of BSR disease have not proved satisfactory output. Due to the influence of oil palm industry in country's economy, effective and efficient means of diagnostic measure is mandatory. Among the available diagnostic tools, biosensors were redeemed to yield the most rapid and selective results. To overcome the current issues, herein Interdigitated Electrode (IDE) electrochemical DNA biosensor to detect Ganoderma boninense was successfully designed and fabricated by thermal deposition. Lift-off photolithography fabrication process was applied followed by the surface chemical functionalization via seed deposition. Zinc Oxide (ZnO) and Titanium Dioxide (TiO2) were overlaid and the functionalized metal oxides IDE surfaces were used to detect DNA sequence complementation from Ganoderma boninense. Furthermore, gold nanoparticles were doped to increase the surface to volume ratio and enhance biocompatibility. Characterizations were made by validating the sensor's topology characteristics and electrical characteristics. From the results recorded, it has been justified that IDE with ZnO doped with gold nanoparticles surface serves as an excellent DNA sensor for the detection of Ganoderma boninense with a remarkable current of 290 nA and 176 nA for immobilization and hybridization respectively.
  • Publication
    Harumanis Mango: Perspectives in Disease Management and Advancement using Interdigitated Electrodes (IDE) Nano-Biosensor
    ( 2020-07-09)
    Muhammad Nur Aiman Uda
    ;
    Subash Chandra Bose Gopinath
    ;
    Uda Hashim
    ;
    Hakimi A.
    ;
    Muhammad Nur Afnan Uda
    ;
    Anuar A.
    ;
    Bakar M.A.A.
    ;
    Sulaiman M.K.
    ;
    Parmin N.A.
    The agriculture sector plays an important role in economic development in providing rural employment, uplifting rural incomes and ensuring national food security. Harumanis mango has been predominantly produced in the Perlis state of Malaysia, and it is the seasonal once-a-year. However, plant diseases became a serious issue that significantly affects both the quality and quantity of Harumanis mango production. The primary problem with Harumanis mango has experienced by farmers is a disease and the farmers are failed to complete the process of post-harvest for Harumanis mango. Further, in the case of Harumanis mango disease, the symptoms are often detected at the later stage, which makes it difficult to take any further action. Usually, the diseases are controlled by fungicides and the use of synthetic chemicals to eliminate the postharvest diseases and deterioration has their limit due to carcinogenicity, teratogenicity, environmental pollution, effects on food and other side-effects on humans. Up to date, the control of Harumanis mango disease is still challenging, although several studies have been conducted to eradicate and prevent its outbreak in the field. This overview is about Harumanis mango and the prospect in preventing a serious outbreak with Harumanis mango production using interdigitated electrodes (IDE) nano-biosensor.
  • Publication
    Potential of Syntesized Silica Nanoparticles (Si-NPs) using Corn Cob for Arsenic Heavy Metal Removal
    ( 2020-07-09)
    Balasubramaniam T.
    ;
    Bakar A.H.A.
    ;
    Muhammad Nur Aiman Uda
    ;
    Uda Hashim
    ;
    Parmin N.A.
    ;
    Anuar A.
    ;
    Bakar M.A.A.
    ;
    Muhammad Nur Afnan Uda
    ;
    Sulaiman M.K.
    According to recent studies, millions of people are suffering from Arsenic (As) toxicity contamination. Arsenic is one of the most toxic and carcinogenic chemical elements and its contamination in paddy field has become a chronic problem. In the agricultural sector, arsenic-contaminated water irrigation has a higher level of arsenic contamination on the top soil, which can affect the quality of crop production such as rice plants. Rice plant requires a considerable amount of water to complete its lifecycle. In Asean countries like Malaysia, Indonesia and Thailand, Arsenic intake from rice is significantly higher, as rice plants have a special ability to take up arsenic from the soil and water used for irrigation. The transfer of arsenic from soil to plant systems is a serious issue that leads to considerable human exposure. Nanotechnology application plays vital role for any field work and research. In this research, the implementation of nanotechnology approach such as silica nanoparticles are able to remove the Arsenic heavy metal contamination. This paper focused on arsenic heavy metal removal using silica nanoparticles analaysed using spectrophotometric method.
  • Publication
    Potentials of MicroRNA in Early Detection of Ovarian Cancer by Analytical Electrical Biosensors
    ( 2022-01-01)
    Nor Azizah Parmin
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Nadzirah S.
