Mohammad Nuzaihan Md Nor
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Preferred name
Mohammad Nuzaihan Md Nor
Official Name
Mohammad Nuzaihan , Md Nor
Alternative Name
Nuzaihan, M. N.M.
M.Nuzaihan, M. N.
Md Nor, Mohammad Nuzaihan
Nuzaihan, M. M.
Nor, M. N.Md
Md Nor, M. Nuzaihan
Main Affiliation
Scopus Author ID
57219031365
Researcher ID
FMD-4992-2022

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  • Publication
    Top-Down Fabrication of Silicon Nanogap for Detection of Dengue Virus (DENV)
    ( 2020-01-01)
    Zulkiffli M.N.F.
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Zailan Z.
    ;
    Isa N.A.M.
    ;
    Ibau C.
    ;
    Zainol Abidin W.’.B.
    ;
    Azlan A.S.
    ;
    Mohd Khairuddin Md Arshad
    In this work, a highly sensitive Silicon nanogap biosensor was demonstrated for Deoxyribonucleic acid (DNA) detection related to Dengue virus (DENV). The Silicon nanogap was fabricated using the top–down conventional lithography approach followed by reactive ion etching (RIE) to further thin down the nanogap. The inspections of Silicon nanogap structures were carried out using the scanning electron microscope (SEM) and atomic force microscopy (AFM). The surface of the fabricated Silicon nanogap was functionalized by means of a three-steps procedure involving surface modification, immobilization and hybridization. This procedure acts as a liquid gate control to establish the electrical detection targets of the dengue virus. The electrical detection is based on the changes in the current of the sensor due to the accumulation of the negative charges by the immobilized probe and hybridized target Deoxyribonucleic acid. The limit of detection (LOD) achieved was recorded at 10 pM with a 207 nm of fabricated Silicon nanogap and its sensitivity at 1.5 × 10−10 AM−1. The demonstrated results show that the Silicon nanogap has the excellent properties for detection of dengue virus as biosensor devices.
  • Publication
    Fabrication and Characterizations of Poly-Si Nanowire Biosensor using Conventional Photolithography Technique for Detection of Dengue Virus DNA Type 2 (DENV-2)
    ( 2020-07-09)
    Shazereen Azlan A.
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Mohd Khairuddin Md Arshad
    ;
    Amirah Basyarah W.
    ;
    Ibau C.
    ;
    Mohamad Faris Mohamad Fathil
    Nowadays, nanotechnology has become a vast expanding application which can be used all across the science field such as chemistry, biology, physic, material science and engineering. In this paper, a poly-Si nanowire biosensor was fabricated by using the conventional photolithography technique. In addition, this technique is used to define the initial poly-Si with the dimension of 1 μm. After the conventional photolithography process, the photoresist undergone the development using resist developer and etched with reactive ion etching (RIE). Meanwhile, for the electrical part, it was observable that there was an increase in current when the nanowire has been hybridized with Dengue DNA type-2 (DENV-2) ranging from 10 fM - 10 μM. The morphology of the poly-Si nanowire was characterized by optical microscopy whilst electrically characterized by measuring the two-terminal current-voltage (I-V) characteristic.
  • Publication
    Faradaic electrochemical impedimetric analysis on MoS2 /Au-NPs decorated surface for C-reactive protein detection
    ( 2022-09-01)
    Dalila R N.
    ;
    Mohd Khairuddin Md Arshad
    ;
    Subash Chandra Bose Gopinath
    ;
    Ibau C.
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Azmi U.Z.M.
    ;
    Anbu P.
