Biohydrogen production by dark fermentation is one of the attracting alternatives for renewable energy in worldwide. By employing immobilized cells, hydrogen production and cell density could be improved. This study aimed to investigate the efficiency of Moringa Oleifera Seeds (MOS) immobilized cells in enhancing the biohydrogen production using repeated batch fermentation under mesophilic condition, 37°C. The efficiency of MOS as support carrier, effect of the initial pH (5.0-7.0) and performance of raw and diluted Palm Oil Mill Effluent (POME) using MOS immobilized cells were investigated using anaerobic sludge as inoculums. The cumulative hydrogen production results were fitted into a modified Gompertz equation to find the maximum hydrogen production. MOS immobilized cells was more efficient in producing hydrogen compare to suspended cells (without MOS). The optimal pH obtained using MOS immobilized cells was found to be at pH 6 using raw POME with the maximum hydrogen production (Hm) of 122 mL, the maximum hydrogen production rate (Rm) of 39.0 mL/h, and 560 ppm of hydrogen concentration.
