Now showing 1 - 8 of 8
  • Publication
    Antifungal Activity of Essential Oil Extracted from Melaleuca alternifolia Against Pathogenic Fungi on Mango (Mangifera Indica L.) for Mango Postharvest Application
    ( 2022-01-01) ;
    Seminai J.J.A.
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    Ahmad A.A.
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    Yusof R.
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    An infection by pathogenic fungi is one of the major problems in post-harvest stage of mangoes. Therefore, the extraction of essential oil from Melaleuca alternifolia is being tested as a natural fungicide for controlling fungus infections of selected mangoes locally known as susu mango. The extraction was performed using Solvent-Free Microwave Assisted Extraction with a yield of 0.57% concentration. The inhibitory effect of this essential oil against isolated fungi from mango was investigated through in-vitro and in-vivo analyses. Distilled water was used as a negative control while chemical fungicide (Globus 5.5) was used as a positive control in the analyses. The mycelial growth inhibition of the extracted essential oil for poisoned food test and disc diffusion method showed 62.50 ± 0.49% and 59.70 ± 0.30%, respectively, for in-vivo experiment which used unwounded and artificially wounded mangoes. The result also demonstrated that the essential oil applied on the mangoes could decrease the disease incidence from 100% to 61.33% for up to 10 days incubation at room temperature compared to that of the control. Hence, the essential oil of Melaleuca alternifolia can act as a green fungicide and is also a promising alternative to the synthetic chemical fungicide for controlling post-harvest disease on mangoes.
  • Publication
    Effect of hydrophobic deep eutectic oil-in-water nano coating on the quality preservation of postharvest ‘Harumanis’ mango
    ( 2024-06-20)
    Gidado M.J.
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    ; ;
    Wongs-Aree C.
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    Yusoff N.H.A.
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    Ibrahim R.
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    Laboh R.
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    Ali A.
    A hydrophobic deep eutectic oil-in-water nanoemulsion (HyDEN) was developed as a potential edible coating for preserving 'Harumanis' mango. Preventing water loss in mango during postharvest handling and storage is crucial to maintain their quality and market value. Mango with high respiration rates during storage tend to have shorter shelf life due to issues like softening and over-ripening. Edible coating materials such as lipids, polysaccharides, and proteins have setbacks such as poor water vapor barrier and potential contamination which affect their performance in preserving fruit quality. In this study, a HyDEN coating was prepared to preserve 'Harumanis' mango. The HyDEN coating exhibited good stability, with a high zeta potential value of −42.02 ± 0.12 mV, a robust antioxidant property of 97.89 ± 0.14%, and relatively low mean droplet sizes averaging 210.04 ± 0.89 nm. The application of HyDEN coating effectively extended the shelf life of 'Harumanis' mango. Additionally, it slowed down the ripening process, preserving the fruit's quality in terms of weight loss, firmness, total soluble solids, colour, titratable acidity, pH, radical scavenging rate, browning index, total phenolic content, total flavonoid content and sensory attributes after 20 days of storage, whereas the control fruit began to overripe and deteriorate after 10 days. This study introduces a novel delivery system for applying HyDEN as an edible coating for postharvest fruit preservation.
  • Publication
    Deep Eutectic Solvent-Assisted Synthesis of Nanocrystalline Cellulose Adsorbent for Silver Nitrate Removal
    ( 2023-01-01) ;
    Aminuddin N.H.
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    Hashim R.H.R.
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    Jalil M.F.A.
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    Pakir M.A.A.S.
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    Rahman A.
    This study introduces application of deep eutectic solvent-assisted of nanocrystalline cellulose adsorbent for silver nitrate removal. The study aims to offer new green and low energy method in producing nano crystalline cellulose (NCC). NCC from microcrystalline cellulose (MCC) was extracted by using synthesized acidic DES. NCC fabricated was found to have a capability to act as a biosorbent for removal of silver nitrate (AgNO3). The adsorption kinetics and isotherm of silver nitrate on NCC were studied by varies initial concentration of silver nitrate and contact time. The experimental data were analyzed by using Langmuir and Freundlich models of adsorption. The adsorption isotherm data were fitted well to Freundlich adsorption isotherm. The biosorption kinetics data obtained at different time have been analyzed using pseudo-first order and pseudo-second order equation. The experimental data fitted well to pseudo-second order kinetics model. Application of novel and green acidic DES for production of NCC from lignocellulose will contribute to a new paradigm in a green adsorption study.
  • Publication
    Effects of incorporation of essential oil into film for fruit packaging application
    ( 2021-05-24)
    Hashim R.H.R.
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    ; ;
    Kamaludin N.H.I.
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    Shamsuddin M.R.
    Biodegradable composite films with antimicrobial properties consists of three biopolymers (polysaccharides, proteins, fats), additives (plasticizers, emulsifiers), and synthetic or natural antimicrobial. Biodegradable packaging embedded in food products with antimicrobial agent such as essential oils (EOs) is an innovative green technology based on a novel approach to substitute conventional and synthetic petroleum packaging. Good packaging is necessary to prevent spoilage during postharvest stage. Introducing EOs onto the fruit packaging films strongly inhibiting bacterial and fungal activities on the fruit products and gives positive effect on the storage of the products. This review focuses on the contribution of the incorporation of EOs and biodegradable films to prolonging the shelf life of fruit products in addition to reduce the risk of growth of pathogenic bacteria on the surface of fruit products. Essential oils incorporated into film provides a new strategy and improves efficiency of fruit packaging film in enhancing quality and shelf life of post-harvest fruit products.
