Segmentation of blood vessels in the retinal is a crucial step in the diagnosis of eye diseases such as diabetic retinopathy and glaucoma. This paper presents a supervised method for automatic segmentation of blood vessels in retinal images. The proposed method based on a hybrid combination between Gray-Level and Moment Invariant techniques. There are four steps involved, whereas preprocessing, feature extraction, classification, and post-processing. In the preprocessing, three stages are performed include vessel central light reflex removal, background homogenization, and vessel enhancement. The 7-D vector feature extraction was performed to compute that compose of gray-level and moment invariants-based features for pixel representation. The decision tree is used for classification step that characterized the pixel based on vessels and non-vessels. The final step is the post-processing which will remove the small artifacts appears after classification process. The proposed method was compared to the Vascular Tree method and Morphological method. Based on the objective evaluation, the proposed method achieved (sensitivity = 98.589, specificity =55.544 and accuracy = 96.197).
