Simple and robustic mediator free graphene nanoflake modified glassy carbon electrode (GNF/GCE) was used for the simultaneous determination of vanillin (VAN) and guaiacol (GUA) in food products like chocolate and biscuits by using an amperometric method. Defect-free graphene nanoflakes were obtained by a surfactant assisted exfoliation approach. The resulting graphene flake was characterized by FE-SEM and Raman studies. The electrocatalytic oxidation of VAN and GUA occured at +0.59 V and +0.48 V vs. Ag/AgCl at GNF/GCE, under optimum experimental condition. A well-defined peak potential window (~160 mV) for the oxidation of VAn and GUA were observed in the modified electrode. Calibration curves of VAN and GUA were attained in the ranges of 0.1 × 10–7 M to 53 × 10–6 M and 0.3 × 10–7 M to 46 × 10–6 M with correlation coefficient of 0.9966 and 0.9951, respectively. A greater sensitivity and the detection limit of VAN and GUA at 1.24 × 10–8 M and 0.98 × 10–8 M were obtained by using mediator and metal free modified electrode system in phosphate buffer medium. The present method is quite modest and robustic for the detection of VAN and GUA at a trace level. [Figure not available: see fulltext.].