  • Publication
    Cellulose nanocrystals from bleached rice straw pulp: acidic deep eutectic solvent versus sulphuric acid hydrolyses
    The present work aims to investigate the feasibility of oxalic acid-choline chloride deep eutectic solvent (OA-ChCl DES), which serves as a promising green solvent that utilized in the acidic deep eutectic solvent (DES) hydrolysis. Oxalic acid-choline chloride DES cellulose nanocrystal (OA-ChCl DES CNC) was isolated from the bleached DES treated pulp (BP) through the acidic DES hydrolysis using 1:1 molar ratio of OA-ChCl DES. The functional groups, crystallinity index, morphological structure, particle size, zeta potential, thermal stability and surface chemistry of the OA-ChCl DES CNC were compared with the sulphuric acid cellulose nanocrystal (SA-CNC) that prepared via sulphuric acid hydrolysis. The findings revealed the presence of negatively charged carboxyl groups on OA-ChCl DES CNC surface after the acidic DES hydrolysis. The physicochemical analyses verified that the OA-ChCl DES CNC was in nano-sized range with polydispersity index (PdI) of 0.56, indicating slightly monodispersed nanoparticles. A stable OA-ChCl DES CNC colloidal suspension with zeta potential value of −52.1 ± 5.2 mV was obtained. The OA-ChCl DES CNC outweighed the SA-CNC in term of thermal stability (288 °C) despite having a slightly lower crystallinity index (76.7%). In fact, the OA-ChCl DES CNC with a yield of 55.1% was achieved through the acidic DES hydrolysis, suggesting that the OA-ChCl DES was capable of promoting efficient cleavage of strong hydrogen bonds in BP. Graphic abstract: [Figure not available: see fulltext.]
  • Publication
    Cellulose nanocrystal production from bleached rice straw pulp by combined alkaline and acidic deep eutectic solvents treatment: optimization by response surface methodology
    The present study aims to develop a statistical model utilizing the response surface methodology (RSM) to investigate the performance of oxalic acid-choline chloride deep eutectic solvent (OA-ChCl DES) in hydrolyzing amorphous domain of the bleached rice straw pulp (BP), while liberating the oxalic acid-choline chloride DES cellulose nanocrystal (OA-ChCl DES CNC). The process parameters, including temperature, reaction time, and BP to OA-ChCl DES mass ratio, were optimized through the RSM coupled with the face-centered central composite design (FCCCD). The mathematical models were generated, while the analysis of variance (ANOVA) was conducted to determine the most significant factors influencing the response which was measured in terms of yield. The findings revealed that the OA-ChCl DES CNC yield as high as 55.08% was attained under the acidic DES hydrolysis conditions of 79.5 Â°C, 4 h, and 1:12.64 mass ratio. This regression model enables researchers to predict the OA-ChCl DES CNC yield with respect to the influencing parameters as well as providing an insight for further scale-up process.
  • Publication
    THE EFFECT OF NANOFILLERS ON THE FUNCTIONAL PROPERTIES OF PLA AND CHITOSAN BASED FILM
    ( 2023-01-01)
    Hashim R.H.R.
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    Kamaludim N.H.I.
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    The aim of this study was to develop poly (lactic acid) and chitosan-based films and to examine the effect of cellulose nanocrystal (CNC) as nanofillers on the properties of the films. The biofilms were prepared by solvent casting method. The physical and mechanical properties of the resulting films were examined. SEM analysis showed that the surface of PLA/Cs films smoother when 1% and 2 % of CNC were added. The results for percent of water absorption of the films increased with increasing amount of CNC in PLA/Chitosan matrix. Tensile test results indicated higher TS value by incorporation of 2% of CNC. However, the PLA/Chitosan-CNC films at 3% and 4% CNC concentration exhibited a decreased TS value. PLA/Chitosan films were improved with the addition of a small amount of CNC resulting in PLA nanocomposite, which will be further evaluated for fruit packaging applications. The data obtained through this research could contribute to the establishment of a biofilms with improved the physical and mechanical properties promising significant advantages in term of longer storage life, maintaining safety, and keeping quality of a product especially in fruit packaging.
  • Publication
    Microwave-assisted solvent-free extraction of essential oil from Coleus aromaticus: anti-phytopathogenic potential for fruit post-harvesting
    ( 2021-04-01) ;
    Misnan N.M.
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    Salleh N.H.M.
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    Hashim R.H.R.
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    Mat M.H.C.
    This work evaluates the fungicidal effect of essential oil from Coleus aromaticus (C. aromaticus) by solvent-free microwave-assisted extraction with a yield of 0.54%. Fourier-transform infrared spectroscopy was utilised to identify the functional groups, which were O–H, C–O, C–H, and C=C. Gas chromatography–mass spectrometry analysis was performed to determine the primary essential oil components, namely, thymol (92.62%), thymoquinone (2.64%), creosol (1.77%), linalool (1.68%), p-Cymene-2,5-diol (0.73%), and p-Cymene (0.56%). The inhibitory effect of essential oil extracted from C. aromaticus against the isolated fungi, Aspergillus niger from mango, was investigated. The mycelial growth inhibition of the extracted essential oil by the poisoned food test and disc diffusion assay showed the reduction at 79.63 ± 1.7 and 70.45 ± 6.54%, respectively. In vivo experiment was conducted with artificially wounded and unwounded mangoes, applying the extracted essential oil to the wounded mangoes inoculated with A. niger that could decrease the disease incidence from 100 to 58.33 ± 14.43%. Meanwhile, the treatment of the extracted essential oil did not affect the quality of the mango and it also shows improvement in weight loss reduction of the mango in comparison to the chemical fungicide and untreated mango. Hence, essential oil from C. aromaticus by solvent-free microwave-assisted extraction could be used as an effective control for the fruit spoilage and potential source of fruit preservative.
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