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Voltammetric DNA Biosensor for Human Papillomavirus (HPV) Strain 18 Detection

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cris.virtual.department Universiti Malaysia Perlis
cris.virtual.department Universiti Malaysia Perlis
cris.virtual.department Universiti Malaysia Perlis
cris.virtual.department Universiti Malaysia Perlis
cris.virtual.department Universiti Malaysia Perlis
cris.virtualsource.department 9fb002ae-804a-408b-a407-12683fa3a7bd
cris.virtualsource.department cbc99d4d-6211-466b-bb62-06960f8c24ca
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cris.virtualsource.department f61ba939-dfbd-4d1f-9275-752ad9b8e59d
dc.contributor.author Mhd Akhir M.A.
dc.contributor.author Nor Azizah Parmin
dc.contributor.author Uda Hashim
dc.contributor.author Subash Chandra Bose Gopinath
dc.contributor.author Rejali Z.
dc.contributor.author Afzan A.
dc.contributor.author Muhammad Nur Aiman Uda
dc.contributor.author Muhammad Nur Afnan Uda
dc.contributor.author Voon Chun Hong
dc.date.accessioned 2024-09-28T04:36:07Z
dc.date.available 2024-09-28T04:36:07Z
dc.date.issued 2020-07-09
dc.description.abstract This research was developed to focus on the study of the voltammetric DNA biosensor for the detection of HPV strain 18. In this research, electrical DNA biosensor was expected to detect HPV strain 18 more efficiently by using electrical characterization. In this project, device inspection was conducted to make sure the functional of the gold interdigitated electrode (IDE) by using Scanning Electron Microscope (SEM). 3-Aminopropyl Triethoxysilane (APTES) solution was used for the process of surface modification to form the amine group on the surface of the device to facilitate the attachment of the DNA probe. In this project, synthetic DNA sample and DNA from the saliva of several Biosystems Engineering students were used as the target DNA. The current-voltage (I-V) electrical characterization was conducted to detect the presence of HPV strain 18 in both DNA samples. As the results, perfect alignment between the electrodes on the IDE was detected under SEM. Surface modification of the biosensor successfully conducted which is the covalent bond between APTES and DNA probe increase the electrical. Synthetic DNA shows the presence of HPV strain 18 while there was no HPV strain 18 detected in the DNA from saliva samples.
dc.identifier.doi 10.1088/1757-899X/864/1/012166
dc.identifier.scopus 2-s2.0-85091062992
dc.identifier.uri https://hdl.handle.net/20.500.14170/5288
dc.language.iso en
dc.relation.funding Ministry of Higher Education
dc.relation.grantno undefined
dc.relation.ispartof IOP Conference Series: Materials Science and Engineering
dc.relation.ispartofseries IOP Conference Series: Materials Science and Engineering
dc.relation.issn 17578981
dc.rights open access
dc.title Voltammetric DNA Biosensor for Human Papillomavirus (HPV) Strain 18 Detection
dc.type Conference Proceeding
dspace.entity.type Publication
oaire.citation.issue 1
oaire.citation.volume 864
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Putra Malaysia
oairecerif.affiliation.orgunit Universiti Putra Malaysia
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
oairecerif.affiliation.orgunit Universiti Malaysia Perlis
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oairecerif.author.affiliation Universiti Malaysia Perlis
oairecerif.author.affiliation Universiti Malaysia Perlis
oairecerif.author.affiliation Universiti Malaysia Perlis
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oairecerif.author.affiliation Universiti Malaysia Perlis
oairecerif.author.affiliation Universiti Malaysia Sabah
oairecerif.author.affiliation Universiti Malaysia Perlis
oairecerif.citation.number 012166
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person.identifier.scopus-author-id 57219023141
person.identifier.scopus-author-id 57195835481
person.identifier.scopus-author-id 22633937800
person.identifier.scopus-author-id 7006558013
person.identifier.scopus-author-id 56545685600
person.identifier.scopus-author-id 58313807500
person.identifier.scopus-author-id 55984680000
person.identifier.scopus-author-id 57195307027
person.identifier.scopus-author-id 55334719400
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