Targeted DNA complementation on a 1,1′-carbonyldiimidazole-functionalized surface for identifying Mycobacterium tuberculosis

Xu S.; Xue Y.; Guo F.; Xu M.; Subash Chandra Bose Gopinath; Mao X.

Targeted DNA complementation on a 1,1′-carbonyldiimidazole-functionalized surface for identifying Mycobacterium tuberculosis

Journal

3 Biotech

ISSN

2190572X

Date Issued

2020-05-01

Author(s)

Xu S.

Xue Y.

Guo F.

Xu M.

Subash Chandra Bose Gopinath

Universiti Malaysia Perlis

Mao X.

DOI

10.1007/s13205-020-02216-2

Handle (URI)

https://hdl.handle.net/20.500.14170/9319

Abstract

Herein, a rapid and sensitive current–volt measurement was developed for identifying the IS6110 DNA sequence to diagnose Mycobacterium tuberculosis (TB). An aminated capture probe was immobilized on a 1,1′-carbonyldiimidazole-functionalized interdigitated electrode (IDE) silica substrate, and the target sequence was detected by complementation. It was found that all tested concentrations displayed a higher response in current changes than the control, and the limit of detection was 10 fM. The sensitivity ranged from 1 to 10 fM. The control sequences with single-, triple-mismatch and noncomplementary sequences showed great discrimination. This rapid and easy DNA detection method helps to identify M. tuberculosis for early-stage diagnosis of TB.