Zarina Zakaria
Thumbnail Image
Preferred name
Zarina Zakaria
Official Name
Zarina, Zakaria
Alternative Name
Zakaria, Zarina
Main Affiliation
Researcher ID
36459339400

Research Output

Filters

1
4
Reset filters

Settings
Now showing 1 - 4 of 4
  • Publication
    Protein extraction of Momordica Charantia seed assisted by ultrasound extraction
    (Springer, 2023)
    Muhamad Ikmal Sirozi
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Mohd Amin Zainal Abidin
    ;
    Norhidayah Abd Aziz
    ;
    Siti Aminah Mohd Hassan
    ;
    Zarina Zakaria
    The Momordica charantia is a tropical and subtropical plant with high nutritional value that has been implemented in traditional folk medicine as an antifungal, antimutagenic, Anthelmintic, anticancer, antifertility, and antidiabetic agent. The seeds contain rich source of antifungal protein that can be explored to be used against various fungus disease. In this study, the antifungal protein was extracted by using ultrasound assisted extraction (UAE) via one-factor-at-a-time (OFAT) technique with following the parameter sodium chloride, NaCl concentration (0.5, 1.0, 1.5, 2.0 and 2.5 M), solute ratio (0.1, 0.2, 0.3, 0.4 and 0.5 g/mL) and extraction time (4, 6, 8, 10 and 12 min). After extraction, the extract was further centrifuged and assayed by standard Bradford method before analysing the protein content at 595 nm. The result shows the optimum conditions for protein content is 6.445 mg/mL achieved after treatment with 1.5 M NaCl, 1w;10v (g/mL) in 10 min with p-value < 0.01 from each of parameter. Results showed that Momordica charantia seed had desired protein source with optimal parameter and promising antifungal bioresources with prospective applications in food and other industries.
  • Publication
    Evaluation of various lignocellulosic biomass and cereal grains as potential spawn materials for wild Schizophyllum commune cultivation
    ( 2020-12-18)
    Siti Aminah Mohd Hassan
    ;
    Sam Sung Ting
    ;
    Nur Zatul 'Iffah Zakaria
    ;
    Norhidayah Abd Aziz
    ;
    Zarina Zakaria
    ;
    Nurfatirah N.
    ;
    Azhar M.
    Rapid mycelium growth in spawn production and on growth substrate could suppress contamination, which is significant in mushroom industry. The aim of the study is to investigate the potential of lignocellulosic biomass waste as new materials alternative to common cereal grains in producing spawn for wild S. commune cultivation on rice husk, paddy straw, and rubber wood sawdust. The fastest mycelium growth among lignocellulosic biomass was found on rice husk spawn (1.27 cm/day) and 1.98 cm/day for wheat grain. The shortest duration for substrate colonization for both lignocellulosic and grain spawn is on paddy straw, followed by rice husk, and rubber wood sawdust.
      3  44
  • Publication
    Evaluation of various lignocellulosic biomass and cereal grains as potential spawn materials for wild Schizophyllum commune cultivation
    (IOP Publishing, 2020)
    Siti Aminah Mohd Hassan
    ;
    Sam Teek Ling
    ;
    N Z I Zakaria
    ;
    Norhidayah Abd Aziz
    ;
    Zarina Zakaria
    ;
    N Nurfatirah
    ;
    M Azhar
    Rapid mycelium growth in spawn production and on growth substrate could suppress contamination, which is significant in mushroom industry. The aim of the study is to investigate the potential of lignocellulosic biomass waste as new materials alternative to common cereal grains in producing spawn for wild S. commune cultivation on rice husk, paddy straw, and rubber wood sawdust. The fastest mycelium growth among lignocellulosic biomass was found on rice husk spawn (1.27 cm/day) and 1.98 cm/day for wheat grain. The shortest duration for substrate colonization for both lignocellulosic and grain spawn is on paddy straw, followed by rice husk, and rubber wood sawdust.
      6  20
  • Publication
    Influence of pH and temperature on in vitro mycelial growth performance of wild edible Schizophyllum commune of northern Malaysia
    (AIP Publishing, 2020)
    Siti Aminah Mohd Hassan
    ;
    Sam Sung Ting
    ;
    Zarina Zakaria
    The mycelial growth performance of wild isolated Schizophyllum commune of Northern Malaysia on Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA) was first compared in order to be further used in the study. A total of 7 and 13 days was required by the mycelial to fully colonized MEA and PDA in the petri dish respectively. Hence MEA was selected in investigating mycelial growth rate at different pH (5 to 10) and different temperature (20, 25, 30, and 35?). Results showed that wild S. commune growth rate was the highest (10.91±0.37mm/day) on MEA at pH 6 with incubation temperature of 30?. Biomass dry weight, pellets diameter, and final pH in Malt Extract Broth (MEB) at different pH were examined under optimal growth temperature (30?) after 14 days of liquid state fermentation. High biomass dry weight was obtained from culture incubated at pH 5 (4.84g/L) and 6 (4.87g/L) and the longest pellet diameter was obtained at pH 6 (45.6mm). There were decrements in pH value observed after end of the liquid state fermentation. It was expected that the growth data gathered in the study can be utilized later to design optimal pH and temperature conditions for artificial cultivation of S. commune.
      36  10