Siti Aminah Mohd Hassan
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Preferred name
Siti Aminah Mohd Hassan
Official Name
Siti Aminah, Mohd Hassan
Alternative Name
Mohd Hassan, S.A.
Mohd Hassan, Siti Aminah
S.A., Mohd Hassan
Main Affiliation
Scopus Author ID
57200985065
Researcher ID
CSU-9112-2022

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  • Publication
    Protein extraction of Momordica Charantia seed assisted by ultrasound extraction
    (Springer, 2023)
    Muhamad Ikmal Sirozi
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Mohd Amin Zainal Abidin
    ;
    Norhidayah Abd Aziz
    ;
    Siti Aminah Mohd Hassan
    ;
    Zarina Zakaria
    The Momordica charantia is a tropical and subtropical plant with high nutritional value that has been implemented in traditional folk medicine as an antifungal, antimutagenic, Anthelmintic, anticancer, antifertility, and antidiabetic agent. The seeds contain rich source of antifungal protein that can be explored to be used against various fungus disease. In this study, the antifungal protein was extracted by using ultrasound assisted extraction (UAE) via one-factor-at-a-time (OFAT) technique with following the parameter sodium chloride, NaCl concentration (0.5, 1.0, 1.5, 2.0 and 2.5 M), solute ratio (0.1, 0.2, 0.3, 0.4 and 0.5 g/mL) and extraction time (4, 6, 8, 10 and 12 min). After extraction, the extract was further centrifuged and assayed by standard Bradford method before analysing the protein content at 595 nm. The result shows the optimum conditions for protein content is 6.445 mg/mL achieved after treatment with 1.5 M NaCl, 1w;10v (g/mL) in 10 min with p-value < 0.01 from each of parameter. Results showed that Momordica charantia seed had desired protein source with optimal parameter and promising antifungal bioresources with prospective applications in food and other industries.
  • Publication
    Growth Optimisation of Bacillus subtilis in medium supplemented with prebiotic gum Arabic
    ( 2020-12-18)
    Norhidayah Abd Aziz
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Abu Hassan A.F.
    ;
    Amira Farzana Samat
    ;
    Adilah Anuar
    ;
    Siti Aminah Mohd Hassan
    ;
    Lian Ou Li
    Bacillus subtilis is a type of gastrointestinal bacteria that build up a healthy gut microbiota. The bacterial species has been well documented to involve in maintaining a healthy gut homeostasis in which disruption in it's microbial composition or "dysbiosis"has recently been linked to obesity. Probiotic in vivo restoration of dysbiosed microbiota have been proved successful in obese CD-1 mice model. However, prebiotic study using Gum Arabic (GA) assessing to optimise an in vitro lumen system specifically targeting to enhance the growth of B. subtilis are still lacking since this bacteria are depleted in obese individuals. Thus, this study aimed to establish the optimal growth conditions in simulated in vitro lumen system and to the best of our knowledge, this is the first in vitro study attempted to optimize the growth of B. subtilis in medium supplemented with prebiotic GA. Growth screening analysis suggested an optimal dosage of 1.0% and 0.5% glucose and GA, respectively. The highest growth rate was recorded at 0.7995 hours-1 with doubling time of 52.02 minutes with extended period of stationary phase. The optimal GA concentration and fermentation conditions were determined at 0.67%, pH 7.4 and temperature of 37 C. The validated suggested model indicates that the supplementation of GA into an optimal fermentation systems is promising to enhance the growth of gut microflora Bacillus subtilis, for restoration of a dysbiosed gut microbiota in vitro.
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