Ruslinda A. Rahim
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Ruslinda A. Rahim
Official Name
Ruslinda, A. Rahim
Alternative Name
Rahim, R. A.
Ruslinda, A. Rahim
A. Rahim, Ruslinda
Rahim Ruslinda, A.
Ruslinda, Abdul Rahim
Rahim, Ruslinda Abdul
Ruslinda, A. R.
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Scopus Author ID
57194735807

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  • Publication
    Facile electrical DNA genosensor for human papillomavirus (HPV 58) for early detection of cervical cancer
    ( 2023-07)
    F. Nadhirah Jaapar
    ;
    Nor Azizah Parmin
    ;
    Nur Hamidah Abdul Halim
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Ruslinda A. Rahim
    ;
    Sh. Nadzirah
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Wei Chern Ang
    ;
    Iffah Izzati Zakaria
    ;
    Zulida Rejali
    ;
    Amilia Afzan
    ;
    Azrul Azlan Hamzah
    ;
    Chang Fu Dee
    ;
    F. Syakirah Halim
    For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10μM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.
  • Publication
    Facile Electrical DNA Genosensor for Human Papillomavirus (HPV 58) for Early Detection of Cervical Cancer
    ( 2023-07-01)
    Jaapar F.N.
    ;
    Nor Azizah Parmin
    ;
    Halim N.H.A.
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Ruslinda A. Rahim
    ;
    Nadzirah S.
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Ang W.C.
    ;
    Zakaria I.I.
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Hamzah A.A.
    ;
    Dee C.F.
    ;
    Halim F.S.
    For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10µM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.
  • Publication
    Formation of polypropylene nanocomposite joint using silicon carbide nanowhiskers as novel susceptor for microwave welding
    ( 2023-05-01)
    Foong P.Y.
    ;
    Voon Chun Hong
    ;
    Lim B.Y.
    ;
    Teh P.L.
    ;
    Mohd Afendi Rojan
    ;
    Subash Chandra Bose Gopinath
    ;
    Mohd Khairuddin Md Arshad
    ;
    Nor Azizah Parmin
    ;
    Low F.W.
    ;
    Ruslinda A. Rahim
    ;
    Uda Hashim
    Up to present, no study has reported on the use of silicon carbide nanomaterials (SiCNMs) as susceptor for microwave welding of thermoplastics. Therefore, in this study, silicon carbide nanowhiskers (SiCNWs) was attempted as the microwave susceptor for the microwave welding of polypropylene (PP). It was observed that SiCNWs are capable of absorbing microwave and converting them into heat, leading to a sharp increase in temperature until it reaches the melting point of PP substrates. The microwave welded joint is formed after the molten PP at the interface between PP substrates is cooled under pressure. The effect of microwave heating duration and solid loading of SiCNWs suspension was studied and reported. The formation mechanism of SiCNWs reinforced PP welded joint was proposed in this study. With these remarkable advantages of microwave welding and enhanced mechanical properties of the welded joint, it is believed that this study can provide a new insight into welding of thermoplastic and material processing through short-term microwave heating.
      26  1
  • Publication
    Dielectric properties and microwave absorbing properties of silicon carbide nanoparticles and silicon carbide nanowhiskers
    ( 2023-04)
    Phey Yee Foong
    ;
    Voon Chun Hong
    ;
    Lim Bee Ying
    ;
    Teh Pei Leng
    ;
    Mohd Afendi Rojan
    ;
    Subash Chandra Bose Gopinath
    ;
    Nor Azizah Parmin
    ;
    Mohd Khairuddin Md Arshad
    ;
    Yeng Seng Lee
    ;
    Ruslinda A. Rahim
    ;
    Uda Hashim
    Silicon carbide (SiC) is well known for their outstanding microwave absorbing properties. SiC nanomaterials (SiCNMs) are expected to have better microwave absorption performance due to their high specific surface area. To date, no study was reported to compare the dielectric properties and microwave absorbing properties of different type of SiCNMs. Therefore, the objective of this paper is to compare the dielectric properties and microwave absorption properties of different types of SiCNMs. In this paper, SiC nanoparticles (SiCNPs) and SiC nanowhiskers (SiCNWs) were characterised using SEM and XRD. In addition, their dielectric properties and microwave absorbing properties were measured using network analyser and transmission line theory. It was found that SiCNWs achieved higher dielectric constant and loss factor which are and εr’ =17.94 and εr″ = 2.64 compared to SiCNPs that only achieved εr’ = 2.83 and εr″ = 0.71. For microwave absorbing properties, SiCNWs and SiCNPs attained minimum reflection loss of -10.41 dB and -6.83 dB at 5.68 GHz and 17.68 GHz, respectively. The minimum reflection loss of SiCNPs and SiCNWs obtained in this study is much lower than the nanometer-SiC reported previously. These results suggested that SiCNWs can be an ideal candidate of microwave susceptors for various microwave applications
      1  13
  • Publication
    Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles
    ( 2022-10-01)
    Jaapar F.N.
