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A needle-like Cu<inf>2</inf>CdSnS<inf>4</inf> alloy nanostructure-based integrated electrochemical biosensor for detecting the DNA of Dengue serotype 2
A needle-like Cu<inf>2</inf>CdSnS<inf>4</inf> alloy nanostructure-based integrated electrochemical biosensor for detecting the DNA of Dengue serotype 2
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Date
2017-07-01
Authors
Odeh A.A.
Al-Douri Y.
Voon C.H.
Mat Ayub R.
Gopinath S.C.B.
Odeh R.A.
Ameri M.
Bouhemadou A.
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Abstract
The authors describe an integrated biosensor for amperometric DNA detection of Dengue virus in real time. Cu2CdSnS4 (CCTS) quaternary alloy nanostructures were successfully synthesized, deposited on an oxygen-etched silicon substrate (O2/Si) via spin coating, and annealed at 400 °C. The nanostructures were investigated by using UV-vis spectroscopy, X-ray diffraction, atomic force microscopy and scanning electron microscopy. Interdigitated electrodes were fabricated using silver as a metal contact deposited on the CCTS/O2/Si substrate using a thermal evaporator vacuum coater and a hard mask. The quaternary alloy acts as a support for immobilization of a Dengue-specific DNA probe that is employed as the recognition element. Single-stranded DNA in concentrations from 100 f. to 10 nM were successfully recognized via amperometry, typically at a working voltage of 1.5 V. The lower detection limit is 17 nM. Sensitivity is found to be 24.2 μA nM−1 cm−2. The biosensor is inexpensive, fast, highly sensitive, and has low power consumption.
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Keywords
Band gap | Capacitance | Concentrations | DNA probe | Electrical properties | Immobilization | Impedance | Interdigitated electrodes | Nanostructures | Sensitivity