Noor Hasyierah Mohd Salleh
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Noor Hasyierah Mohd Salleh
Official Name
Noor Hasyierah, Mohd Salleh
Alternative Name
Salleh, N. H.M.
Salleh, N. H.Mohd
Mohd Salleh, N. H.
Mohd Salleh, Noor Hasyierah
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Scopus Author ID
41762655600

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  • Publication
    Valorization of Momordica charantia seeds into phytogenically synthesized silver nanoparticles for the protection of oyster mushrooms against Trichoderma pleuroticola
    ( 2023-12-01)
    Norhidayah Abd Aziz
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Subash Chandra Bose Gopinath
    ;
    Kamaruddin A.H.
    ;
    Muhammad Izzat Ridzuan
    ;
    Ahmad N.A.
    Green mold caused by Trichoderma pleuroticola is the most invasive disease of oyster mushrooms, causing massive losses worldwide. Herein, we valorized food waste, the Momordica seed extract (MSE), into silver nanoparticles (AgNPs-MSE). The synthesis parameters were optimized, and the biogenic AgNPs showed a remarkable antifungal activity against T. pleuroticola. Quantitative and qualitative analyses showed that the MSE was rich in bioactive compounds. The highly stable AgNPs-MSE was synthesized under the following conditions: MSE–AgNO3 ratio, 5:95; synthesis time, 9 min; temperature, 60 °C; and pH, 8.0. The UV absorption spectra showed surface plasma resonance peak at 428 nm, confirming the presence of spherical nanoparticles. FTIR spectra ascertained the involvement of phytoconstituents in the MSE during synthesis, and X-ray diffraction analysis revealed a face-centered cubic (fcc) crystalline structure with an average particle size of 11.34 nm. HR-TEM analysis corroborated the spherical shape, with a nanoparticle size of 8.0 nm. EDX analysis revealed the presence of silver, carbon, oxygen, nitrogen, sulfur, and chlorine in the AgNPs-MSE, which was further substantiated by XPS analysis. A zeta potential of − 29.7 mV and PDI of 0.144 revealed the good stability and uniformity of the synthesized AgNPs-MSE. The optimized AgNPs-MSE exhibited a potent antifungal activity against T. pleuroticola at 300 ppm in vitro. SEM and TEM analyses of AgNPs-MSE-treated T. pleuroticola showed hyphal system breakage and leakage of cytoplasmic contents that leads to cell death. Therefore, the use of the AgNPs-MSE as a nanoparticle-based fungicide has the potential to effectively control T. pleuroticola for oyster mushroom protection.
      2
  • Publication
    Botanical insecticide of chili and ginger extract on Nilaparvata lugens, brown planthopper
    (IOP Publishing Ltd., 2020)
    Noor Hasyierah Mohd Salleh
    ;
    Norhidayah Abd Aziz
    ;
    Alina Rahayu Mohamed
    ;
    Adilah Anuar
    ;
    I Nur Humaira
    Invasion of Nilaparvata lugens or brown planthopper (BPH) in paddy fields has led to the use of excessive chemical insecticides due to its effectiveness. However, excessive applications has contributed several negative effects on the environment, farmers as well as non-targeting organisms. As an alternative, a mixture of a botanical insecticide containing mixture of chili and ginger extracts have been studied. Three parameters were studied namely the extract concentration, time of exposure and temperature of exposure. The results showed that 90% of BPH mortality was found at 40% extract concentration with 72 hr duration time of exposure at optimal temperature 30 C. HPLC analysis proved the presence of capsaicin and gingerol at peaks of 4.502 min and 11.046 min respectively. From repellency analysis, the BPH showed selective repel action against the treated paddy. This is due to the pungent odor contributed from compound of gingerol, shogaol and capsaicin presented in the mixture. Meanwhile, contact toxicity studies have successfully give 100% of mortality of BPH. Microscopic analysis have shown BPH deformities was increased by the extract concentration.
      3  3
  • Publication
    Growth Optimisation of Bacillus subtilis in medium supplemented with prebiotic gum Arabic
    ( 2020-12-18)
    Norhidayah Abd Aziz
    ;
    Noor Hasyierah Mohd Salleh
    ;
    Adilah Anuar
    ;
    Amira Farzana Samat
    ;
    Lian Ou Li
    ;
    Siti Aminah Mohd Hassan
    ;
    Abu Hassan A.F.
    Bacillus subtilis is a type of gastrointestinal bacteria that build up a healthy gut microbiota. The bacterial species has been well documented to involve in maintaining a healthy gut homeostasis in which disruption in it's microbial composition or "dysbiosis"has recently been linked to obesity. Probiotic in vivo restoration of dysbiosed microbiota have been proved successful in obese CD-1 mice model. However, prebiotic study using Gum Arabic (GA) assessing to optimise an in vitro lumen system specifically targeting to enhance the growth of B. subtilis are still lacking since this bacteria are depleted in obese individuals. Thus, this study aimed to establish the optimal growth conditions in simulated in vitro lumen system and to the best of our knowledge, this is the first in vitro study attempted to optimize the growth of B. subtilis in medium supplemented with prebiotic GA. Growth screening analysis suggested an optimal dosage of 1.0% and 0.5% glucose and GA, respectively. The highest growth rate was recorded at 0.7995 hours-1 with doubling time of 52.02 minutes with extended period of stationary phase. The optimal GA concentration and fermentation conditions were determined at 0.67%, pH 7.4 and temperature of 37 C. The validated suggested model indicates that the supplementation of GA into an optimal fermentation systems is promising to enhance the growth of gut microflora Bacillus subtilis, for restoration of a dysbiosed gut microbiota in vitro.
      1  18