Sensitive, selective, and stable materials are important to act as an electron transport layer to construct signals in biosensors. Graphene-based biosensors get attention in this industry due to their selective and sensitive properties. We fabricated TiO₂/rGO and TiO₂ using spin coating in this study. Thermal evaporation deposited the silver. The picoammeter examined the influence of microelectrode gap sizes (50, 100, and 250 μm) on electrical performance. It was revealed that a smaller gap generates better electrical performance. At the smallest gap, the 50μm TiO2 (8.04E-12 A) and TiO₂/rGO (2.385E-8 A) at 0.04V. The morphological and chemical analysis supported the electrical behavior of TiO₂/rGO and TiO₂. These results show that the method used was able to form a TiO₂/rGO film that is sensitive to the size of the gap between two microelectrodes.
