Ahmad Anas Nagoor Gunny
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Preferred name
Ahmad Anas Nagoor Gunny
Official Name
Ahmad Anas, Nagoor Gunny
Alternative Name
Nagoor Gunny, Ahmad Anas
Gunny, A. A.N.
Nagoor Gunny, A. A.
Ahmad Anas, N. G.
Gunny, Ahmad Anas Nagoor
Main Affiliation
Scopus Author ID
55940833700
Researcher ID
AFF-6251-2022

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  • Publication
    Utilization of Seaweed (Gracilaria sp.) Liquid as Cost-Effective Macronutrients and Micronutrients for Bioethanol Production
    ( 2023-01-01)
    Zakaria N.Z.I.
    ;
    Ahmad Anas Nagoor Gunny
    ;
    Dzahir M.I.H.M.
    ;
    Ahmad A.A.
    ;
    Ismail K.S.K.
    ;
    Mahmod S.S.
    ;
    Javed M.
    ;
    B.-Yazdi N.
    ;
    Saifuddin M.N.A.A.
    Seaweed liquid waste from filter-squeezed drying process of Gracilaria sp. causes an unpleasant odour to the environment. Although this waste can partially be used as biofertilizer and heavy metals adsorbent, a bigger portion of the waste remains unutilized thus causing a problem to the environment. The present study aimed to utilize seaweed liquid waste as a supplement in fermentation media. To achieve this aim, Saccharomyces cerevisiae as a famous bioethanol producer was used to examine the ability of seaweed liquid to supply macronutrients and micronutrients in the media to produce ethanol. Interestingly, when used as a supplement in the fermentation media to produce ethanol, the liquid boosted ethanol production three folds from 200 mg/L of 2 % glucose alone to 600 mg/L of 2 % glucose in liquid. Additionally, the liquid was able to substitute yeast extract and peptone within YPD media to produce 647.48 mg/L ethanol as compared to only 542.39 mg/L with normal YPD which contains yeast extract and peptone. For that reason, the utilization of seaweed liquid as the supplement in the medium should be considered as an alternative cost-effective media for enhancing ethanol production without the addition of other nutrients.
  • Publication
    Antifungal Activity of Essential Oil Extracted from Melaleuca alternifolia Against Pathogenic Fungi on Mango (Mangifera Indica L.) for Mango Postharvest Application
    ( 2022-01-01)
    Ahmad Anas Nagoor Gunny
    ;
    Seminai J.J.A.
    ;
    Mohd Hishamuddin Che Mat
    ;
    Ahmad A.A.
    ;
    Yusof R.
    ;
    Fadhilnor Abdullah
    An infection by pathogenic fungi is one of the major problems in post-harvest stage of mangoes. Therefore, the extraction of essential oil from Melaleuca alternifolia is being tested as a natural fungicide for controlling fungus infections of selected mangoes locally known as susu mango. The extraction was performed using Solvent-Free Microwave Assisted Extraction with a yield of 0.57% concentration. The inhibitory effect of this essential oil against isolated fungi from mango was investigated through in-vitro and in-vivo analyses. Distilled water was used as a negative control while chemical fungicide (Globus 5.5) was used as a positive control in the analyses. The mycelial growth inhibition of the extracted essential oil for poisoned food test and disc diffusion method showed 62.50 ± 0.49% and 59.70 ± 0.30%, respectively, for in-vivo experiment which used unwounded and artificially wounded mangoes. The result also demonstrated that the essential oil applied on the mangoes could decrease the disease incidence from 100% to 61.33% for up to 10 days incubation at room temperature compared to that of the control. Hence, the essential oil of Melaleuca alternifolia can act as a green fungicide and is also a promising alternative to the synthetic chemical fungicide for controlling post-harvest disease on mangoes.
  • Publication
    Generation of Microcrystalline Cellulose from Cotton Waste and its Properties
    ( 2023-01-01)
    Tan W.Y.
    ;
    Subash Chandra Bose Gopinath
    ;
    Anbu P.
    ;
    Velusamy P.
    ;
    Ahmad Anas Nagoor Gunny
    ;
    Chen Y.
    ;
    Subramaniam S.
