Muhammad Mahyiddin Ramli
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Preferred name
Muhammad Mahyiddin Ramli
Official Name
Muhammad Mahyiddin, Ramli
Alternative Name
Ramli, Muhammad Mahyiddin
Ramli, M. Muhammad
Ramli, M. M.
Ramli, M.
Ramli, Muhammad Mahyidin
Mahyiddin Ramli, Mohd
Ramli, Muhammad M.
Main Affiliation
Scopus Author ID
55891565000
Researcher ID
AAH-1285-2021

Research Output
Now showing 1 - 7 of 7
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Binding dynamics and conformational stability of graphene-based nanomaterials with Mutant LOX-1: Insights from molecular docking and dynamics simulations in atherosclerosis

2024-12 , Farizah Hanim Lat , Ahmad Naqib Shuid , Mohd Yusmaidie Aziz , Muhammad Mahyiddin Ramli , Rafeezul Mohamed

Oxidized low-density lipoprotein (oxLDL) is a critical factor in endothelial dysfunction and serves as an important biomarker for oxidative stress. Recent research has focused on lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), a receptor for oxLDL that plays a significant role in atherosclerosis progression. Mutant LOX-1 may show changes in its binding affinity for oxLDL, potentially leading to variations in oxLDL uptake and foam cell formation. Our previous investigation into graphene-based nanomaterials and their interactions with atherosclerosis-related proteins, including LOX-1, provided important insights into their binding characteristics. In this study, we delve deeper into the binding dynamics between graphene-based nanomaterials and mutant LOX-1, aiming to clarify their implications for atherosclerotic development. Using molecular docking techniques with AutodockVina and active site predictions from P2Rank, we evaluated the binding affinities of graphene, graphene oxide (GO), and reduced graphene oxide (rGO) to mutant LOX-1. Notably, all docking scores were below -5 kcal/mol, indicating strong interactions with the receptor. To investigate the dynamics of these interactions further, we performed molecular dynamics (MD) simulations using the CHARMM force field. Our simulations revealed significant conformational changes within the first 100 ns, particularly in the mutant LOX-1 and GO complex, which suggested improved binding stability. These results enhance our understanding of how graphene-based nanomaterials interact with mutant forms of LOX-1, offering potential avenues for targeted therapies in atherosclerosis management related to LOX-1 dysregulation.

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The Effect of GO/TiO2 Thin Film During Photodegradation of Methylene Blue Dye

2021 , Azliza Azani , Dewi Suriyani Che Halin , Mohd. Mustafa Al Bakri Abdullah , Kamrosni Abdul Razak , Mohd Fairul Sharin Abdul Razak , V Chobpattana , Mohd Arif Anuar Mohd Salleh , Muhammad Mahyiddin Ramli

Titanium (IV) isopropoxide (TTIP) was used to synthesize GO/TiO2 thin films using a sol-gel spin-coating method onto a glass substrate, undergoing an heat tretment at 350 °C. Several amounts of graphene oxide (GO) (0-20mg) were weighed into a sol solution of TiO2 to produce GO/TiO2 thin films. The thin film samples were characterized by X-ray diffraction (XRD) to analyze the samples’ phase and by scanning electron microscopy (SEM) to analyze the samples’ microstructure. Physical testing such as water contact angle (WCA) was analyzed using an optical microscope with J-Image software. In contrast, the optical band gap and photodegradation of methylene blue under sunlight irradiation of the thin film was analyzed using UV-VIS spectrophotometry. GO5 thin film sample showed low-intensity anatase phase formation, where the microstructure revealed a larger surface area with the addition of GO. WCA reveals that GO/TiO2 thin film exhibits super hydrophilic properties where the angle decreases from 37.83° to 4.11°. The optical result shows that GO has improved the absorption edges by expanding into visible regions. Moreover, due to the existence of GO 3.30 eV band gap energy of TiO2 decreases from to 3.18 eV obtained by GO5. The improved adsorption edge allows Ti3+, O2 and interstitial states to be formed in low valence states with energy underneath than in the TiO2 band gap. Therefore, the photodegradation of methylene blue (MB) dye increases from 48 % to 59 % in the GO/TiO2 thin film.

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Evaluation of GO and rGO on breast cancer cell line (MCF7) and normal breast cell line (MCF10a) for cell viability and electrical response

2018-12 , Siti S. Mat Isa , N. S. Mazlan , Muhammad Mahyiddin Ramli , L. F. A. Talip , Rafeezul Mohamed , Zul Azhar Zahid Jamal

Graphene based materials become a phenomenal in various applications including biomedical devices due to their excellent properties. Their effects towards certain diseases were broadly studies and presented. However, the work has been performed was only limited to the graphene oxide (GO) and its biocompatibility only. In this work, the interaction of GO and reduced graphene oxide (rGO) on breast cancer cell (MCF7) and normal breast cell (MCF10a) was investigated specifically on the cell viability, cell mortality and current-voltage (IV) relationship. Graphene oxide and rGO at the concentration of 100μg/mL were prepared by chemical methods. The morphology and quality of both materials were characterized using AFM and Raman Spectroscopy. The cells were treated for 24 hours and the effects of these materials on the viability and mortality of the cell were observed. The interaction between graphene-based materials and both cells significantly impact the current-voltage (IV) characteristics. The results show that GO and rGO did not affect the cell viability but only small percentage different was obtained on cell mortality. It also observed that the resistance of cell treated with rGO decreased with time for MCF7 and vice versa for MCF10a. While for GO, the resistance of cell increased with time for MCF7 and vice versa for MCF10a. These clear patterns of these interactions lead to a good input for biosensor fabrication which was aimed to be used as the early diagnosis cancer stem cell point of care (POC) device.