    Background: A label-free Faradaic electrochemical impedimetric was developed for a highly sensitive detection of C-reactive protein using a gold interdigitated microelectrode bio-sensing platform enhanced by a gold nanoparticle-decorated molybdenum disulfide (Au-NPs/MoS2) nanosheet via selected chemical linking processes. C-reactive protein (C-RP), a crystalline protein, generates by the liver and hikes when there is inflammation throughout the patients’ body. The concentrations of C-RP plasma levels tend to increase rapidly when the patient facing major injury which will lead to cardiovascular disease (CVD). Methods: The 5 µm microelectrode and gap size g-IDE with the nanostructured materials was demonstrated to increase the impedimetric detection response in Faradaic-mode electrochemical impedance spectroscopy high performance detection environment. The high surface area-to-volume ratio of the modified Au-NPs/MoS2 nanocomposite increased the probes loading onto the transducer and enhanced the impedimetric detection response of the C-RP target post-binding due to an amplified net change in the charge transfer resistance. The developed immunoassay revealed a linear detection of C-RP biomarker in a logarithmic-scale from as low as 1 fg/mL up to 1 µg/mL, and a limit of detection of 0.01 fg/mL. The sensor shows great potential as an early warning risk for cardiovascular disease at a threshold concentration value of C-RP at 1 µg/mL. Significant findings: The biosensor demonstrates an excellent discrimination against other competing proteins in serum, exhibiting the highest predilection to C-RP spiked human serum target. The sensor's reproducibility is reported within an acceptable range of relative standard deviation of 1–4% for n = 3.
  • Publication
    Nanomanipulation of Functionalized Gold Nanoparticles on GaN
    ( 2023-01-01)
    Che Seliman M.A.
    ;
    Ali Yusup N.A.
    ;
    Ahmad M.A.
    ;
    Ibau C.
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Kawarada H.
    ;
    Hassan Z.
    ;
    Packeer F.
    ;
    Falina S.
    ;
    Syamsul M.
    Gold nanoparticles (AuNPs) are known for their high surface area to volume ratio, which acts as an excellent receptor when placed in between electrodes in sensor applications. Microelectrodes with bar and needle-shaped pointed ends in two configurations, comb and castle wall, were designed to be used for the fabrication of electrodes to observe the relation between the geometry of electrodes and the dielectrophoretic behaviour of AuNPs on p-gallium nitride (p-GaN) substrates. The electrical properties were analyzed before and after the drop cast of AuNPs using current-voltage (I-V) curve method with manual probing. Resistance values of each sample were calculated under reverse bias condition. The effect of the design configurations of the electrodes on the nanomanipulation of AuNPs will be discussed.
  • Publication
    Immunosensing prostate-specific antigen: Faradaic vs non-Faradaic electrochemical impedance spectroscopy analysis on interdigitated microelectrode device
    ( 2020-11-01)
    Ibau C.
    ;
    Mohd Khairuddin Md Arshad
    ;
    Subash Chandra Bose Gopinath
    ;
    Mohammad Nuzaihan Md Nor
    ;
    Mohamad Faris Mohamad Fathil
    ;
    Shamsuddin S.A.
    This work explores Electrochemical Impedance Spectroscopy (EIS) detection for a highly-sensitive quantification of prostate-specific antigen (PSA) in Faradaic (f-EIS) and non-Faradaic modes (nf-EIS). Immobilization of monoclonal antibody specific to PSA (anti-PSA) was performed using 1-ethyl-3-dimethylaminopropylcarbodiimide hydrochloride and N-hydroxysuccinimide crosslinking agents in order to conjugate carboxylic (-COOH) terminated group of 16-Mercaptoundecanoic acid with amine (-NH3+) on anti-PSA epitope. This approach offers simple and efficient approach to form a strong, covalently bound thiol-gold (S–Au) for a reliable SAM layer formation. Studies on the topographic of pristine Au-IDE surface were performed by Scanning Electron Microscopy and Energy Dispersive X-ray Spectroscopy techniques, meanwhile a 3-dimensional optical surface profiler, Atomic Force Microscopy and X-ray Photoelectron Spectroscopy techniques were used to validate the successful functionalization steps on the sensor transducer surface. Detection of PSA in f-EIS mode was carried out by measuring the response in charge transfer resistance (Rct) and impedance change (Z), meanwhile in nf-EIS mode, the changes in device capacitance was monitored. In f-EIS mode, the sensor reveals a logarithmic detection of PSA in a range of 100 ng/ml down to 0.01 ng/ml in Phosphate Buffered Saline with a recorded sensitivity of 2.412 kΩ/log10 ([PSA] ng/ml) and the limit of detection (LOD) down to 0.01 ng/ml. The nf-EIS detection mode yields a logarithmic detection range of 5000 ng/ml down to 0.5 ng/ml, with a sensitivity of 8.570 nF/log10 ([PSA] ng/ml) and an LOD of 0.5 ng/ml. The developed bio-assay yields great device stability, specificity to PSA and repeatability of detection that would pave its way for the future development into portable lab-on-chip bio-sensing system.
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