Molecular-imprinting assisted polydopamine-aptasensor on carbon and gold nanomaterials construct for the haemophilia B biomarker detection

The study presents a comprehensive approach for enhancing the performance of a spiral micro-interdigitated electrode (spiral-μIDE) sensor for the detection of FIX protein. Electropolymerization using dopamine resulted in a molecular-imprinted polymer (polyDOP-μIDE-MIP) layer, which encapsulated the aptamer-FIX complex and was later leached to create cavities. Cyclic and linear-sweep voltammetry techniques were utilized for the MIP development and rebinding assessment. Linear sweep voltammetry demonstrated a linear relationship between FIX concentration and peak current reduction, with a limit of detection (LOD) of 0.250 picomolar. The sensor's sensitivity was determined as 2.613E-10 A.fM-1.μm-2. This work highlights the importance of nanomaterials integration, and electropolymerization in improving sensor performance. The integration of carbon and gold nanomaterials and the use of molecular imprinting contribute to the sensor's enhanced sensitivity and selective detection of FIX protein.