Spent mushroom substrate (SMS) is the biomass waste produced from the production of mushroom which generating disposal problems. To overcome the problem the extraction of bioactive compound such as β-glucan from the waste SMS could solve the problem and can also increase its added value. β-glucan appears to be promising for aiding in the cure of tumorous disease and help to reduce the cholesterol levels in blood. In this study, β-glucan was extracted and compared from two different commercial mushroom (Pleurotus ostreatus and Schizophyllum commune) from its fruiting body and mycelium on solid waste SMS using chemical extraction methods. The characteristics of physical structure, functional group and properties of extracted β-glucan was investigated. Here, Fourier-transform infrared (FTIR) and screening electron microscope (SEM) were used to identify and evaluated the structural conformations of β-glucan and physical structure. The functional properties, swelling power, viscosity and fat binding capacity were analyzed. Based on results, mycelia of Pleurotus ostreatus shown highest swelling power (11.74 g/g) and fat binding capacity (12.09 g oil/g sample) while, mycelia of Schizophyllum commune shown the highest viscosity (11.85 cP). Since the value for all functional properties shown the highest value on mycelium compare to fruiting body, thus it is strength that β-glucan extraction from mycelium solid waste has high novel properties compare with mushroom fruiting body.
