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Advancing COVID-19 detection high-performance RNA biosensing via electrical interactions

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cris.virtual.department Universiti Malaysia Perlis
cris.virtual.department Universiti Malaysia Perlis
cris.virtual.department Universiti Malaysia Perlis
cris.virtualsource.department cbc99d4d-6211-466b-bb62-06960f8c24ca
cris.virtualsource.department 8e981558-c63e-4656-a506-43e62c5327b0
cris.virtualsource.department 5fb9bf61-ee23-4db8-b8e2-faf2c2a04c7a
dc.contributor.author Uda Hashim
dc.contributor.author Muhammad Nur Afnan Uda
dc.contributor.author Muhammad Nur Aiman Uda
dc.contributor.author Tijjani Adam
dc.contributor.author Nur Hulwani Ibrahim
dc.contributor.author Chai Chang Yii
dc.contributor.author Lorita Angeline
dc.date.accessioned 2024-11-06T02:12:11Z
dc.date.available 2024-11-06T02:12:11Z
dc.date.issued 2024-06
dc.description.abstract This research paper investigated the detection of COVID-19 using an Aluminum Interdigitated Electrode (Al-IDE) sensor based on electrical conductivity. The silanization process involved the functionalization step, employing (3-Aminopropyl) triethoxysilane (APTES), while the immobilization process was facilitated by the RNA Probe specific to COVID-19. To verify its specificity in detection, the functionalized biosensor was tested against single-base mismatches, non-complementary sequences, and complementary sequences. The physical characteristics of the Al-IDE biosensor were examined using both low-power microscopy (LPM) and high-power microscopy (HPM). Additionally, the morphological properties of the biosensor were assessed using atomic force microscopy (AFM). To assess its diagnostic potential, the biosensor's sensitivity was evaluated by exposing it to a range of complementary targets, spanning from 1 femtomolar (fM) to 1 micromolar (μM). The current-voltage (I-V) characteristics of the biosensor were meticulously analyzed at each stage of functionalization bare Al-IDE, silanization, immobilization, and hybridization. This I-V characterization was carried out using a picoammeter voltage source (Keithley 2450), Kickstart software, and a probe station. The results confirmed the biosensor's capability to effectively detect COVID-19 targets within the nanoampere concentration range, demonstrating its success in detecting specific COVID-19 targets at the nanoampere level.
dc.identifier.doi 10.58915/ijneam.v17iJune.862
dc.identifier.uri https://ejournal-unimap-edu-my.ezproxyunimap.idm.oclc.org/index.php/ijneam/article/view/862/550
dc.identifier.uri https://ejournal-unimap-edu-my.ezproxyunimap.idm.oclc.org/index.php/ijneam/article/view/862
dc.language.iso en
dc.relation.ispartof International Journal of Nanoelectronics and Materials (IJNeaM)
dc.subject Aluminium Interdigitated Electrode
dc.subject Biosensor
dc.subject RNA COVID-19
dc.subject Selective
dc.subject Sensitive
dc.title Advancing COVID-19 detection high-performance RNA biosensing via electrical interactions
dc.type Resource Types::text::journal::journal article
dspace.entity.type Publication
oaire.citation.endPage 235
oaire.citation.issue 6
oaire.citation.startPage 229
oaire.citation.volume 17
oairecerif.author.affiliation Universiti Malaysia Perlis
oairecerif.author.affiliation Universiti Malaysia Sabah
oairecerif.author.affiliation Universiti Malaysia Perlis
oairecerif.author.affiliation Universiti Malaysia Perlis
oairecerif.author.affiliation Universiti Malaysia Perlis
oairecerif.author.affiliation Universiti Malaysia Sabah
oairecerif.author.affiliation Universiti Malaysia Sabah
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