Surface acoustic wave (SAW) devices are widely used for cell manipulation and cell separation in cellular biology research. Despite the fact that the SAW stimulation is biocompatible and noninvasive, it is still unknown how cells will respond to the stimulation in terms of migration. In this study, we developed two types of SAW device model with different frequency at 83 MHz and 124 MHz and investigated the effect of standing SAW stimulation to the cells model. We also fabricated the SAW devices to measure its electrical properties. As a result, different local stresses along cell-fluid boundaries was observed as different frequencies of standing SAW were applied to the cells. The standing SAW with higher frequency ascribed to a higher stress at the cell's apical and basal as compared to lower frequency stimulation. Moreover, the fabricated SAW shows a low insertion loss at −19.8 dB and −13.5 dB for the 83 MHz and 124 MHz of SAW, respectively. Based on these results, the standing SAW can be used as an alternative tool in regulating the aberrant cellular activities related with cell migration.
