Publication:
Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures
Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures
| cris.author.scopus-author-id | 57196607196 | |
| cris.author.scopus-author-id | 22633937800 | |
| cris.author.scopus-author-id | 7006558013 | |
| cris.author.scopus-author-id | 57194266103 | |
| cris.author.scopus-author-id | 37012449500 | |
| cris.author.scopus-author-id | 57198446841 | |
| cris.author.scopus-author-id | 26435546400 | |
| cris.author.scopus-author-id | 55334719400 | |
| cris.author.scopus-author-id | 7005958999 | |
| dc.contributor.author | Ten S. | |
| dc.contributor.author | Hashim U. | |
| dc.contributor.author | Gopinath S. | |
| dc.contributor.author | Liu W. | |
| dc.contributor.author | Foo K. | |
| dc.contributor.author | Sam S. | |
| dc.contributor.author | Rahman S. | |
| dc.contributor.author | Voon C. | |
| dc.contributor.author | Nordin A. | |
| dc.date.accessioned | 2025-01-13T07:03:31Z | |
| dc.date.available | 2025-01-13T07:03:31Z | |
| dc.date.issued | 2017-07-15 | |
| dc.description.abstract | Surface acoustic wave mediated transductions have been widely used in the sensors and actuators applications. In this study, a shear horizontal surface acoustic wave (SHSAW) was used for the detection of food pathogenic Escherichia coli O157:H7 (E.coli O157:H7), a dangerous strain among 225 E. coli unique serotypes. A few cells of this bacterium are able to cause young children to be most vulnerable to serious complications. Presence of higher than 1 cfu E.coli O157:H7 in 25 g of food has been considered as a dangerous level. The SHSAW biosensor was fabricated on 64° YX LiNbO3 substrate. Its sensitivity was enhanced by depositing 130.5 nm thin layer of SiO2 nanostructures with particle size lesser than 70 nm. The nanostructures act both as a waveguide as well as a physical surface modification of the sensor prior to biomolecular immobilization. A specific DNA sequence from E. coli O157:H7 having 22 mers as an amine-terminated probe ssDNA was immobilized on the thin film sensing area through chemical functionalization [(CHO-(CH2)3-CHO) and APTES; NH2-(CH2)3-Si(OC2H5)3]. The high-performance of sensor was shown with the specific oligonucleotide target and attained the sensitivity of 0.6439 nM/0.1 kHz and detection limit was down to 1.8 femto-molar (1.8×10−15 M). Further evidence was provided by specificity analysis using single mismatched and complementary oligonucleotide sequences. | |
| dc.identifier.doi | 10.1016/j.bios.2016.09.035 | |
| dc.identifier.pmid | 27660016 | |
| dc.identifier.scopus | 2-s2.0-84994718062 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14170/11580 | |
| dc.relation.funding | Kementerian Sains, Teknologi dan Inovasi | |
| dc.relation.grantno | RM-5022NF10 | |
| dc.relation.ispartof | Biosensors and Bioelectronics | |
| dc.relation.ispartofseries | Biosensors and Bioelectronics | |
| dc.relation.issn | 09565663 | |
| dc.subject | Deoxyribonucleic acid (DNA) | E. coli O157:H7 | Hybridization | Shear horizontal surface acoustic wave (SHSAW) | Surface functionalization | |
| dc.title | Highly sensitive Escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures | |
| dc.type | Journal | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 154 | |
| oaire.citation.startPage | 146 | |
| oaire.citation.volume | 93 | |
| oairecerif.affiliation.orgunit | Institut Penyelidikan dan Kemajuan Pertanian Malaysia | |
| oairecerif.affiliation.orgunit | Universiti Malaysia Perlis | |
| oairecerif.affiliation.orgunit | Universiti Malaysia Perlis | |
| oairecerif.affiliation.orgunit | Universiti Malaysia Perlis | |
| oairecerif.affiliation.orgunit | Universiti Malaysia Perlis | |
| oairecerif.affiliation.orgunit | Universiti Malaysia Perlis | |
| oairecerif.affiliation.orgunit | Universiti Putra Malaysia | |
| oairecerif.affiliation.orgunit | Universiti Malaysia Perlis | |
| oairecerif.affiliation.orgunit | International Islamic University Malaysia | |
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| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | |
| person.identifier.scopus-author-id | 57196607196 | |
| person.identifier.scopus-author-id | 22633937800 | |
| person.identifier.scopus-author-id | 7006558013 | |
| person.identifier.scopus-author-id | 57194266103 | |
| person.identifier.scopus-author-id | 37012449500 | |
| person.identifier.scopus-author-id | 57198446841 | |
| person.identifier.scopus-author-id | 26435546400 | |
| person.identifier.scopus-author-id | 55334719400 | |
| person.identifier.scopus-author-id | 7005958999 |