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  5. Nanobioprobe for the determination of pork adulteration in burger formulations
 
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Nanobioprobe for the determination of pork adulteration in burger formulations

Journal
Journal of Nanomaterials
ISSN
1687-4110
1687-4129
Date Issued
2012-02-14
Author(s)
Mohamad Eaqub Ali
Universiti Malaysia Perlis
S. Mustafa
Universiti Putra Malaysia
Uda Hashim
Universiti Malaysia Perlis
Y. B. Che Man
Universiti Putra Malaysia
K. L. Foo
Universiti Malaysia Perlis
DOI
10.1155/2012/832387
Abstract
We report the development of a swine-specific hybrid nanobioprobe through a covalent integration of a fluorophore-labeled 27-nucleotide<jats:italic>Alu</jats:italic>I-fragment of swine cytochrome b gene to a 3 nm gold nanoparticle for the determination of pork adulteration in processed meat products. We tested the probe to estimate adulterated pork in ready-to-eat pork-spiked beef burgers. The probe quantitatively detected 1–100% spiked pork in burger formulations with ≥90% accuracy. A plot of observed fluorescence against the known concentration of<jats:italic>Alu</jats:italic>I-digested pork DNA targets generated a concave curve, demonstrating a power relationship (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>y</mml:mi><mml:mo>=</mml:mo><mml:mn>2.956</mml:mn><mml:msup><mml:mi>x</mml:mi><mml:mrow><mml:mn>0.509</mml:mn></mml:mrow></mml:msup></mml:math>) with a regression coefficient (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msup><mml:mi>R</mml:mi><mml:mn>2</mml:mn></mml:msup></mml:math>) of 0.986. No cross-species detection was found in a standard set of pork, beef, chicken, mutton, and chevon burgers. The method is suitable for the determination of very short-length nucleic acid targets which cannot be estimated by conventional and real-time PCR but are essential for the determination of microRNA in biodiagnostics and degraded DNA in forensic testing and food analysis.
Subjects
  • Genes

  • Polymerase chain reac...

  • Probes

  • Meats

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Nanobioprobe for the determination of pork adulteration in burger formulations.pdf (1.5 MB)
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