Publications 2019

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https://hdl.handle.net/20.500.14170/3709

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Now showing 1 - 5 of 703
  • Publication
    Whole genome sequence of moderate halophilic marine bacterium Marinobacter litoralis SW-45: Abundance of non-coding RNAs
    ( 2019-07-15)
    Musa H.
    ;
    Farizul Hafiz Kasim
    ;
    Ahmad Anas Nagoor Gunny
    ;
    Subash Chandra Bose Gopinath
    ;
    Chinni S.V.
    ;
    Ahmad M.A.
    A report on the de novo Whole Genome Sequence (WGS)of Marinobacter litoralis SW-45, a moderately salt-tolerant bacterium isolated from the seawater in Malaysia is presented. The strain has a genome size of 3.45 Mb and is capable of producing halophilic lipase, protease and esterase enzymes. Computational prediction of non-coding RNA (ncRNA)genes in M. litoralis SW-45 was performed using standalone software known as the non-coding RNA characterization (nocoRNAc). In addition, a phylogenetic tree showing the evolutionary relationship between the strain and other members of the genus Marinobacter was constructed using 16SrRNA sequence information. A total of 385 ncRNA transcripts, 1124 terminator region, and 2350 Stress Induced Duplex Destabilization sites were predicted. The current WGS shotgun project has provided the relevant genetic information that may be useful for the strain's improvement studies. This manuscript gives the first description of M. litoralis with a complete genome.
  • Publication
    Detection of prostate cancer’s antigen in Sub-pico range of concentration using the faradaic-mode electrochemical impedance spectroscopy
    ( 2019-07-01)
    Ibau C.
    ;
    Md Arshad M.K.
    ;
    Gopinath S.C.B.
    ;
    Md Nor M.N.
    ;
    Rizuan N.D.
    A biosensing platform for detection of the prostate cancer’s ‘gold standard’ marker, the prostate-specific antigen (PSA) is reported. The developed assay combines the application of gold interdigitated microelectrode as the sensing device, together with the electrochemical impedance spectroscopy (EIS) in Faradaic mode of detection for a highly sensitive quantification of PSA binding on the sensor’s surface. The binding test revealed the developed assay’s capability in detecting the target in sub-pico range of concentration of 10 ng/ml down to 100 pg/ml of PSA in 100 mM phosphate buffered saline (PBS) solution (pH 7.4), covering the clinically accepted PSA threshold value for the ‘screening’ of prostate cancer at 4 ng/ml of PSA.
  • Publication
    The impact of high-k dielectric layers for SINW-FET biosensor performance improvement
    ( 2019-07-01)
    Shun M.W.
    ;
    Mohamad Fathil M.F.
    ;
    Md Arshad M.K.
    ;
    Md Nor M.N.
    ;
    Rahim R.A.
    ;
    Gopinath S.C.B.
    This paper demonstrated the application of several dielectric materials with different dielectric constant (k) (i.e. silicon dioxide (SiO2), aluminium oxide (Al2O3), and hafnium oxide (HfO2) with k values of 3.9, 9.0, and 25.0, respectively) on the silicon nanowire (SiNW) surface in order to study their effect on the performance of SiNW field-effect transistor (SiNW-FET) biosensor through simulation tool. The biosensor structure was designed and simulated in Silvaco ATLAS. Different interface charge (QF) values representing actual target biomolecules were applied onto different high-k dielectric materials surface covering the SiNW channel between drain and source as transducer for biomolecule interaction. With increase of k values exhibited by different high-k dielectric materials, the output drain currents (ID) of the biosensor were increased during detection of interface charges, which were applied on the surface of dielectric layers. Therefore, the biosensor’s performance toward detection of interface charges was improved especially when HfO2 was used as dielectric layer for the biosensor with device sensitivity and limit of detection (LOD) of 17.32 µA/e·cm2 and 1.82×1010 electronic charge/cm-2, respectively.
  • Publication
    Blood biomarkers diagnosis as primitive precautionary using zinc oxide (ZnO) modified interdigitated electrode (IDE)
    ( 2019-07-01)
    Letchumanan I.
    ;
    Md Arshad M.K.
    ;
    Gopinath S.C.B.
    ;
    Dalila R. N.
    ;
    Wan Amirah Basyarah Z.A.
    This paper primarily demonstrates the detection of human blood biomarkers by evaluating the binding event between antigen and antibody. An interdigitated electrode (IDE) was utilized in this work with the bandgap ~100 µm which was modified with antibody (probe) to capture available antigen. Surface analysis was done by atomic force microscope(AFM), scanning electron microscope (SEM) and 3D Nano Profilometer. The electrical measurements was done using linear sweep 0V to 2V. The sensitivity attained was 100 fM.
  • Publication
    CFD simulations on a phase change thermal energy storage integrated with conducting fins
    ( 2019-12-02)
    Abdul Razak M.F.
    ;
    Mohd Amin N.A.
    ;
    Abdul Majid M.S.
    ;
    Mohd Nasir N.F.
    ;
    Abdul Rahman M.D.T.
    ;
    Rahman A.A.
    Global warming has become a major problem that leads to an abnormal climate change crisis. For most countries around the world, there be would be a rise in the average daily temperature. Therefore, many research works have been conducted in order to overcome this problem. One of the most viable solutions is to store thermal energy for later use. This can be done by integrating phase change material (PCM) into thermal energy storage (TES) system. To counter the increasing temperature problem in the residential house, PCM can be implanted in the wall of the house, for instance. This way, PCM can go through freezing process during the night and countering the heat through the wall by melting process during day time. However, due to the thermal resistance within these systems, the inadequate heat transfer problem had always become the problem of low thermal conductivity. Therefore, the integration of conducting fins is proposed to the system to increase the overall thermal conductivity.