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  4. Publications 2017
  5. A direct detection of human papillomavirus 16 genomic DNA using gold nanoprobes
 
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A direct detection of human papillomavirus 16 genomic DNA using gold nanoprobes

Journal
International Journal of Biological Macromolecules
ISSN
01418130
Date Issued
2017-01-01
Author(s)
Azizah N.
Hashim U.
Gopinath S.C.B.
Nadzirah S.
DOI
10.1016/j.ijbiomac.2016.10.060
Handle (URI)
https://hdl.handle.net/20.500.14170/11655
Abstract
Nanoparticles have been investigated as flagging tests for the sensitive DNA recognition that can be utilized as a part of field applications to defeat restrictions. Gold nanoparticles (AuNPs) have been widely utilized due to its optical property and capacity to get functionalized with a mixed bag of biomolecules. This study exhibits the utilization of AuNPs functionalized with single-stranded oligonucleotide (AuNP-oligo test) for fast the identification of Human Papillomavirus (HPV). This test is displayed on interdigitated electrode sensor and supported by colorimetric assay. DNA conjugated AuNP has optical property that can be controlled for the applications in diagnostics. With its identification abilities, this methodology incorporates minimal effort, strong reagents and basic identification of HPV.
Funding(s)
Ministry of Higher Education, Malaysia
Subjects
  • Colorimetric detectio...

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