Huzairy Hassan
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Preferred name
Huzairy Hassan
Official Name
Hassan, Huzairy
Alternative Name
Hassan, H.
Hassan, Huzairy
Main Affiliation
Scopus Author ID
55389539900
Researcher ID
GYR-0674-2022

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Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by Bacillus subtilis

2012-08-18 , Huzairy Hassan , Khairiah Abd Karim

The production of alpha-amylase by Bacillus subtilis was investigated under different cultivation conditions on two different solid substrates as the support, i.e. oil palm empty fruit bunch (OPEFB) fibers and rice straw using solid state fermentation (SSF) process. Both solid substrates were first pre-treated using NaOH to remove lignin and silica and to break down the hemicellulose structures. The influences of incubation time, incubation temperature and the additional carbon and nitrogen sources on the production of alpha-amylase by B. subtilis were investigated. After 48 h of incubation, the highest enzyme activities were obtained at 39.9 U/g and 23.5 U/g for rice straw and OPEFB fibers, respectively. The optimum temperature for alpha-amylase production was observed at 45°C for the OPEFB fibers and 55°C for the rice straw. Among the defined carbohydrates, the addition of glucose (0.02 g/g dry substrate) has significantly improved the production of alpha-amylase. Similar effect was observed when yeast extract (0.01 g/g dry substrate) was supplemented as the additional nitrogen source. Rice straw appeared to be the best solid substrate in producing alpha-amylase (276 U/g) in SSF under these optimum cultivation conditions.

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Dual-response quadratic model for optimisation of electricity generation and chlorophenol degradation by electro-degradative Bacillus subtilis in microbial fuel cell system

2022-01-01 , Huzairy Hassan , Jin B. , Dai S.

The interactions within microbial, chemical and electronic elements in microbial fuel cell (MFC) system can be crucial for its bio-electrochemical activities and overall performance. Therefore, this study explored polynomial models by response surface methodology (RSM) to better understand interactions among anode pH, cathode pH and inoculum size for optimising MFC system for generation of electricity and degradation of 2,4-dichlorophenol. A statistical central composite design by RSM was used to develop the quadratic model designs. The optimised parameters were determined and evaluated by statistical results and the best MFC systematic outcomes in terms of current generation and chlorophenol degradation. Statistical results revealed that the optimum current density of 106 mA/m2 could be achieved at anode pH 7.5, cathode pH 6.3–6.6 and 21–28% for inoculum size. Anode–cathode pHs interaction was found to positively influence the current generation through extracellular electron transfer mechanism. The phenolic degradation was found to have lower response using these three parameter interactions. Only inoculum size-cathode pH interaction appeared to be significant where the optimum predicted phenolic degradation could be attained at pH 7.6 for cathode pH and 29.6% for inoculum size.

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Optimization of chlorophenols adsorption using OPEFB biosorbent

2022-11-18 , Huzairy Hassan , Mohd Azmier Ahmad , Lim Chia Hooi , Bello Olugbenga Solomon

Palm oil industry have long been one of the most important economic drivers in the country. However, the abundance of wastes generated such as oil palm empty fruit bunch (OPEFB) and palm oil mill effluent (POME) have resulted in serious environmental issues that necessitate immediate response. This work investigates the optimization of biosorption of chlorophenols, which are commonly found in POME, using treated OPEFB biosorbent. The surface of biosorbent was modified by physical or chemical treatment. The biosorption capacity for 2,3-dichlorophenol (2,3-DCP) are higher than that of 2-chlorophenol (2-CP). Therefore, 2,3-DCP was further examined in the optimization study by Full 2k factorial design and Central Composite Design (CCD). A full 25-1 factorial design with five factors and two levels was used to screen the significant factors that influenced the biosorption process. These results were applied in the optimization using CCD with three factors (initial 2,3-DCP concentration, pH of solution and OPEFB biosorbent dosage). It is found the optimum conditions for 2,3-DCP biosorption were 200?ppm initial concentration, pH 5 and 0.15?g dosage. The maximum biosorption capacity was 13.65?mg/g. Langmuir biosorption isotherm best described the biosorption of 2,3-DCP onto OPEFB biosorbent at different initial concentrations of 2,3-DCP solution. Pseudo-second order was found to best fit the biosorption system by OPEFB biosorbent. Therefore, it is worth considering the potential use of OPEFB biomass as a biosorbent in the application of the treatment of toxic pollutants.