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Physical, mechanical and electrical properties of Chitosan/Graphene Oxide composite films for Copper Ions (Cu²+) detection

2023 , Mohammad Abdull Halim Mohd Abdull Majid , Nurul Huda Osman , Nizam Tamchek , Nurul Asyikin Ahmad Sukri , Hazeem Ikhwan Mazlan , Nurul Najiha Mazu , Adilah Idris , Josephine Ying Chyi Liew , Muhammad Mahyiddin Ramli

Copper ions are one of the metal ions that contribute significantly to water pollution and threaten the ecosystem. The threat heightened the importance of the detection and removal of the contaminants. In this study, the Chitosan/Graphene Oxide (CH/GO) composite film was synthesized at different GO ratios via a direct casting technique. The structural, mechanical and film adsorption capacities were characterized along with the electrical properties. The results revealed that adding GO into CH at a 1:5 ratio produces the highest strength and adsorption capacity. The 1:5 film was then tested for its electrical properties to see the possibility of utilizing it as part of an electrical measurement system. Various electrical parameters such as permittivity (ε′, ε″), Tanδ, bulk resistivity (Rb) and DC conductivity (σDC) were studied. Results show that the 1:5 ratio chitosan film in various Cu²+ concentrations yielded significant differences in electrical properties. The Rb and σDC gave the most significant results and can be used as Cu²+ detection parameters.

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Remazol orange dye sensitized solar cell using graphene oxide and reduced graphene oxide working electrode

2018-12 , Norhayati Sabani , Siti S. Mat isa , Muhammad Mahyiddin Ramli , N. Rosli

Dye Sensitized Solar Cell (DSSC) is said as a potential solar device which offers easy, cheaper and greener materials and preparation process. However, the efficiency of this device is still an ultimate problem and challenge. In this paper, an organic Remazol orange dye was used as the DSSC dye sensitizer which prepared with different working electrodes. The different working electrodes consist of Titanium Dioxide (TiO2) with Graphene Oxide (GO) and TiO2 with reduced Graphene Oxide (rGO). In order to analyze the adsorption characteristics of GO and rGO, the solution was tested using Ultraviolet-Visible-Near Infrared Spectrophotometry and the surface morphology of all mixed pastes was observed under Atomic Force Microscopy and Scanning Electron Microscope. Then, the device performance was tested under illumination of solar cell simulator. From overall results, the efficiency for all tested devices was quite low from expectation. For this work, the performance of TiO2-rGO DSSC at 0.138% is 84.7% higher compared to the TiO2-GO DSSC which was 0.021%. This result was obtained when the working electrode and dye less exposed to the light during dye preparation process at 24 hours soaking time.

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The effect of polyethylene glycol addition on wettability and optical properties of GO/TiO2 thin film

2021 , Azliza Azani , Dewi Suriyani Che Halin , Kamrosni Abdul Razak , Mohd. Mustafa Al Bakri Abdullah , Marcin Nabiałek , Muhammad Mahyiddin Ramli , Mohd Fairul Sharin Abdul Razak , Andrei Victor Sandu , Wojciech Sochacki , Tomasz Skrzypczak

Modification has been made to TiO2 thin film to improve the wettability and the absorption of light. The sol-gel spin coating method was successfully used to synthesize GO/TiO2 thin films using a titanium (IV) isopropoxide (TTIP) as a precursor. Different amounts of polyethylene glycol (PEG) (20 to 100 mg) were added into the parent sol solution to improve the optical properties and wettability of the GO/TiO2 thin film. The effect of different amounts of PEG was characterized using X-ray diffraction (XRD) for the phase composition, scanning electron microscopy (SEM) for microstructure observation, atomic force microscopy (AFM) for the surface topography, ultraviolet–visible spectrophotometry (UV-VIS) for the optical properties and wettability of the thin films by measuring the water contact angle. The XRD analysis showed the amorphous phase. The SEM and AFM images revealed that the particles were less agglomerated and surface roughness increases from 1.21 × 102 to 2.63 × 102 nm when the amount of PEG increased. The wettability analysis results show that the water contact angle of the thin film decreased to 27.52° with the increase of PEG to 80 mg which indicated that the thin film has hydrophilic properties. The optical properties also improved significantly, where the light absorbance wavelength became wider and the band gap was reduced from 3.31 to 2.82 eV with the presence of PEG.

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rGO-SWCNT hybrid for counter electrode in dye sensitized solar cell

2018-12 , M. R. Muda , Siti S. Mat Isa , Muhammad Mahyiddin Ramli

Towards platinum free counter electrode in dye sensitized solar cells, some carbon-based materials like carbon nanotubes and graphene are fully utilized due to their extraordinary properties such as high aspect ratio and conductivity. In this paper, single-walled carbon nanotubes (SWCNT), reduced graphene oxide (rGO) and hybrid structure of rGO-SWCNT spray coated counter electrodes were compared with graphite as the reference material. The morphology of these materials was analyzed with FESEM and FTIR. The DSSC performance was then measured using solar simulator. The current-voltage (I-V) characteristics show that the rGO-SWCNT counter electrode result the excellent performance with efficiency of ~1.86 %, 95.2 % better than graphite. This higher performance was attributed by the complete oxygen-functional group removal during reduction process which offers large active surface area for electro-photocatalytic activity, higher conductivity, better structure and properties compared to the individual forms.

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