    Microcrystalline cellulose (MCC) is a green material that has widespread applications in pharmaceuticals, food, cosmetics, and other industries because of its biocompatibility, biodegradability, hydrophilicity, and acid-insolubility. In this study, MCC was prepared from cotton waste via alkaline treatment and sulfuric acid hydrolysis. Further, the synthesized cotton-based MCC was characterized using Fourier transform infrared (FTIR), X-ray photoelectron, and energy dispersive X-ray spectroscopies. Based on these results, the major components were identified as carbon and oxygen. This finding was evidenced by the FTIR analysis, which displayed peak wavenumbers at 3446.9, 2891.1, 1649.5, 1380.1, 1061.2, and 1050 to 1150 cm-1. The surface morphology was also examined by field emission scanning electron microscopy and field emission transmission electron microscopy, which showed that the prepared MCC has a smooth surface and a consistent, rod-like shape. In addition, the MCC exhibited the typical diffraction peaks of a crystalline structure of cellulose II at 12.2°, 20°, and 22.03°, which correspond to the diffraction planes of 1-10, 110, and 020, respectively, and had a crystallinity index of 78.7%. Moreover, the prepared MCC had a diameter of 37.8 µm and exhibited good stability with a peak at-76.5 mV. Further, the cotton-based MCC exhibited high thermal stability, as revealed by the TGA.
  • Publication
    Determination of degree of acetylation (DA) for chitin in deep eutectic solvents (DES)
    ( 2022-11-18)
    Suhardy Daud
    ;
    Luqman Musa
    ;
    Farizul Hafiz Kasim
    ;
    Ahmad Anas Nagoor Gunny
    ;
    Mohd Nazry Salleh
    ;
    Nazahah Mustafa
    ;
    Mubarak N.M.
    Degree of acetylation (DA) is an important parameter to determine the quality of chitin. Apart from the assessment on the bond structure in the chitin molecule, infrared spectroscopy is one of the methods that can be used to determine the value of DA. The DA value of chitin is an important parameter because the value indicates the purity of chitin quality. Chitin acetylation is the process of addition an acetyl substitution group (-COCH3) to a chitin chain. The addition of acetyl will improve its dispersing properties and subsequently will improve the chitin adhesion properties within hydrophobic matrix in composite materials as well. In this study, Deep Eutectic Solvent (DES) was used as a medium for chitin extraction and acetylation in one single process. DES has two components namely Hydrogen Bond Donor (HBD) and Hydrogen Bond Acceptor (HBA). Betaine and choline chloride were used as HBA whilst urea was selected to be utilized as HBD. The findings showed that the quantity of extracted chitins by the DESs were 5.4609 % and 2.0020 % respectively. The DA values for the extracted chitins are 103.1699 and 83.4821. For acetylated chitin in DES betaine - urea, the DA value was increased from 103.1699 to 118.4818. The findings showed that the high quality acetylated chitin can be produced in a single process involving extraction and acetylation process by using DES as a medium.
  • Publication
    The Use of Essential Oil Embedded in Polylactic Acid/Chitosan-Based Film for Mango Post-Harvest Application against Pathogenic Fungi
    ( 2023-06-01)
    Ahmad Anas Nagoor Gunny
    ;
    Leem S.J.
    ;
    Makhtar M.M.Z.
    ;
    Zainuddin N.
    ;
    Mohd Roslim M.H.
    ;
    Raja Hashim R.H.
    ;
    Pusphanathan K.
    ;
    Siddiqui M.R.
    ;
    Alam M.
    ;
    Rafatullah M.
    Mango has a high global demand. Fruit fungal disease causes post-harvest mango and fruit losses. Conventional chemical fungicides and plastic prevent fungal diseases but they are hazardous to humans and the environment. Direct application of essential oil for post-harvest fruit control is not a cost-effective approach. The current work offers an eco-friendly alternative to controlling the post-harvest disease of fruit using a film amalgamated with oil derived from Melaleuca alternifolia. Further, this research also aimed to assess the mechanical, antioxidant, and antifungal properties of the film infused with essential oil. ASTM D882 was performed to determine the tensile strength of the film. The antioxidant reaction of the film was assessed using the DPPH assay. In vitro and in vivo tests were used to evaluate the inhibitory development of the film against pathogenic fungi, by comparing the film with different levels of essential oil together with the treatment of the control and chemical fungicide. Disk diffusion was used to evaluate mycelial growth inhibition, where the film incorporated with 1.2 wt% essential oil yielded the best results. For in vivo testing of wounded mango, the disease incidence was successfully reduced. For in vivo testing of unwounded mango to which the film incorporated with essential oil was applied, although some quality parameters such as the color index were not significantly affected, weight loss was reduced, soluble solid content was increased, and firmness was increased, compared to the control. Thus, the film incorporated with essential oil (EO) from M. alternifolia can be an environmentally friendly alternative to the conventional approach and the direct application of essential oil to control post-harvest disease in mango.