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Publication

Utilization of agricultural by-products for alpha-amylase production under solid state fermentation by bacillus subtilis

2012 , Huzairy Hassan , Khairiah Abd Karim

The production of alpha-amylase by Bacillus subtilis was investigated under different cultivation conditions on two different solid substrates as the support, i.e. oil palm empty fruit bunch (OPEFB) fibers and rice straw using solid state fermentation (SSF) process. Both solid substrates were first pre-treated using NaOH to remove lignin and silica and to break down the hemicellulose structures. The influences of incubation time, incubation temperature and the additional carbon and nitrogen sources on the production of alpha-amylase by B. subtilis were investigated. After 48 h of incubation, the highest enzyme activities were obtained at 39.9 U/g and 23.5 U/g for rice straw and OPEFB fibers, respectively. The optimum temperature for alpha-amylase production was observed at 45°C for the OPEFB fibers and 55°C for the rice straw. Among the defined carbohydrates, the addition of glucose (0.02 g/g dry substrate) has significantly improved the production of alpha-amylase. Similar effect was observed when yeast extract (0.01 g/g dry substrate) was supplemented as the additional nitrogen source. Rice straw appeared to be the best solid substrate in producing alpha-amylase (276 U/g) in SSF under these optimum cultivation conditions

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Current status on Microbial Fuel Cell (MFC) technology

2022-01-01 , Huzairy Hassan , Raudzah Mohd Zahir , Ahmad M.A. , Mismisuraya Meor Ahmad

Agricultural and industrial activities in Malaysia have adversely impacted environmental quality leading local researchers to mobilize their expertise and resources to protect the environment from this alarming situation. The palm oil industry, for instance, generates an abundance of wastes (such as palm oil mill effluent [POME]), which calls for effective technological tools to reduce these recalcitrant wastes from spreading further. Microbial fuel cell (MFC) is an emerging technology for wastewater treatment and electricity generation simultaneously, which appears to be the most advantageous solution for these environmental concerns. This chapter reviews the prospects of MFC research with a special emphasis on the current MFC status in Malaysia. It begins with an overview of MFC principles, electron transfer mechanisms, and bio-electrochemical performance. Following that, a review of the recent MFC developments and activities by Malaysian researchers is discussed with an emphasis on POME utilization. This chapter concludes with several resolutions to the main challenges researchers and scientists are facing regarding the scalability of MFC for electricity recovery and wastewater treatment.

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Application of OPEFB fibre based electrode in microbial fuel cell system for electricity generation and chlorophenol degradation

2021-05-24 , Raudzah Mohd Zahir , Huzairy Hassan , Ahmad Anas Nagoor Gunny

Microbial fuel cell (MFC) has emerged as one of the potential technologies for sustainable bioelectrical energy recovery and reduction of recalcitrant wastes. The MFC performance is greatly influenced by the anode materials which serve as the support for exoelectrogenic bacteria attachment. In this study, oil palm empty fruit bunch (OPEFB) is proposed as an alternative anode material prepared via a direct carbonization process using tube furnace owing to its good conductivity property. The carbonization process was conducted under nitrogen gas flow at 900℃ with a constant heating rate of 5℃/min. The anode was prepared by mixing the carbonized OPEFB with polytetrafluoroethylene (PTFE) binder. When used in MFC, the OPEFB-anode generated a maximum current density of 97.30 mA/m2, which is comparatively higher than that of the conventional carbon cloth anode (76.24 mA/m2). Our MFC system had also resulted considerable chemical oxygen demand (COD) and 2-chlorophenol reductions of 77% and 75%, respectively. This study could support future research on freely-available OPEFB materials for high performance MFC anode.

