The rapid development of poultry industry has correlated with increased production of keratin containing wastes that possessed complex morphology which difficult to degrade. UV irradiation pretreatment provide an initial stage of degradation before they are further degrade using fermentation process into ready to use protein. Untreated and treated with UV light chicken feathers morphology of fibers and cuticle cells were observed using SEM. A feather protein hydrolysate was produced by keratinolytic bacteria Bacillus subtilis under submerged fermentation. Keratinolytic kinetic is study using Michealis-Menten kinetics where Km value for treated chicken feathers with UV was 22.4591 M which is smaller compare to untreated chicken feathers, 42.3065 M. The smaller Km value shows tightly binding of keratinase and chicken feathers powder. Chicken feathers that were treated with UVC has the highest protein content about 3.19 mg/mL of protein while untreated chicken feathers only contains 2.29 mg/mL of protein.