  • Publication
    Effect of hydrophobic deep eutectic oil-in-water nano coating on the quality preservation of postharvest ‘Harumanis’ mango
    ( 2024-06-20)
    Gidado M.J.
    ;
    Ahmad Anas Nagoor Gunny
    ;
    Subash Chandra Bose Gopinath
    ;
    Wongs-Aree C.
    ;
    Yusoff N.H.A.
    ;
    Ibrahim R.
    ;
    Laboh R.
    ;
    Ali A.
    A hydrophobic deep eutectic oil-in-water nanoemulsion (HyDEN) was developed as a potential edible coating for preserving 'Harumanis' mango. Preventing water loss in mango during postharvest handling and storage is crucial to maintain their quality and market value. Mango with high respiration rates during storage tend to have shorter shelf life due to issues like softening and over-ripening. Edible coating materials such as lipids, polysaccharides, and proteins have setbacks such as poor water vapor barrier and potential contamination which affect their performance in preserving fruit quality. In this study, a HyDEN coating was prepared to preserve 'Harumanis' mango. The HyDEN coating exhibited good stability, with a high zeta potential value of −42.02 ± 0.12 mV, a robust antioxidant property of 97.89 ± 0.14%, and relatively low mean droplet sizes averaging 210.04 ± 0.89 nm. The application of HyDEN coating effectively extended the shelf life of 'Harumanis' mango. Additionally, it slowed down the ripening process, preserving the fruit's quality in terms of weight loss, firmness, total soluble solids, colour, titratable acidity, pH, radical scavenging rate, browning index, total phenolic content, total flavonoid content and sensory attributes after 20 days of storage, whereas the control fruit began to overripe and deteriorate after 10 days. This study introduces a novel delivery system for applying HyDEN as an edible coating for postharvest fruit preservation.
  • Publication
    Whole genome sequence of moderate halophilic marine bacterium Marinobacter litoralis SW-45: Abundance of non-coding RNAs
    ( 2019-07-15)
    Musa H.
    ;
    Farizul Hafiz Kasim
    ;
    Ahmad Anas Nagoor Gunny
    ;
    Subash Chandra Bose Gopinath
    ;
    Chinni S.V.
    ;
    Ahmad M.A.
    A report on the de novo Whole Genome Sequence (WGS)of Marinobacter litoralis SW-45, a moderately salt-tolerant bacterium isolated from the seawater in Malaysia is presented. The strain has a genome size of 3.45 Mb and is capable of producing halophilic lipase, protease and esterase enzymes. Computational prediction of non-coding RNA (ncRNA)genes in M. litoralis SW-45 was performed using standalone software known as the non-coding RNA characterization (nocoRNAc). In addition, a phylogenetic tree showing the evolutionary relationship between the strain and other members of the genus Marinobacter was constructed using 16SrRNA sequence information. A total of 385 ncRNA transcripts, 1124 terminator region, and 2350 Stress Induced Duplex Destabilization sites were predicted. The current WGS shotgun project has provided the relevant genetic information that may be useful for the strain's improvement studies. This manuscript gives the first description of M. litoralis with a complete genome.
  • Publication
    Deep Eutectic Solvent-Assisted Synthesis of Nanocrystalline Cellulose Adsorbent for Silver Nitrate Removal
    ( 2023-01-01)
    Ahmad Anas Nagoor Gunny
    ;
    Aminuddin N.H.
    ;
    Azalina Mohamed Nasir
    ;
    Hashim R.H.R.
    ;
    Jalil M.F.A.
    ;
    Pakir M.A.A.S.
    ;
    Mohamed Mydin Hj M.Abdul Kader
    ;
    Rahman A.
