Incorrect running may lead to discomfort and injuries, where each day around the world, the numbers of runners are increasing. The goal of this research work is to evaluate and study the lower limb muscles fatigue during running for 400-meters with two types of running strategies based on the Electromyography (EMG) signals. The EMG signals are collected from Rectus Femoris (RF), Biceps Femoris (BF), and Gastrocnemius Lateralis (GL) muscles during the run on the tartan athletic track with two types of running strategies. The first type: the first 200-meters running with normal speed and the last 200-meters running with full speed. The second type: the first 300-meters running with normal speed and the last 100-meters running with full speed. The EMG signals were transformed into the frequency domain using fast Fourier transform (FFT) to extract the features of mean frequency (MNF) and median frequency (MDF). From the results of the two strategies with MDF and MNF features of the selected muscles, the lowest fatigue index was during the 1st strategy for most the selected muscles.
