A novel aeration measurement system was developed to evaluate the water treatment process in a river to acquire a more comparable dissolved oxygen value even if various types of aerations are tested. The system comprises of DO sensor, water flowmeter, anemometer, PVC pipes, water pump, air compressor pump, and truck tyre tubes. The PVC pipes consisting of a main drainage hole, 5 holes for dissolve oxygen data collection, 1 hole as the location of aerator system, and connectors were designed as the major part of the system by using Computer Aided Design software. The main drainage hole (horizontal pipe) was designed to be 288.5 cm in length, while the measured holes (vertical pipes) were designed to be 45.7 cm in height. By considering a systematic approach, the designed system is hopefully able to solve measuring issue of dissolved oxygen in moving water and to provide a better evaluation of water treatment process.