Amirul Ridzuan Abu Bakar
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Preferred name
Amirul Ridzuan Abu Bakar
Official Name
Amirul Ridzuan, Abu Bakar
Alternative Name
Bakar, Amirul Ridzuan Abu
Bakar, A. R.Abu
Abu Bakar, Amirul Ridzuan
Bakar, Amirul R.A.
Main Affiliation
Scopus Author ID
57224203273
Researcher ID
AEX-2883-2022

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Profiling of Bioactive Compounds and Bioactivity of the Kenaf (Hibiscus Cannabinus L.) Leaf Extract

2023-01-01 , Shukri S.Z.M. , Nik Muhammad Azhar Nik Daud , Amirul Ridzuan Abu Bakar , Arsad S.S. , Mohd Asraf Mohd Zainudin

The current research examines the impact of different solvents and maturity stages of the kenaf leaf (Hibiscus Cannabinus L. var. Everglade 51) on the bioactive compound and bioactivity, which specifically focused on antioxidant and antimicrobial properties. The kenaf’s leaf powder was extracted sequentially using hexane, acetone, and methanol. The total phenolic compound (TPC), flavonoid content (TFC), and free-radical scavenging activity using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay were measured using UV–Vis spectrometry. The antibacterial activity of the extract was determined using the disc diffusion method on both Gram-positive and -negative bacteria. Results show that the methanol extracts had a significant (p < 0.05) quantity of TPC and TFC compared to other extracts. Results showed that the antioxidant compound amount measured as TPC and TFC in extract increased from shoot stages into mature leaves by 11% and 24%, respectively. As expected, methanolic extract of mature leaf exerts the highest TPC and TFC value compared to that of other solvents. This also reflects the antioxidant activity among the different extract where methanolic extract showed the highest antioxidant activity with value 60.48 ± 0.24% of radical scavenging activity. Meanwhile, only the methanolic and acetone extracts demonstrated a significant (p < 0.05) inhibitory zone against Gram-negative microorganisms. Overall, mature kenaf leaf extracted with methanol and acetone solvents showed a particularly high in terms of antioxidant capacity and antimicrobial characteristics. Mature kenaf leaf was recommended for food product development due to their strong antioxidant activity and ability to inhibit Gram-negative bacteria.

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Prediction of phase equilibria in ternary diagram for system consist of Hot Compressed Water (HCW) with crude palm oil (CPO) and others minor component using thermodynamic model

2024-05-10 , Mohd Sharizan Md Sarip , Nik Muhammad Azhar Nik Daud , Mohd Asraf Mohd Zainudin , Idham Z. , Adilah Anuar , Mohd Aminudin Jamlos , Amirul Ridzuan Abu Bakar

Knowledge on phase equilibria for the separation process are important. It helps to improve the extraction efficiency through parameter optimization. Nowadays, hot compressed water extraction (HCWE) is getting the interest for the crude palm oil (CPO) extraction. Therefore, the phase equilibria of system consist of Hot compressed water (HCW) and CPO component are crucial. In this study, prediction of phase equilibria in ternary diagram for system consist of HCW with CPO and others minor component was conducted using thermodynamic model. The prediction was conducted using modified UNIFAC. CPO and other minor components namely palmitic acid which represent FFA, β-carotene, α-tocopherol, α-tocotrienol and α-tocotrieno was totally insoluble in HCW. The solubility of HCW in CPO was effect by the temperature and concentration of palmitic acid, β-carotene and α-tocopherol. However, the effect of these minor component concentration was inrrelevant since the concentration of these component was lower in the CPO for less than 1%. The solubility of HCW in CPO need to be monitored to avoid the hydrolysis which can deteriorate the CPO and degrade its quality.

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Structural Elucidation of the Masp1 and Masp2 protein from Nephila Pilipes Web via bioinformatics approaches

2023 , Anwardi Jamil , Amirul Ridzuan Abu Bakar , Nurul Husna Khairuddin

Major ampullate spidroin (MaSps) from orb-weaver spp spider has recently gained interest due to its exceptional characteristics. The biomechanical and biochemical properties from MaSps offer potential in harvesting and exploiting MaSps as a promising bio-based product. However, the current research on the structural elucidation focused more onto the Nephila clavipes spider web rather than the Nephila pilipes which are more common in this region. Herein, this study integrates the used of computational power and algorithm to elucidate the 3D protein morphology of MaSp1 and MaSp2 of Nephila pilipes dragline silk protein using nearly complete amino acid sequences obtained from the protein database (PDB). In silico homology modelling via Phyre2, SWISS-MODEL and RaptorX was adopted to predict the protein structure of MaSP-1 and 2 using proteins threading, automated comparative modelling of three-dimensional (3D) protein structures and deep learning approaches. Consequently, we described a thorough 3D protein models of MaSp1 and MaSp2 with a higher percentage of coils, α-helix and a low percentage of β-sheet on repetitive regions of MaSp1 and MaSp2. The results of this current work provide insights into Bioinformatics potentials in engineering spider silk-based biomaterial and bridging the most apparent gaps in the knowledge of MaSp1 and MaSp2.

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Insights into the Quality Control and Safety Assessment of Ficus Detoidea Varieties

2023-06-09 , Jamil A. , Khairuddin N.H. , Mohd Qalani Che Kasim , Mohd Arsad Johanis , Amirul Ridzuan Abu Bakar

An important aspect in herbal studies involved the understanding and evaluation on the quality and safety assessment of the raw materials before proceeding to herbal drug development process. In herbal standardization, the quality and safety assessment of herbal drugs offer an insight on the condition and handling of the raw material during the pre- and post-harvesting process. Thenceforth, a study was conducted to evaluate and construe the quality and safety assessment of four different varieties of Ficus deltoidea which were the Ficus deltoidea var. kunstleri, var. delotidea, var. terengganuensis and var. angustifolia. A series of quality control test and safety assessment were conducted on the leaf of Ficus deltoidea consist of total ash, moisture content, aflatoxins, total microbial content and heavy metal analysis. Overall, the analysis elucidated acceptable quality control results, with no aflatoxins and food pathogens were detected from all the samples, providing an insight on how the raw materials were handled, priorly. To summarized, the findings obtained from these studies showed that all the Ficus deltoidea varieties complied to proper pre- and post-harvesting handling process and are suitable for further herbal drug development study.

