Publication:
High-performance interactive analysis of split aptamer and HIV-1 Tat on multiwall carbon nanotube-modified field-effect transistor

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cris.author.scopus-author-id 56395436500
cris.author.scopus-author-id 57194735807
cris.author.scopus-author-id 7006558013
cris.author.scopus-author-id 57211870224
dc.contributor.author Fatin M.F.
dc.contributor.author Rahim Ruslinda A.
dc.contributor.author Gopinath S.C.B.
dc.contributor.author Arshad M.K.M.
dc.date.accessioned 2024-12-14T07:37:13Z
dc.date.available 2024-12-14T07:37:13Z
dc.date.issued 2019-03-15
dc.description.abstract Interaction between split RNA aptamer and the clinically important target, HIV-1 Tat was investigated on a biosensing surface transduced by functionally choreographed multiwall carbon nanotubes (MWCNTs). Acid oxidation was performed to functionalize MWCNTs with carboxyl functional groups. X-ray photoelectron spectroscopy analysis had profound ~2.91% increment in overall oxygen group and ~1% increment was noticed with a specific carboxyl content owing to C[dbnd]O and O–C[dbnd]O bonding. The interaction between split RNA aptamer and HIV-1 Tat protein was quantified by electrical measurements with the current signal (Ids) over a gate voltage (Vgs). Initially, 34.4 mV gate voltage shift was observed by the immobilization of aptamer on MWCNT. With aptamer and HIV-1 Tat interaction, the current flow was decreased with the concomitant gate voltage shift of 23.5 mV. The attainment of sensitivity with split aptamer and HIV-1 Tat interaction on the fabricated device was 600 pM. To ensure the genuine interaction of aptamer with HIV-1 Tat, other HIV-1 proteins, Nef and p24 were interacted with aptamer and they displayed the negligible interferences with gate voltage shift of 3.5 mV and 5.7 mV, which shows 4 and 2.5 folds lesser than HIV-1 Tat interaction, respectively.
dc.identifier.doi 10.1016/j.ijbiomac.2018.12.066
dc.identifier.pmid 30529550
dc.identifier.scopus 2-s2.0-85058211099
dc.identifier.uri https://hdl.handle.net/20.500.14170/11011
dc.relation.funding Universiti Malaysia Perlis
dc.relation.grantno undefined
dc.relation.ispartof International Journal of Biological Macromolecules
dc.relation.ispartofseries International Journal of Biological Macromolecules
dc.relation.issn 01418130
dc.subject Field effect transistor | HIV-1 Tat | Multiwall carbon nanotube | Split aptamer
dc.title High-performance interactive analysis of split aptamer and HIV-1 Tat on multiwall carbon nanotube-modified field-effect transistor
dc.type Journal
dspace.entity.type Publication
oaire.citation.endPage 422
oaire.citation.startPage 414
oaire.citation.volume 125
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
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person.identifier.scopus-author-id 56395436500
person.identifier.scopus-author-id 57194735807
person.identifier.scopus-author-id 7006558013
person.identifier.scopus-author-id 57211870224
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