An important aspect in herbal studies involved the understanding and evaluation on the quality and safety assessment of the raw materials before proceeding to herbal drug development process. In herbal standardization, the quality and safety assessment of herbal drugs offer an insight on the condition and handling of the raw material during the pre- and post-harvesting process. Thenceforth, a study was conducted to evaluate and construe the quality and safety assessment of four different varieties of Ficus deltoidea which were the Ficus deltoidea var. kunstleri, var. delotidea, var. terengganuensis and var. angustifolia. A series of quality control test and safety assessment were conducted on the leaf of Ficus deltoidea consist of total ash, moisture content, aflatoxins, total microbial content and heavy metal analysis. Overall, the analysis elucidated acceptable quality control results, with no aflatoxins and food pathogens were detected from all the samples, providing an insight on how the raw materials were handled, priorly. To summarized, the findings obtained from these studies showed that all the Ficus deltoidea varieties complied to proper pre- and post-harvesting handling process and are suitable for further herbal drug development study.