    This study introduces application of deep eutectic solvent-assisted of nanocrystalline cellulose adsorbent for silver nitrate removal. The study aims to offer new green and low energy method in producing nano crystalline cellulose (NCC). NCC from microcrystalline cellulose (MCC) was extracted by using synthesized acidic DES. NCC fabricated was found to have a capability to act as a biosorbent for removal of silver nitrate (AgNO3). The adsorption kinetics and isotherm of silver nitrate on NCC were studied by varies initial concentration of silver nitrate and contact time. The experimental data were analyzed by using Langmuir and Freundlich models of adsorption. The adsorption isotherm data were fitted well to Freundlich adsorption isotherm. The biosorption kinetics data obtained at different time have been analyzed using pseudo-first order and pseudo-second order equation. The experimental data fitted well to pseudo-second order kinetics model. Application of novel and green acidic DES for production of NCC from lignocellulose will contribute to a new paradigm in a green adsorption study.
  • Publication
    Inhibition of Aspergillus flavus by Momordica charantia Ethanolic Leaves Extract for Mushroom Protection
    ( 2023-06-09)
    Noor Hasyierah Mohd Salleh
    ;
    Zulkarnay Zakaria
    ;
    Aziz N.H.
    ;
    Ahmad Anas Nagoor Gunny
    ;
    Alina Rahayu Mohamed
    ;
    Munusamy T.
    Pleurotus ostreatus, better known as oyster mushroom is one of the world’s widely cultivated mushroom. Infection from pathogenic fungi is one of the major reasons causing losses of mushroom during cultivation. The objective of this study is to investigate the antifungal property of Momordica charantia leaves extract against Aspergillus flavus for mushroom protection. The inhibitory effect of M. charantia leaves extract against A. flavus isolated from infected P. ostreatus mushroom bag was investigated using pour plate technique. Inhibition of A. flavus mycelial growth was the highest at extract concentration of 9.64 v/v %, temperature 26℃ and incubation time of 96 hours with inhibitory value of 70.58 ± 0.00 %, 78.4 ± 0.00 % and 63.33 ± 2.89 % respectively. Structural morphology of the mycelia grown on the treated plate also showed morphological alteration compared to the control plate. Hence, Momordica charantia leaves extract may serves as a promising fungicides alternative to chemical use for controlling invasion of A. flavus on oyster mushroom.
  • Publication
    Studies on the third generation of Ionic liquids-Halophillic Cellulase system for Lignocellulose hydrolysis
    ( 2015)
    Ahmad Anas Nagoor Gunny
    The conversion of lignocellulosic biomass into value-added products requires pretreatment, hydrolysis (saccharification) and the conversion of simple sugar into end products. The limiting factor of these three processes lies in the pre-treatment steps. Conventional pre-treatment methods normally use strong acids or alkali which pose environmental problems. Recently pre-treatment using the third generation of Ionic Liquids (ILs), also known as Deep Eutectic Solvents (DESs) has been considered green and cost-effective. However, the main drawback of DESs pre-treatment is that it is not fully compatible with the enzyme required in saccharification. This compatibility issue was addressed in the present study by producing a salt tolerant enzyme (halophilic enzyme) and formulating low viscosity, thermal stable DES. The halophilic enzyme was produced from a newly isolated halophilic fungus, identified as Aspergillus terreus UniMAP AA-6; DESs were synthesized and screened using choline chloride as a hydrogen bond acceptor and selected carboxylic acids and polyol alcohols as hydrogen bond donors. The compatibility between halophilic cellulase and DESs was evaluated by monitoring the stability of halophilic cellulase in the presence of various concentrations of commercial ILs and DESs. The applicability of the DES-cellulase system for lignocelluloses hydrolysis was evaluated based on glucose production, energy consumption and kinetic performance. It was found that halophilic cellulase showed higher stability in the presence of 10% (v/v) ILs and also was stable and retained 90 % of its original activity in the presence of 10% (v/v) DESs. The DESs- Cellulase system exhibited higher glucose percentage enhancement and lower energy consumption as compared to diluted alkali system, while in terms of kinetic performance, DES exhibited good kinetic performance, which reflects the ability of DESs to serve as good saccharification media for the DESs-cellulase system. Finally, with regard to glucose production, rice husk treated with DESs-halophilic cellulase system were as good as the typical IL pre-treatment method but with extra benefits in terms of cost and environmental aspects. These findings have demonstrated a better approach for the in situ saccharification of DESs pre-treated lignocelluloses.