In the development of electrocardiography (ECG) systems for long term monitoring, flexible circuit is often required. However, polymer-based circuits usually required more complex fabrication process and possess limited flexibility. In this study, flexible electro-conductive pattern using cotton fabric as the substrate is proposed. The purpose of this study is to investigate the effect of mechanical disturbance to the cotton fabric-based conductive patterns. Reduced graphene oxide is used as the conductive material. The electro-conductive pattern is fabricated using wax patterning and pipetting methods. A 20 mm long and 1 mm wide electro-conductive pattern is fabricated and pipetted with 10 layers of conductive ink. The results show that 70 % of the electro-conductive pattern remained its conductance after 5 times of washing process. Besides, the conductive pattern remained higher than 60% of its conductance after 10 acute and obtuse folding cycles, respectively.The electroconductive line has been proven to possess a high stability against the mechanical disturbances which is one of the vital characteristics for a flexible circuit.