Finding a promising material as an alternative resource to replace the consumption of fossil fuels is necessary as the depletion of fossil fuels is increasing annually. A novel approach was explored to produce graphene from natural renewable resources. In this work, a laser scribing technique was conducted to produce large-scale graphene from Empty Fruit Bunches (EFB) of oil palm lignin biopolymer by varying the laser power. The morphology, structure, and electrical conductivity of scribed graphene were investigated at different laser power. Herein, a porous form-like structure with multilayered graphene was obtained at a laser power of 75%. The crystallite size (La) of laser power 75% was 28.4nm which shows a higher degree of graphitization was formed as laser power rises. Furthermore, the resistance was lesser at 75% laser power compared to 50% laser power concluding that 75% laser power has better electrical conductivity than low laser power. Hence, lignin-based laser scribed graphene (LSG) can be a promising and sustainable green substrate material to be utilized in supercapacitor applications.