Nuradibah Mohd Amer
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Nuradibah Mohd Amer
Official Name
Nuradibah, Mohd Amer
Alternative Name
Mohd Amer, Nuradibah A.
Nuradibah, M. A.
Amer, Nuradibah Mohd M.
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Scopus Author ID
57219024135

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  • Publication
    Bioprotein optimization from spent mushroom substrate for fish feed application
    (AIP Publishing, 2020)
    Nuradibah Mohd Amer
    ;
    Z. Masyitah
    ;
    Sam Sung Ting
    ;
    Khairul Farihan Kasim
    ;
    Nik Noriman Zulkepli
    ;
    Omar S. Dahham
    ;
    Saad S. Dahham
    The utilization of spent mushroom substrate as potential substrate for bioprotein production is being explored in this study. The objectives of this study were to screen three different types of fungi in bioprotein production from spent mushroom substrate and to optimize the process condition of the best fungi for production of bioprotein by using Design Expert Software. In this present study, screening of three different strains; Aspergillus terreus UniMAP AA-1, Aspergillus niger (ATCC 16404) and Phanerochaete chrysosporium (ATCC 24725), was done for bioprotein production by solid state fermentation process. P. chrysosporium produced the highest amount of protein on the sixth day, with the amount of 0.3951 mg/mL. Optimization of two parameters; substrate concentration and inoculum size for the selected fungi, P. chrysosporium was done conducted by Response Surface Methodology (RSM): Central Composite Design (CCD) to investigate optimal condition of bioprotein production. The optimum condition for P. chrysosporium to produce bioprotein using SMS was achieved at 60% of substrate concentration and 15% (w/v) of inoculum size with maximum protein concentration of 0.3812 mg/mL.
  • Publication
    Kinetic Extraction of Antioxidant and Total Phenoloc Content of Clinachantus nutans
    ( 2020-07-09)
    Aqilah Abd Rahim, Syafiqah
    ;
    Nuradibah Mohd Amer
    ;
    Khairul Farihan Kasim
    Clinacanthus nutans or Belalai Gajah is one of the herbs that contain natural antioxidant. This natural antioxidant can be used commercially in food as well as pharmaceutical industries. This research explored antioxidant of C. nutans and it was extracted by using solid-liquid extraction. Antioxidant of C. nutans extract was determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and Total Phenolic Content (TPC) were determined using Folic-Ciocalteau reagent. Screening of the suitable particle size to be used in the extraction of C. nutans shows that mixture and leaves of C. nutans with particle size <63 μm was the best to obtain the highest DPPH scavenging activity. However, only mixture of C. nutans sample was used for the thermal extraction kinetic due to the availability of the samples. Thermal extraction kinetic of DPPH was fitted to the exponential growth model and show a good fit with R^2 = 0.9921. When transformed, the data gave a linear Arrhenius plot, R^2=0.717 with an activation energy of 17.35 x 0.108 kJ/mol. Thermal extraction kinetic of TPC was fitted to the exponential growth model and show a good fit with R^2 = 0.9892. When transformed, the data gave a linear Arrhenius plot, R^2=0.897 with an activation energy of 5.3832 x 0.451 kJ/mol. The model is adequate to predict DPPH scavenging activity and TPC of the C. nutans extracts with no significant different of the prediction and validation conducted at temperature of 70 °C for 30 min.
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