This paper describes for the first time the synthesis of hydroxyapatite based on bio-ceramic hollow fiber membrane originating from Tilapia fish bones (bio-CHFM/HApT). Prior to the fabrication process, Tilapia fish bones were converted into hydroxyapatite powder by simple calcination method. A well-known method of membrane fabrication; a combination of phase inversion and sintering technique has been used. The study revealed that an exceptional asymmetric structure comprised of desired finger-like and sponge-like structure was induced by the bio-CHFM/HApT. Interestingly, bio-CHFM/HApT sintered at 1100 °C can induced hybrid adsorption/separation for As(III) removal at separation efficiency up to 99.9% and high flux of ∼250 kg/m2h. Additionally, the equilibrium adsorption capacities were obtained within 40 min of contact time. The kinetic information of the removal of As(III) bio-CHFM/HApT is the most suitable for pseudo-first order.