Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a part of the family of beta-coronaviruses inducing COVID-19 disease. COVID-19 became the most life-threatening and highly contagious viral disease compare to another disease family of coronavirus. Right now, the RT-PCR is the gold standard for the diagnostic of COVID-19. To produce successful prevention strategies, medical testing, vaccinations, and antiviral drugs against COVID-19, comprehensive biological information is critical. Conserved coding RNA sequence within the N gene region of the open reading frame in the SARS-CoV-2 genome was employed as the foundation for creating an oligonucleotide probe as it is a crucial component for the development of a biosensor to identify the virus. The study aimed to develop a DNA probe that complementary to the RNA target of the N region for SARS-CoV-2. Studies were conducted on various strains of coronavirus sequences to verify the percent of correlation as well as the region of consensus that triggers various strains of viruses. Basic local alignment search tools (BLAST) and CLUSTLW had devoted additional statistical parameters, for example, desire values (E-values) and score bits. The 30 mer DNA probe with 50.0% of GC content was developed, CTG AAG CGC TGG GGG CAA ATT GTG CAA TTT. The adequate length of the probe is between 22 and 31 mer. The complementary DNA probe was designed based on the RNA target from the N-region selection that has been identified that could be used as a biomarker probe to produce a biosensor that can be implemented to the clinical and environmental diagnosis of COVID-19.