    ;
    Salimi M.N.
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    ;
    Rozi S.K.M.
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Azan M.I.A.
    ;
    Yaakub A.R.W.
    ;
    Hamzah A.A.
    ;
    Dee C.F.
    The importance of nanotechnology in medical applications especially with biomedical sensing devices is undoubted. Several medical diagnostics have been developed by taking the advantage of nanomaterials, especially with electrical biosensors. Biosensors have been predominantly used for the quantification of different clinical biomarkers toward detection, screening, and follow-up the treatment. At present, ovarian cancer is one of the severe complications that cannot be identified until it becomes most dangerous as the advanced stage. Based on the American Cancer Society, 20% of cases involved in the detection of ovarian cancer are diagnosed at an early stage and 80% diagnosed at the later stages. The patient just has a common digestive problem and stomach ache as early symptoms and people used to ignore these symptoms. Micro ribonucleic acid (miRNA) is classified as small non-coding RNAs, their expressions change due to the association of cancer development and progression. This article reviews and discusses on the currently available strategies for the early detection of ovarian cancers using miRNA as a biomarker associated with electrical biosensors. A unique miRNA-based biomarker detections are specially highlighted with biosensor platforms to diagnose ovarian cancer.
  • Publication
    Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles
    ( 2022-10-01)
    Jaapar F.N.
    ;
    Nor Azizah Parmin
    ;
    Halim N.H.A.
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Halim F.S.
    ;
    Ruslinda A.R.
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    ;
    Nadzirah S.
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Zakaria I.I.
    The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single-stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high-end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross-validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5′ COOH-GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3′, while 5′COOH-GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3′ as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini-size diagnostic devices for cervical cancer detection.
  • Publication
    Development of Voltage Amplifier Electronic Reader for Multiplex Detection of Two Electrode Electrical Biosensors
    ( 2020-03-18)
    Muhammad Nur Afnan Uda
    ;
    Asral Bahari Jambek
    ;
    Uda Hashim
    ;
    Muhammad Nur Aiman Uda
    Amperometric electrical biosensors have small current variations at nano to micro range. There are limited number of electrical based two electrode electronic readers for biosensors are commercial available because of the amplification and noise issues at nano to micro ampere current range. The electronic reader focused on design a voltage amplifier circuit to capture and amplify three different range of current as nano, micro and mili ampere and convert it to detectable voltage range as an output voltage signal. Current input as nano, micro and mili current were flown through 10 K , 10 and 10 m resistors, respectively to convert different current inputs to the similar range in micro voltage. Then, MAX 4238 op-amp IC was used to amplify micro voltage to mili voltage. Arduino Uno circuit was act as the process and control circuit to read the output voltage from the amplifier circuit. Arduino Uno circuit will convert analog signal to digital signal and then the output voltage value is display in the LCD screen. The Proteus 8 Pro software was used to design, simulate and calibrate the amplifier circuit and Arduino Uno circuit. While, Arduino software was used to create a programming code and to upload in Arduino Uno circuit. Start your abstract here.
  • Publication
    Design and Fabrication of Multichannel PDMS Microfluidic
    ( 2021-12-14)
    Muhammad Nur Aiman Uda
    ;
    Uda Hashim
    ;
    Muhammad Nur Afnan Uda
    ;
    Nor Azizah Parmin
    ;
    Thivina V.
    Microfluidic delivers miniaturized fluidic networks for processing liquids in the microliter range. In the recent years, lab-on-chip (LOC) is become a main tool for point-of-care (POC) diagnostic especially in the medical field. In this paper, we presented a design and fabrication on multi disease analysis using single chip via delivery of fluid with the multiple transducers is the pathway of multi-channel microfluidic based LOC's. 3 in 1 nano biosensor kit was attached with the microfluidic to produce nano-biolab-on-chip (NBLOC). The multi channels microfluidic chip was designed including the micro channels, one inlet, three outlet and sensor contact area. The microfluidic chip was designed to include multiplex detection for pathogen that consists of multiple channels of simultaneous results. The LOC system was designed using Design Spark Mechanical software and PDMS was used as a medium of the microfluidic. The microfluidic mold and PDMS microfluidic morphological properties have been characterized by using low power microscope (LPM), high power microscope (HPM) and surface profiler. The LOC system physical was experimental by dropping food coloring through the inlet and collecting at the sensor contact area outlet.