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  1. Home
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  3. Institute of Nano Electronic Engineering (INEE)
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  5. A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase
 
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A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase

Journal
Sensors
ISSN
1424-8220
Date Issued
2012
Author(s)
Syed M. Usman Ali
Linköping University, Sweden
Zafar Hussain Ibupoto
Linköping University, Sweden
Muhammad Kashif
Universiti Malaysia Perlis
Uda Hashim
Universiti Malaysia Perlis
Magnus Willander
Linköping University, Sweden
DOI
10.3390/s120302787
Abstract
In the present work zinc oxide nanoflakes (ZnO-NF) structures with a wall thickness around 50 to 100 nm were synthesized on a gold coated glass substrate using a low temperature hydrothermal method. The enzyme uricase was electrostatically immobilized in conjunction with Nafion membrane on the surface of well oriented ZnO-NFs, resulting in a sensitive, selective, stable and reproducible uric acid sensor. The electrochemical response of the ZnO-NF-based sensor vs. a Ag/AgCl reference electrode was found to be linear over a relatively wide logarithmic concentration range (500 nM to 1.5 mM). In addition, the ZnO-NF structures demonstrate vast surface area that allow high enzyme loading which results provided a higher sensitivity. The proposed ZnO-NF array-based sensor exhibited a high sensitivity of ~66 mV/ decade in test electrolyte solutions of uric acid, with fast response time. The sensor response was unaffected by normal concentrations of common interferents such as ascorbic acid, glucose, and urea
Subjects
  • ZnO nanoflakes (ZnO-N...

  • Potentiometric nanose...

  • Uricase

  • Nafion ® membrane

File(s)
A Potentiometric Indirect Uric Acid Sensor Based on ZnO Nanoflakes and Immobilized Uricase.pdf (1.3 MB)
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