Gold-nanourchin seeded single-walled carbon nanotube on voltammetry sensor for diagnosing neurogenerative Parkinson's disease
Journal
Analytica Chimica Acta
ISSN
00032670
Date Issued
2020-01-15
Author(s)
Zhang R.
Shandong University, China
Wang S.
Shandong University, China
Huang X.
Shandong University, China
Yang Y.
Shandong University, China
Fan H.
Shandong University, China
Yang F.
Shandong University, China
Li J.
Shandong University, China
Dong X.
Shandong University, China
Feng S.
Shandong University, China
Anbu P.
Inha University, South Korea
Xin T.
Shandong University, China
DOI
10.1016/j.aca.2019.10.012
Handle (URI)
Abstract
α-synuclein is a predominantly expressing neuronal protein for understanding the neurodegenerative disorders. A diagnosing system with aggregated α-synuclein encoded by SNCA gene is necessary to make the precautionary treatment against Parkinson's disease (PD). Herein, gold-nanourchin conjugated anti-α-synuclein antibody was desired as the probe and seeded on single-walled carbon nanotube (SWCN) integrated interdigitated electrode (IDE). The surface morphology of SWCN-modified IDE and gold urchin-antibody conjugates were observed under FESEM, FETEM and AFM, the existing elements were confirmed. Voltammetry analysis revealed that the limit of fibril-formed α-synuclein detection was improved by 1000 folds (1 fM) with gold-nanourchin-antibody modified surface, compared to the surface with only antibody (1 pM). Validating the interaction of α-synuclein by Enzyme-linked Immunosorbent Assay was displayed the detection limit as 10 pM. IDE has a good reproducibility and a higher selectivity on α-synuclein as evidenced by the interactive analysis with the control proteins, PARK1 and DJ-1.
Funding(s)
China National Funds for Distinguished Young Scientists
Subjects
File(s)