    ;
    Nor Azizah Parmin
    ;
    Nur Hamidah Abdul Halim
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Halim F.S.
    ;
    Ruslinda A. Rahim
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Muhammad Nur Afnan Uda
    ;
    Nadzirah S.
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Zakaria I.I.
    The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single-stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high-end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross-validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5′ COOH-GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3′, while 5′COOH-GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3′ as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini-size diagnostic devices for cervical cancer detection.
      1  52
  • Publication
    Effect of Microwave Power and Clamping Pressure on the Microwave Welding of Polypropylene Using Silicon Carbide Nanowhiskers as Microwave Susceptor
    ( 2022-01-01)
    Foong P.Y.
    ;
    Voon Chun Hong
    ;
    Lim B.Y.
    ;
    Teh P.L.
    ;
    Mohd Afendi Rojan
    ;
    Nor Azizah Parmin
    ;
    Subash Chandra Bose Gopinath
    ;
    Mohd Khairuddin Md Arshad
    ;
    Low F.W.
    ;
    Mahalingam S.
    ;
    Manap A.
    ;
    Ruslinda A. Rahim
    ;
    Uda Hashim
    Due to their excellent dielectric properties and the rapid response to microwave irradiation, silicon carbide nanowhiskers (SiCNWs) were employed as microwave susceptor in this study to absorb microwave and locally melt the surrounding polypropylene (PP) substrates for the joining of PP substrates. Complete welded joint is formed after the melted PP was cooled and resolidified. Other than microwave susceptor, SiCNWs also acted as the nanofillers in strengthening the welded joint through the formation of SiCNWs reinforced PP nanocomposite at the interface of PP substrates. Besides, the effect of microwave power on the microwave welding of PP substrates using SiCNWs as susceptor was studied and reported. It was found that the tensile strength and modulus of elasticity of the welded joint improved as microwave power increased. However, it deteriorates the flexibility of the welded joint as high stiffness SiCNWs were incorporated deeper into the PP matrix which restricted the PP chain mobility. Aside from microwave power, clamping pressure is also critical in determining the mechanical properties of a welded joint. When compared to unclamped welded joint, the tensile strength, modulus of elasticity and flexibility of welded joint subjected to clamping pressure improved drastically. Moreover, the tensile strength of welded joint increased when the clamping pressure was increased from P1 to P3, but decreased when the clamping pressure was further increased to P4 due to the occurrence of flashing at welded joint. The formation mechanism of SiCNWs reinforced PP welded joint was also proposed in this study. Compared to conventional welding, this welding process is easy, straightforward and is able to produce welded joint with outstanding mechanical properties via precise controlling of the processing parameters. Thus, microwave welding is thought to offer an option for the joining of thermoplastics and other applications.
      28  2
  • Publication
    Designing DNA probe from human Papillomavirus (HPV) 58 in E6 region as biosensing element for development of biosensor
    ( 2024-03-21)
    Jaapar, N. Fareezah
    ;
    Nor Azizah Parmin
    ;
    Nur Hamidah Abdul Halim
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Ruslinda A. Rahim
    ;
    Nadzirah S.
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Ang W.C.
    ;
    Zakaria I.I.
    ;
    Rejali Z.
    ;
    Afzan A.
    ;
    Hamzah A.A.
    ;
    Dee C.F.
    ;
    Halim, F. Syakirah
    Globally, second leading cause of death for women is a Cervical Cancer. CC is caused by infection of Human Papillomavirus (HPV). HPV strains 16 (50.8%), 18 (17.6%), and 58 (2.6%) became the most leading strains of infection in Malaysia. Recently, a study showed that HPV 58 was rare worldwide but famous in Asia countries including Malaysia. However, detection the significance of HPV-58 in women has not been studied extensively because of rare case compared to HPV 16 and 18. HPV-58 is commonly found in East Asia, but infrequently worldwide, due to changes in the environment of viruses and humans. Detailed biological knowledge is crucial for the development of effective countermeasures, diagnostic tests, vaccines and antiviral drugs against the HPV. The oligonucleotide sequences of HPV 58 in E6 region have been analysed between 24-35 mer in order to maintain the specificity and selectivity. The percentage of similarities between the coding sequences has developed with 66.7% of GC content. The DNA probe of HPV 58 was 5'GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3' with 30 mer of oligonucleotides. The important of E6 region for developing the coding sequence as it involved in the DNA reproduction, transcription, translation regulation and transformation of HPV genome. Phylogenetic trees were then constructed by Neighbour-Joining and the Kimura 2-parameters methods, followed by an analysis of selection pressures acting on the E6/E7 genes by ebi ac uk tools.
      2  20