Publication:
Diagnosis of hepatitis B virus by an aptasensor developed on a microelectrode
Diagnosis of hepatitis B virus by an aptasensor developed on a microelectrode
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Date
2025-03
Authors
Jintang Yin
Yuefang Pan
Subash Chandra Bose Gopinath
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Publisher
Springer Science and Business Media Deutschland GmbH
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Abstract
Hepatitis B is a viral infection (HBV) that affects millions of people worldwide and causes HBV-related liver diseases, leading to both chronic and acute complications. Patients affected by HBV need a regular monitoring of disease progression and viral activity for a treatment and to reduce the risk. In this research, a highly sensitive HBsAg (hepatitis B surface antigen) aptasensor was developed on an interdigitated microelectrode (IDME). Amine-modified gold nanoparticles (Au NPs) were used as the linker to attach a capture probe of aptamer on IDME. To improve the aptamer immobilization, COOH-ended aptamer was premixed with glutaraldehyde and attached on IDME through the amine-Au NPs. This method of aptamer immobilization increased the number of aptamers on the electrode and detected HBsAg as low as 0.1 ng/mL on a linear regression coefficient curve within the range of 0.1 to 2 ng/mL. Furthermore, HBsAg-spiked serum increased the response of current without interference of proteins in the serum, indicating a specific detection of HBsAg. Control proteins HBeAg, HBeAb, HBsAb, HbcAb, albumin, and complementary aptamer did not change the current responses, confirming the specific detection of HBsAg by its aptamer. This HBsAg biosensor quantifies lower levels of HBsAg and helps to screen hepatitis B infection.
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Keywords
Aptamer,
Dielectric sensor,
Gold nanoparticles,
Hepatitis B,
Surface antigen