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Effect of photodegradation on chemical characteristics and enzymatic digestibility of chicken feather keratin

2024-03-21 , Aziz F. , Zarina Zakaria , Syazwani Mahmad Puzi , Huzairy Hassan

The rapid development of poultry industry has correlated with increased production of keratin containing wastes that possessed complex morphology which difficult to degrade. UV irradiation pretreatment provide an initial stage of degradation before they are further degrade using fermentation process into ready to use protein. Untreated and treated with UV light chicken feathers morphology of fibers and cuticle cells were observed using SEM. A feather protein hydrolysate was produced by keratinolytic bacteria Bacillus subtilis under submerged fermentation. Keratinolytic kinetic is study using Michealis-Menten kinetics where Km value for treated chicken feathers with UV was 22.4591 M which is smaller compare to untreated chicken feathers, 42.3065 M. The smaller Km value shows tightly binding of keratinase and chicken feathers powder. Chicken feathers that were treated with UVC has the highest protein content about 3.19 mg/mL of protein while untreated chicken feathers only contains 2.29 mg/mL of protein.

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The Optimization of Protease Enzyme Extraction from Streblus Asper (Kesinai)

2020-12-18 , Seow Yen Yi , Rozaini Abdullah , Lee Yen Fong , Zarina Zakaria , Huzairy Hassan , Sharifah Zati Hanani Syed Zuber , Mohd Ruslan, Mahfuz Affif

Protease from Streblus asper (Kesinai) is an interesting rennet substitute and yet very few studies had been conducted so far. In the present study, the leaf extract of Kesinai had been discovered to investigate the ability of this milk coagulating enzyme. The development of the optimized conditions for enzyme extraction was analyzed by using Central Composite Design (CCD). The studied factors were ratio of sample to buffer, weight of sample (g) and homogenization time (min). It was found that a 30 g of S. asper leaves sample with the ratio of the sample to buffer of 1:1 and at a mixing rate of 2 minutes established the most desirable conditions for serine proteases extraction from the S. asper leaves sample.

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Preliminary comparison of 2-chlorophenol biodegradation using microbial fuel cell and anaerobic systems

2022-04-20 , Raudzah Mohd Zahir , Huzairy Hassan , Ahmad M.A.

2-chlorophenol (2CP) is a typical contaminant found in industrial effluent that is both hazardous and persistent in the environment. The bioelectrochemical degradation of 2CP has been approved as a preferred method for removing the abrasive 2CP from wastewater. In this work, a microbial fuel cell (MFC) system inoculated with palm oil mill effluent (POME) sludge was used to degrade 2CP. The changes of morphology of the anode biofilm were observed under a light microscope and scanning electron microscope (SEM) for 2CP-fed MFC compared with the biofilm inoculated in an anaerobic chamber (AC). Maximum current density generated by the MFC was 97.30 mA/m2 while degrading 75% 2CP. Lower 2CP degradation of 60% was observed using the AC. Also, the abundance of negatively stained bacteria is reduced in the AC biofilm. This research shows that bioelectrochemical 2CP degradation is more efficient than conventional AC degradation. POME has the potential to be a high-value substrate for bacteria that can generate electricity in the MFC while also degrading harmful 2CP.

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Carbonization of sludge biomass of water treatment plant using continuous screw type conveyer pyrolyzer for methylene blue removal

2021-05-24 , Ahmad M.A. , Yusop M.F.M. , Awang S. , Yahaya N.K.E.M. , Rasyid M.A. , Huzairy Hassan

The wide and fast growth of the textile industry has become one of the contributors to the water pollution. In this study, carbonized sludge (CS) was produced as an adsorbent for removal of methylene blue (MB) dye from aqueous solution. Sludge biomass from water treatment plant as precursor was carbonized using continuous screw type conveyer pyrolyzer at different conveyer frequency of 5 Hz and 10 Hz. Batch adsorption studies were carried out by varying MB initial concentrations (25 - 300 mg/L) and contact time (0 - 24 hours). The CS5Hz sample shows the highest surface area and total pore volume of 88.73 m2/g and 0.1417 cm3/g, respectively. This sample exhibits average pore diameter of 3.84 nm, monolayer adsorption capacity of 45.68 mg/g and well develop of pores structure. Freundlich and pseudo second order models were revealed to fit the isotherm and kinetic data, respectively with film diffusion play a major role as rate limiting step.

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