Low current output detection based electronic reader are the key factor for biosensors commercialization. Two type electrode based amperometric biosensors show low current signal output around nanoampere (pA) to microampere (μA) range. In this research, an electronic reader system with voltage converter, voltage amplifier, main control circuit and LCD display was developed to detect current output value for two type electrode amperometric biosensors in selectable ranges of nanoampere. The current source module will act as a replacement for the biosensor input current. The experiment was to verified the electrical functionality measurements of the device by comparing the accuracy of the electronic reader with Keithley 2450 source meter. The Design Spark PCB software and LTspice software were used to design the electronic reader circuit. Arduino software was used to create a programming code to upload in NodeMCU microcontroller.
