Ruslinda A. Rahim
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Preferred name
Ruslinda A. Rahim
Official Name
Ruslinda, A. Rahim
Alternative Name
Rahim, R. A.
Ruslinda, A. Rahim
A. Rahim, Ruslinda
Rahim Ruslinda, A.
Ruslinda, Abdul Rahim
Rahim, Ruslinda Abdul
Ruslinda, A. R.
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Scopus Author ID
57194735807

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  • Publication
    Dielectrophoresis as an adjunctive technique for fibroblast cell migration to enhance wound closure
    ( 2024-06)
    Nur Nasyifa Mohd Maidin
    ;
    Revathy Deivasigamani
    ;
    Muhamad Ramdzan Buyong
    ;
    Mohd Ambri Mohamed
    ;
    Ruslinda A. Rahim
    This study reports on DEP-based simulation and experimental validation of polystyrene (PS) beads and fibroblast cells for primary skin cell migration for enhancing wound closure. MyDEP software was used to calculate the numerical simulation of the Clausius-Mossotti factor (CMF). In order to examine particle trajectories based on input frequencies, the finite element technique (FEM) is used. The trajectories of PS beads and fibroblast cells were experimentally assessed to verify the impact of frequency applied on the polarisation of PS beads and fibroblast cells. The outcome illustrated the potential of employing FDEP to move particles and cells to regions of high and low electric field. Fibroblast cells exhibit negative dielectrophoresis (NDEP) at a broad range of frequencies. Thus, FDEP can be utilised for frequency optimisation to enhance wound closure.
  • Publication
    Facile electrical DNA genosensor for human papillomavirus (HPV 58) for early detection of cervical cancer
    ( 2023-07)
    F. Nadhirah Jaapar
    ;
    Nor Azizah Parmin
    ;
    Nur Hamidah Abdul Halim
    ;
    Uda Hashim
    ;
    Subash Chandra Bose Gopinath
    ;
    Ruslinda A. Rahim
    ;
    Sh. Nadzirah
    ;
    Voon Chun Hong
    ;
    Muhammad Nur Aiman Uda
    ;
    Wei Chern Ang
    ;
    Iffah Izzati Zakaria
    ;
    Zulida Rejali
    ;
    Amilia Afzan
    ;
    Azrul Azlan Hamzah
    ;
    Chang Fu Dee
    ;
    F. Syakirah Halim
    For decades, a Pap smear test has been applied as a conventional method in detecting Human Papillomavirus caused cervical cancer. False-positive results were also recorded while using it as conventional method. Current biosensor such as Hybrid (II) Capture resulted in higher time consumption and cost. s Meanwhile, in this study we provided facile, mini, rapid, highly sensitive, eco-friendly, and cost-effective sensing system focusing on HPV strain 58 (HPV58) in a nano-size lab-on-chip technology genosensor. 30-mer of virus ssDNA designed and analyzed as a probe via bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST) and ClustalW. Nanotechnology-developed colloidal Gold-nanoparticles (AuNPs) are used in the biosensor fabrication to produce high stability and electron efficient transmission during electrical measurement. AuNPs-APTES modified on active sites of IDEs, followed by immobilization of specific probe ssDNA for HPV 58. Hydrogen binding during hybridization with its target produce electrical signals measured by KEITHLEY 2450 (Source Meter). The genosensor validated with different types of targets such as complimentary, non-complementary and single mismatch oligonucleotides. The serial dilution of target concentration has been experimented triplicate (n=3) range from 1fM to 10μM. The slope of calibration curve resulted 2.389E-0 AM-1 with regression coefficient (R2) = 0.97535.
  • Publication
    Ultrasensitive and highly selective graphene-based field-effect transistor biosensor for anti-diuretic hormone detection
    ( 2020-05-01)
    Selvarajan, Reena Sri
    ;
    Ruslinda A. Rahim
    ;
    Burhanuddin Yeop Majlis
    ;
    Subash Chandra Bose Gopinath
    ;
    Azrul Azlan Hamzah
    Nephrogenic diabetes insipidus (NDI), which can be congenital or acquired, results from the failure of the kidney to respond to the anti-diuretic hormone (ADH). This will lead to excessive water loss from the body in the form of urine. The kidney, therefore, has a crucial role in maintaining water balance and it is vital to restore this function in an artificial kidney. Herein, an ultrasensitive and highly selective aptameric graphene-based field-effect transistor (GFET) sensor for ADH detection was developed by directly immobilizing ADH-specific aptamer on a surface-modified suspended graphene channel. This direct immobilization of aptamer on the graphene surface is an attempt to mimic the functionality of collecting tube V2 receptors in the ADH biosensor. This aptamer was then used as a probe to capture ADH peptide at the sensing area which leads to changes in the concentration of charge carriers in the graphene channel. The biosensor shows a significant increment in the relative change of current ratio from 5.76 to 22.60 with the increase of ADH concentration ranging from 10 ag/mL to 1 pg/mL. The ADH biosensor thus exhibits a sensitivity of 50.00 µA·(g/mL)−1 with a limit of detection as low as 3.55 ag/mL. In specificity analysis, the ADH biosensor demonstrated a higher current value which is 338.64 µA for ADH-spiked in phosphate-buffered saline (PBS) and 557.89 µA for ADH-spiked in human serum in comparison with other biomolecules tested. This experimental evidence shows that the ADH biosensor is ultrasensitive and highly selective towards ADH in PBS buffer and ADH-spiked in human serum.
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