Steven Taniselass
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Preferred name
Steven Taniselass
Official Name
Steven, Taniselass
Alternative Name
Taniselass, S.
Taniselas, S.
Taniselass, Steven
Main Affiliation
Scopus Author ID
36998664900
Researcher ID
EAA-5755-2022

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  • Publication
    Impedimetric cardiac biomarker determination in serum mediated by epoxy and hydroxyl of reduced graphene oxide on gold array microelectrodes
    ( 2021-08-01)
    Steven Taniselass
    ;
    Mohd Khairuddin Md Arshad
    ;
    Subash Chandra Bose Gopinath
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Ibau C.
    ;
    Anbu P.
    A label-free chemical bonding strategy mediated by reduced graphene oxide (rGO) basal plane functional groups has been developed for cardiac Troponin I (cTnI) detection. Four different chemical strategies on respective electrode sensing surface were precedingly examined using electrochemical impedance spectroscopy. The impedimetric assessment was carried out by sweeping frequency at the range 0.1–500 kHz perturbated at a small amplitude of AC voltage (25 mV). The chemical strategy-4 denoted as S-4 shows a significant analytical performance on cTnI detection in spiked buffer and human serum, whereby the pre-mixture of rGO and (3-Aminopropyl)triethoxysilane (APTES) creates a large number of amine sites (−NH2), which significantly enhanced the antibody immobilization without excessive functionalization. The as-fabricated immunosensor exhibited an ultra-low limit of detection of 6.3 ag mL−1 and the lowest antigen concentration measured was at 10 ag mL−1. The immunosensor showed a linear and wide range of cTnI detection (10 ag mL−1–100 ng mL−1) in human serum with a regression coefficient of 0.9716, rapid detection (5 min of binding time), and stable and highly reproducible bioelectrode response with RSD < 5%. Hence, the demonstrated S-4 strategy is highly recommended for other downstream biosensors applications. Graphical abstract: [Figure not available: see fulltext.].
  • Publication
    Battery energy storage system assessment in a designed battery controller for load leveling and peak shaving applications
    ( 2017-07-01)
    Kumuthawathe Ananda-Rao
    ;
    Rosnazri Ali
    ;
    Steven Taniselass
    This paper presents an assessment of three types of battery in a designed battery controller for a battery energy storage system (BESS) integrated with a solar photovoltaic system for load leveling and peak shaving applications. Three types of battery - lead acid, lithium ion, and nickel metal hydride - are discussed in detail and assessed in this work, focusing on small-scale integration. The controller was designed using Matlab Simulink to monitor consumer load demand, control the charging and discharging process of the BESS, and regulate while fulfilling the load demand, in addition to prolonging the BESS lifetime. From the simulation results, the nickel metal hydride battery is the best option for development of a battery controller. It achieved an average 37.45% leveled load profile and a 35.75% reduction of the load peak. However, the lead acid battery is still the dominant choice among consumers, in spite of average performance, because it has the best cost performance.
  • Publication
    Impedimetric-based biosensor for Cardiac Troponin 1 detection: sensing strategy aided by Hybrid rGO and Gold Interdigitated Electrode
    (Universiti Malaysia Perlis (UniMAP), 2021)
    Steven Taniselass
    Cardiovascular diseases (CVD) are the number one cause of death among the noncommunicable diseases globally. CVD are referred to diseases that associated with the abnormalities of blood flow in heart and often related with ‘heart attack’ condition or in clinical term as an acute myocardial infarction (AMI). Cardiac Troponin I (cTnI) biomarker is widely accepted as gold standard for AMI recognition due to its high specificity. Hence, the need for portable and highly sensitive sensor with fast detection is utmost required to diagnose AMI for fast treatments. Herein, a label-free impedimetric-based cTnI immunosensors were fabricated and their analytical performances were assessed and reported in this thesis. Reduced graphene oxide (rGO) and gold interdigitated electrode (Au-IDE) were employed to develop the immunosensors. The research work is divided into two main sections; first is to obtain a uniform deposition of rGO through single-droplet drop-casting technique and second is to fabricate the cTnI immunosensor aided by rGO. The deposition results revealed the method-3 involving post-sonication technique produces a large and self-assembled of rGO nanoflakes through single-droplet drop-casting without the use of any chemical solvent. Furthermore, the rGO modified Au-IDE devices show an excellent electron mobility, whereby the electrical conductivity was enhanced approximately ~1000-fold compared to the bare devices. Thus, the as-prepared rGO suspension was used to fabricate the immunosensors. Four different surface modification strategies on respective bioelectrodes mediated by the as-prepared rGO suspension were investigated. Electrochemical impedance spectroscopy (EIS) was performed to characterize the immunosensors by sweeping frequencies from 0.1 - 500 kHz at a small AC voltage (25 mV). Results revealed that one of the four immunosensors developed through strategy-4 shows an excellent analytical performance for the cTnI antigen detection in spiked buffer and human serum. A potential surface functionalization mediated by rGO basal plane functional groups was postulated based on XPS and FTIR analyses. The as-fabricated immunosensor showed a wide linear range (10 ag/mL – 100 ng/mL) of cTnI antigen detection in human serum with a linear regression coefficient (R2) of 0.9716 and the lowest analytes detected was 10 ag/mL, whereby it shows highly selective and sensitive trait. The immunosensor also showed a fast-detection that only required 5 minutes for probe and targets binding and stable for nine days. The bioelectrodes were highly reproducible. Hence, such an electrode sensing surface modifications strategy can be further extrapolated for later applications in various biomarker mediated disease diagnoses.