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Pharmacognostic Evaluation of Zingiber Officinale and Curcuma Longa from Perlis for Therapeutic Discovery

2023-01-01 , Jamil A. , San L.P. , Nik Muhammad Azhar Nik Daud , Mohd Asraf Mohd Zainudin , Mohd Qalani Che Kasim , Muhammad Izzat Ridzuan , Khairuddin N.H. , Amirul Ridzuan Abu Bakar

The medicinal properties of a plant are sought after for its potential therapeutic properties. Through its phytochemicals, numerous biological activities have been reported scientifically and were developed as an active pharmaceutical ingredient for biological products. Hence, this study evaluated the locally produced Zingiber officinale and Curcuma longa obtained from Padang Besar, Perlis, for their pharmacognostic properties. Quality control assessments were conducted to determine the cleanliness of the raw materials before proceeding with the pharmacognostic evaluation. The extracts for both plants were prepared through ultrasonic-assisted extraction (UAE) and maceration using methanol, ethanol, and distilled water. The phenolic compounds, flavonoids, and protein for each extract were investigated. The total phenolic compounds revealed that the combination of UAE and methanol had the highest value for both plant samples. On the other hand, the total flavonoid content suggests that UAE in ethanol solvent was the best approach to extract flavonoids for both plants. On the contrary, maceration techniques extracted the highest protein content for both plants. The biological testing for therapeutic evaluation based on the 2,2-diphenyl-1-picrylhydrazyl scavenging assay unraveled the methanol UAE was the potent extract for Z. officinale and C. longa, with half maximal inhibitory concentration, IC50 values of 0.39 and 0.32 mg/mL, respectively. Altogether, the findings obtained from these studies showed that Z. officinale and C. longa from Perlis showed good quality conditions, high content of phytochemicals, and potent antioxidant properties suitable for further herbal drug development studies.

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Optimization of Rice Bran Protein Extraction Using Choline Chloride-Glycerol Deep Eutectic Solvent Using Response Surface Methodology (RSM)

2023-01-01 , Kamal Ramlee K.A.F. , Muhammad Nor I.N. , Mohd Sharizan Md Sarip , Mohd Zainudin M.A. , Amirul Ridzuan Abu Bakar , Nawawi M.A. , Nik Muhammad Azhar Nik Daud

A by-product of the rice milling process, rice bran contains four different types of protein: albumin, globulin, prolamin, and glutelin. These proteins are widely known for being hypoallergenic and having nutritional benefits. In order to increase the value of rice bran, the protein was extracted via deep eutectic solvent (DES). Response Surface Methodology (RSM) was used to assess the impact of three operational conditions, including temperature, extraction duration, and the ratio of rice bran to DES, on the yield of rice bran protein after the precipitates were converted into powder form by freeze drying. At optimal working conditions, which were 60 Â°C, 2 h, and a 1:9 ratio of rice bran to DES, the method’s results showed that the highest extracted protein of rice bran was 16.254%. Several techniques, including Fourier transform infrared spectroscopy (FTIR) and Kjeldahl studies, have been used to demonstrate the presence of protein in rice bran powder. In the rice bran protein (RBP), amine (1640.70 cm−1), alcohol (3229.74 cm−1), and alkane (2925.37 cm−1) were all detected using FTIR analysis. Furthermore, Kjeldahl analysis revealed that 15.61% of the rice bran powder’s protein content is present. In conclusion, rice bran’s value as a functional meal can be increased by adding protein through the use of a deep eutectic solvent called green solvent.

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A Three-Level Factorial Model for Maximising Protein Extraction from Rice Bran with Choline Chloride: Glycerol

2024-05-01 , Ramlee K.A.F.K. , Daud N.M.A.N. , Mohd Sharizan Md Sarip , Amirul Ridzuan Abu Bakar , Zainudin M.A.M.

Rice bran, a by-product of rice milling, contains four protein types – albumin, globulin, prolamin, and glutelin – known for their hypoallergenic nature and nutritional advantages. To enhance rice bran's worth, its protein was isolated using deep eutectic solvent (DES). The influence of three operational factors (temperature, extraction time, and rice bran to DES ratio) on protein yield was evaluated post-conversion using three level factorial design. Optimal conditions at 80°C, 3 hours, and 1:5 rice bran to DES ratio yielded the highest extracted rice bran protein (RBP) at 17.49%. Temperature and solvent-to-sample ratio show the lowest p-value, indicating a significant effect on the RBP yield. The validation test shows an error of less than 5% between the experimental and the predicted value, showing the model can be used to predict the RBP yield. The RBP was then characterised using Fourier Transform Infrared Spectroscopy (FTIR), Bradford Assays and SDS-PAGE electrophoresis to confirm the protein presence in rice bran powder. FTIR analysis showed the existence of amide I, amide II, aliphatic groups and amine or hydroxyl groups in the RBP. Furthermore, Bradford assays analysis indicated 23.89 ± 0.75% protein content in rice bran powder. SDS-PAGE electrophoresis shows the existence of Albumin and globulin in the RBP. In conclusion, a full three level factorial can be used to model and predict the optimal condition for protein extraction from rice bran using ChCl-Gly DES.

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