The problem in end-of-life of petroleum-based plastic management had risks wildlife and human health with rapid used in fossil reserves. Therefore, the exploration of biodegradable plastics that embraced a new eco-friendly alternative to overcome these limitations have been conducted. In this paper, the chitosan and gelatine composite film with incorporated pith and cortex of Napier grass were prepared via solution casting will be evaluated. The thermal, chemical, mechanical properties and morphological characterization of composite film had been conducted via thermo-gravimetric analysis, Fourier transforms infrared spectroscopy, tensile strength, and scanning electron microscope. The results shows that the cortex had potential to be used as reinforcement material same as the pith to broaden the use of Napier grass in industrial. Thus, further studied with additional of cellulose from pith and cortex of Napier grass in the chitosan/gelatin composite films may improve the tensile strength due to the removal of non-cellulosic component.